Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Vi vil især gerne takke medlemmerne af Gerber lab for tekniske instruktioner om deres eksperimentelle opsætning og kommentarer til manuskriptet. Vi takker også Lyubov Pankevych for flue pleje og vedligeholdelse af vildtype kantoner lager. Dette arbejde støttes af DFG tilskud TH1584/1-1, SNF tilskuddet 31003A_132812 / 1 og Zukunftskolleg fra University of Konstanz (alle til AST).

1. Forberedelse <ol><li> Drosophila vildtype-larver er rejst på 25 ° C og 60% -80% fugtighed i en 14/10 lys / mørkecyklus. Til styring af nøjagtige alder af larverne altid 20 hunner sættes med 10 hanner i et hætteglas (6 cm højde og 2,5 cm i diameter), der omfatter omkring 6 ml standard flue fødevarer. Fluer får lov til at lægge æg i 12 timer og overføres til et nyt hætteglas på den anden dag. 5-6 dage efter æglægning larver nå fodring 3. instar stadium hvis de tilvejebringes ved 2...

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	<li>Pawlow, I. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+Researches+on+Conditioned+Reflexes.">New Researches on Conditioned Reflexes.</a> Science. 58, 359-361 (1923).</li><li>Heisenberg, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mushroom+body+memoir:+from+maps+to+models.">Mushroom body memoir: from maps to models.</a> Nat. Rev. Neurosci. 4, 266-275 (2003).</li><li>Kandel, E. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+insights+into+behavior+and+learning.">Cellular insights into behavior and learning.</a> Harvey Lect. 73, 19-92 (1979).</li><li>Gerber, B., Tanimoto, H., Heisenberg, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+engram+found?+Evaluating+the+evidence+from+fruit+flies.">An engram found? Evaluating the evidence from fruit flies.</a> Curr. Opin. Neurobiol. 14, 737-744 (2004).</li><li>Milner, B., Squire, L. R., Kandel, E. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cognitive+neuroscience+and+the+study+of+memory.">Cognitive neuroscience and the study of memory.</a> Neuron. 20, 445-468 (1998).</li><li>Keene, A. C., Waddell, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drosophila+olfactory+memory:+single+genes+to+complex+neural+circuits.">Drosophila olfactory memory: single genes to complex neural circuits.</a> Nat. Rev. Neurosci. 8, (2007).</li><li>Duffy, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=GAL4+system+in+Drosophila:+a+fly+geneticist's+Swiss+army+knife.">GAL4 system in Drosophila: a fly geneticist's Swiss army knife.</a> Genesis. 34, 1-15 (2002).</li><li>Gerber, B., Stocker, R. F., Tanimura, T., Thum, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Smelling,+tasting,+learning:+Drosophila+as+a+study+case.">Smelling, tasting, learning: Drosophila as a study case.</a> Results Probl. Cell. Differ. 47, 139-185 (2009).</li><li>Gerber, B., Stocker, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+larva+as+a+model+for+studying+chemosensation+and+chemosensory+learning:+a+review.">The Drosophila larva as a model for studying chemosensation and chemosensory learning: a review.</a> Chem. Senses. 32, 65-89 (2007).</li><li>Venken, K. J., Simpson, J. H., Bellen, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+manipulation+of+genes+and+cells+in+the+nervous+system+of+the+fruit+fly.">Genetic manipulation of genes and cells in the nervous system of the fruit fly.</a> Neuron. 72, 202-230 (2011).</li><li>Gerber, B., Hendel, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Outcome+expectations+drive+learned+behaviour+in+larval+Drosophila.">Outcome expectations drive learned behaviour in larval Drosophila.</a> Proc. Biol. Sci. 273, 2965-2968 (2006).</li><li>Schleyer, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+behavior-based+circuit+model+of+how+outcome+expectations+organize+learned+behavior+in+larval+Drosophila.">A behavior-based circuit model of how outcome expectations organize learned behavior in larval Drosophila.</a> Learn Mem. 18, 639-653 (2011).</li><li>Pauls, D., Selcho, M., Gendre, N., Stocker, R. F., Thum, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drosophila+larvae+establish+appetitive+olfactory+memories+via+mushroom+body+neurons+of+embryonic+origin.">Drosophila larvae establish appetitive olfactory memories via mushroom body neurons of embryonic origin.</a> J. Neurosci. 30, 10655-10666 (2010).</li><li>Selcho, M., Pauls, D., Han, K. A., Stocker, R. F., Thum, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+dopamine+in+Drosophila+larval+classical+olfactory+conditioning.">The role of dopamine in Drosophila larval classical olfactory conditioning.</a> PLoS One. 4, e5897 (2009).</li><li>Neuser, K., Husse, J., Stock, P., Gerber, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Appetitive+olfactory+learning+in+Drosophila+larvae:effects+of+repetition,+reward+strength,+age,+gender,+assay+type+and+memory+span.">Appetitive olfactory learning in Drosophila larvae:effects of repetition, reward strength, age, gender, assay type and memory span.</a> Animal Behaviour. 69, 891-898 (2005).</li><li>Scherer, S., Stocker, R. F., Gerber, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Olfactory+learning+in+individually+assayed+Drosophila+larvae.">Olfactory learning in individually assayed Drosophila larvae.</a> Learn Mem. 10, 217-225 (2003).</li><li>Aceves-Pina, E. O., Quinn, W. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Learning+in+normal+and+mutant+Drosophila+larvae.">Learning in normal and mutant Drosophila larvae.</a> Science. 206, 93-96 (1979).</li><li>Heisenberg, M., Borst, A., Wagner, S., Byers, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drosophila+mushroom+body+mutants+are+deficient+in+olfactory+learning.">Drosophila mushroom body mutants are deficient in olfactory learning.</a> J. Neurogenet. 2, 1-30 (1985).</li><li>Khurana, S., Abu Baker, M. B., Siddiqi, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odour+avoidance+learning+in+the+larva+of+Drosophila+melanogaster.">Odour avoidance learning in the larva of Drosophila melanogaster.</a> J. Biosci. 34, 621-631 (2009).</li><li>Pauls, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electric+shock-induced+associative+olfactory+learning+in+Drosophila+larvae.">Electric shock-induced associative olfactory learning in Drosophila larvae.</a> Chem. Senses. 35, 335-346 (2010).</li><li>Eschbach, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Associative+learning+between+odorants+and+mechanosensory+punishment+in+larval+Drosophila.">Associative learning between odorants and mechanosensory punishment in larval Drosophila.</a> J. Exp. Biol. 214, 3897-3905 (2011).</li><li>von Essen, A. M., Pauls, D., Thum, A. S., Sprecher, S. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capacity+of+visual+classical+conditioning+in+Drosophila+larvae.">Capacity of visual classical conditioning in Drosophila larvae.</a> Behav. Neurosci. 125, 921-929 (2011).</li><li>Gerber, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visual+learning+in+individually+assayed+Drosophila+larvae.">Visual learning in individually assayed Drosophila larvae.</a> J. Exp. Biol. 207, 179-188 (2004).</li><li>Rohwedder, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nutritional+Value-Dependent+and+Nutritional+Value-Independent+Effects+on+Drosophila+melanogaster+Larval+Behavior.">Nutritional Value-Dependent and Nutritional Value-Independent Effects on Drosophila melanogaster Larval Behavior.</a> Chem. Senses. , (2012).</li><li>Lee, T., Lee, A., Luo, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+the+Drosophila+mushroom+bodies:+sequential+generation+of+three+distinct+types+of+neurons+from+a+neuroblast.">Development of the Drosophila mushroom bodies: sequential generation of three distinct types of neurons from a neuroblast.</a> Development. 126, 4065-4076 (1999).</li><li>Ito, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+organization+of+extrinsic+neurons+and+their+implications+in+the+functional+roles+of+the+mushroom+bodies+in+Drosophila+melanogaster+Meigen.">The organization of extrinsic neurons and their implications in the functional roles of the mushroom bodies in Drosophila melanogaster Meigen.</a> Learn Mem. 5, 52-77 (1998).</li><li>Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transmembrane/juxtamembrane+domain-dependent+Dscam+distribution+and+function+during+mushroom+body+neuronal+morphogenesis.">Transmembrane/juxtamembrane domain-dependent Dscam distribution and function during mushroom body neuronal morphogenesis.</a> Neuron. 43, 663-672 (2004).</li><li>Robertson, K., Mergliano, J., Minden, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissecting+Drosophila+embryonic+brain+development+using+photoactivated+gene+expression.">Dissecting Drosophila embryonic brain development using photoactivated gene expression.</a> Dev. Biol. 260, 124-137 (2003).</li><li>Zhou, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cooperative+functions+of+the+reaper+and+head+involution+defective+genes+in+the+programmed+cell+death+of+Drosophila+central+nervous+system+midline+cells.">Cooperative functions of the reaper and head involution defective genes in the programmed cell death of Drosophila central nervous system midline cells.</a> Proc. Natl. Acad. Sci. U.S.A. 94, 5131-5136 (1997).</li><li>Kitamoto, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conditional+modification+of+behavior+in+Drosophila+by+targeted+expression+of+a+temperature-sensitive+shibire+allele+in+defined+neurons.">Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons.</a> J. Neurobiol. 47, 81-92 (2001).</li><li>Schroll, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Light-induced+activation+of+distinct+modulatory+neurons+triggers+appetitive+or+aversive+learning+in+Drosophila+larvae.">Light-induced activation of distinct modulatory neurons triggers appetitive or aversive learning in Drosophila larvae.</a> Curr. Biol. 16, 1741-1747 (2006).</li><li>Rosenzweig, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+ortholog+of+vertebrate+TRPA1+regulates+thermotaxis.">The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis.</a> Genes Dev. 19, 419-424 (2005).</li><li>Baines, R. A., Uhler, J. P., Thompson, A., Sweeney, S. T., Bate, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Altered+electrical+properties+in+Drosophila+neurons+developing+without+synaptic+transmission.">Altered electrical properties in Drosophila neurons developing without synaptic transmission.</a> J. Neurosci. 21, 1523-1531 (2001).</li><li>Chen, Y. C., Mishra, D., Schmitt, L., Schmuker, M., Gerber, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+behavioral+odor+similarity+"space"+in+larval+Drosophila.">A behavioral odor similarity "space" in larval Drosophila.</a> Chem. Senses. 36, 237-249 (2011).</li><li>Saumweber, T., Husse, J., Gerber, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Innate+attractiveness+and+associative+learnability+of+odors+can+be+dissociated+in+larval+Drosophila.">Innate attractiveness and associative learnability of odors can be dissociated in larval Drosophila.</a> Chem. Senses. 36, 223-235 (2011).</li><li>von Essen, A. M., Pauls, D., Thum, A. S., Sprecher, S. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capacity+of+visual+classical+conditioning+in+Drosophila+larvae.">Capacity of visual classical conditioning in Drosophila larvae.</a> Behav. Neurosci. , (2011).</li><li>Honjo, K., Furukubo-Tokunaga, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+cAMP+response+element-binding+protein-dependent+medium-term+memory+by+appetitive+gustatory+reinforcement+in+Drosophila+larvae.">Induction of cAMP response element-binding protein-dependent medium-term memory by appetitive gustatory reinforcement in Drosophila larvae.</a> J. Neurosci. 25, 7905-7913 (2005).</li><li>Honjo, K., Furukubo-Tokunaga, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinctive+neuronal+networks+and+biochemical+pathways+for+appetitive+and+aversive+memory+in+Drosophila+larvae.">Distinctive neuronal networks and biochemical pathways for appetitive and aversive memory in Drosophila larvae.</a> J. Neurosci. 29, 852-862 (2009).</li><li>Khurana, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Olfactory+Conditioning+in+the+Third+Instar+Larvae+of+Drosophila+melanogaster+Using+Heat+Shock+Reinforcement.">Olfactory Conditioning in the Third Instar Larvae of Drosophila melanogaster Using Heat Shock Reinforcement.</a> Behav. Genet. 42, 151-161 (2012).</li><li>Tully, T., Cambiazo, V., Kruse, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Memory+through+metamorphosis+in+normal+and+mutant.">Memory through metamorphosis in normal and mutant.</a> 14, 68-74 (1994).</li><li>Michels, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+site+and+molecular+mode+of+synapsin+action+in+associative+learning.">Cellular site and molecular mode of synapsin action in associative learning.</a> Learn Mem. 18, 332-344 (2011).</li><li>Saumweber, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+and+synaptic+plasticity+are+impaired+upon+lack+of+the+synaptic+protein+SAP47.">Behavioral and synaptic plasticity are impaired upon lack of the synaptic protein SAP47.</a> J. Neurosci. 31, 3508-3518 (2011).</li><li>Pfeiffer, B. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Refinement+of+tools+for+targeted+gene+expression+in+Drosophila.">Refinement of tools for targeted gene expression in Drosophila.</a> Genetics. 186, 735-755 (2010).</li><li>Rosenzweig, M., Kang, K., Garrity, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinct+TRP+channels+are+required+for+warm+and+cool+avoidance+in+Drosophila+melanogaster.">Distinct TRP channels are required for warm and cool avoidance in Drosophila melanogaster.</a> Proc. Natl. Acad. Sci. U.S.A. 105, 14668-14673 (2008).</li></ol>

<html> Den beskrevne setup i Drosophila larver giver mulighed for undersøgelse af associative olfaktoriske læring inden et sammenligneligt elementær hjerne. Fremgangsmåden er simpel, billig, let at etablere sig i et laboratorium og ikke kræver højteknologisk udstyr 9. Vi præsenterer en version af eksperimentet for at undersøge appetitive associativ indlæring forstærket af fructose belønning 11. Den beskrevne setup er baseret på en række parametriske undersøgelser, omfattende u...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalognummer </td><td> CAS-nummer </td></tr><tr><td> Fruktose </td><td> Sigma </td><td> 47.740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71.350 </td><td> 7647-14-5 </td></tr><tr><td> Agarose </td><td> Sigma </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1-octanol </td><td> Sigma </td><td> 12.012 </td><td> 111-87-5 </td></tr><tr><td> Amylacetate </td><td> Sigma </td><td> 46.022 </td><td> 628-63-7 </td></tr><tr><td> Paraffinolie </td><td> Sigma </td><td> 18.512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

I det følgende beskriver vi de metodemæssige enkeltheder i appetitive associative olfaktoriske læring i Drosophila larver. Opsætningen, i kombination med genetiske forstyrrelser, giver et håndtag til at analysere de neuronale og molekylær grundlæggende elementer specifikt associative læring i en simpel larve hjerne. Organismer kan bruge tidligere erfaringer til at justere nuværende adfærd. En sådan erhvervelse af adfærdsmæssige potentiale kan defineres som læring, og de ​​fysiske baser af disse potentialer som hukommelses spor 1-4. Neuroforskere forsøger at forstå, hvordan disse processer er organiseret i form af molekylære og neuronale ændringer i hjernen ved hjælp af en række fremgangsmåder i modelorganismer lige fra insekter til vertebrater 5,6. For sådanne bestræbelser er det nyttigt at anvende modelsystemer, der er enkle og eksperimentelt tilgængelige. Drosophila Larven har vist sig at opfylde disse krav er baseret påtilgængeligheden af robuste adfærdsmæssige assays, eksistensen af en bred vifte af transgene teknikker og den elementære organisation af nervesystemet, der kun omfatter omkring 10.000 neuroner (om end med nogle indrømmelser: kognitive begrænsninger, få adfærdsmæssige muligheder, og rigdom af erfaringer tvivlsom) 7-10 . Drosophila larver kan danne associationer mellem lugte og appetitive gustatoriske forstærkning ligesom sukker 11-14. I et standardassay, der er etableret i laboratoriet af B. Gerber dyrene får en to-lugt gensidig træning: En første gruppe af larver udsættes for en lugt A sammen med en gustatoriske forstærker (sukker belønning) og efterfølgende udsat for en lugt B uden armering 9. Imens en anden gruppe af larver modtager gensidig træning mens du oplever lugt A uden forstærkning og efterfølgende blive udsat for lugt B med forstærkning (sukker belønning). I det følgende begge grupper er tesTed for deres præference mellem de to lugte. Relativt højere præferencer for det belønnet lugt afspejler associative læring - præsenteret som en performance index (PI). Konklusionen vedrørende associative karakter af ydeevne indeks er overbevisende, fordi bortset fra til uforudsete mellem lugte og tastants, andre parametre, såsom lugt og belønning eksponering, passage af tid og håndtering ikke adskiller mellem de to grupper 9.

Figur 1A viser en oversigt over de eksperimentelle procedurer for larvestadiet lugtesansen associativ læring. Ved at parre en af ​​de to præsenterede lugte med en sukker belønning larver erhverve adfærd potentialet til at udtrykke et attraktivt respons mod belønnet lugt i forhold til den ubelønnet lugt. To grupper af larver er altid trænet af enten parre forstærker med lugt OLT eller AM. Udførelsen (PI) måler associative funktion som forskellen frem mellem de gensidigt uddannede grupper. <...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila larver er i stand til at knytte lugt stimuli med gustatoriske belønning. Her beskriver vi en simpel adfærdsmæssige paradigme, der tillader analyse af appetitive associativ olfaktoriske læring.

Appetitive Associativ olfaktorisk læring i<em&gt; Drosophila</em&gt; Larver

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

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Appetitive Associative Olfactory Learning in Drosophila Larvae

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In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualisering af proprioceptorer i<em&gt; Drosophila</em&gt; Larver og pupper

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

Drosophila Voksen Olfactory Shock læring

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay til at studere Medfødte og Circadian Reguleret Photobehavior i<em&gt; Drosophila</em&gt; Larver

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Hele Mount immunolabeling af olfaktoriske receptor neuroner i<em&gt; Drosophila</em&gt; Antenne

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Elektrofysiologiske Metode til Recording Intracellulær Voltage Responses af<em&gt; Drosophila</em&gt; Fotoreceptorer og interneuroner til Light Stimuli<em&gt; In vivo</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

Novel analyse for Cold Nociception i<em&gt; Drosophila</em&gt; Larver

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

En objektiv og reproducerbare Test af olfaktoriske læring og diskrimination i mus

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...