Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Nous tenons particulièrement à remercier les membres du laboratoire Gerber pour obtenir des instructions techniques sur leur dispositif expérimental et commentaires sur le manuscrit. Nous remercions également Lyubov Pankevych pour les soins et l&#39;entretien de la mouche du type cantons stock sauvage. Ce travail est soutenu par la DFG TH1584/1-1 subvention, la subvention SNF 31003A_132812 / 1 et le Zukunftskolleg de l&#39;Université de Konstanz (tout à AST).

1. Préparation <ol><li> Drosophile de type sauvage larves sont élevées à 25 ° C et 60% -80% d&#39;humidité dans un cycle de 14/10 lumière / obscurité. Pour le contrôle de l&#39;âge exact des larves toujours 20 femelles sont mises avec 10 hommes dans un flacon (6 cm de hauteur et 2,5 cm de diamètre) qui comprend environ 6 ml de nourriture volée standard. Les mouches sont autorisés à pondre des œufs pendant 12 heures et sont transférés dans un nouveau flacon, le deuxième jour. 5-6 jours après...

<ol>
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<html> La configuration décrite dans les larves de drosophile permet d&#39;enquêter sur l&#39;apprentissage olfactif associatif au sein d&#39;un cerveau comparable élémentaire. L&#39;approche est simple, pas cher, facile à mettre en place dans un laboratoire et ne nécessite pas de matériel de haute technologie 9. Nous présentons une version de l&#39;expérience, d&#39;étudier appétitive apprentissage associatif renforcé par la récompense fructose 11. La configuration décrite es...

<table border="1"><tbody><tr><td> Nom du réactif </td><td> Entreprise </td><td> Numéro de catalogue </td><td> Numéro CAS </td></tr><tr><td> Fructose </td><td> Sigma </td><td> 47740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71350 </td><td> 7647-14-5 </td></tr><tr><td> Agarose </td><td> Sigma </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1-octanol </td><td> Sigma </td><td> 12012 </td><td> 111-87-5 </td></tr><tr><td> Amyle </td><td> Sigma </td><td> 46022 </td><td> 628-63-7 </td></tr><tr><td> Pétrole </td><td> Sigma </td><td> 18512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

Dans ce qui suit, nous décrivons les détails méthodologiques de l&#39;apprentissage olfactif associatif appétit chez les larves de drosophile. L&#39;installation, en association avec d&#39;interférence génétique, fournit une poignée d&#39;analyser les bases neuronales et moléculaire de l&#39;apprentissage associatif spécifiquement à une réflexion simple larvaire. Les organismes peuvent utiliser l&#39;expérience passée pour ajuster le comportement présent. Une telle acquisition du potentiel de comportement peut être définie comme l&#39;apprentissage et les bases physiques de ces potentiels que les traces mnésiques 1-4. Les neuroscientifiques essayer de comprendre comment ces processus sont organisés en fonction des changements moléculaires et neuronale dans le cerveau en utilisant une variété de méthodes dans des organismes modèles, allant des insectes aux vertébrés 5,6. Pour ces efforts, il est utile d&#39;utiliser des systèmes modèles simples et accessibles expérimentalement. La larve Drosophila s&#39;est avéré répondre à ces exigences fondées surla disponibilité de solides analyses comportementales, l&#39;existence d&#39;une variété de techniques transgéniques et l&#39;organisation élémentaire du système nerveux comprenant seulement environ 10.000 neurones (quoique avec quelques concessions: limitations cognitives, comportementales, peu d&#39;options et de la richesse de l&#39;expérience douteuse) 7-10 . Larves de drosophile peuvent créer des associations entre les odeurs et appétitive renforcement gustative comme le sucre 11-14. Dans un essai standard, mis en place dans le laboratoire de B. Gerber, les animaux reçoivent une formation de deux odeurs réciproque: Un premier groupe de larves est exposé à une odeur A avec un renforçateur gustative (récompense sucre) et est ensuite exposé à une odeur B sans armature 9. Pendant ce temps un deuxième groupe de larves reçoit une formation réciproque tout en éprouvant une odeur sans armature et étant ensuite exposée à odeurs B avec renforcement (récompense sucre). Dans ce qui suit les deux groupes sont tested pour leur préférence entre les deux odeurs. Préférences relativement plus élevés pour l&#39;odeur récompensés reflètent l&#39;apprentissage associatif - présenté comme un indice de performance (PI). La conclusion quant à la nature associative de l&#39;indice de performance est convaincante, car en dehors de la contingence entre les odeurs et sapides, d&#39;autres paramètres, tels que l&#39;odeur et l&#39;exposition de récompense, le passage du temps et de manipulation ne diffèrent pas entre les deux groupes 9.

La figure 1A montre une vue d&#39;ensemble des procédures expérimentales pour des larves apprentissage associatif olfactif. En couplant une des deux odeurs présentées avec une récompense larves sucre acquérir le comportement potentiel d&#39;exprimer une réponse intéressante à l&#39;odeur récompensés par rapport à l&#39;odeur récompense. Deux groupes de larves sont toujours formés soit par l&#39;appariement du renfort de l&#39;odeur ou du PTOM AM. L&#39;indice de performance (PI) mesure la ...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Larves de drosophile sont capables de s&#39;associer avec des stimuli odeur récompense gustative. Nous décrivons ici un paradigme simple de comportement qui permet l&#39;analyse de l&#39;apprentissage olfactif associatif appétitive.

Appétitive apprentissage olfactif associatif en<em&gt; Drosophila</em&gt; Les larves

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

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Appetitive Associative Olfactory Learning in Drosophila Larvae

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Méthodes locales et globales de l&#39;évaluation de nociception thermique dans<em&gt; Drosophila</em&gt; Les larves

In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualisation des propriocepteurs dans<em&gt; Drosophila</emLes larves&gt; et pupes

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

Drosophile adulte olfactive apprentissage choc

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Essai de préférence la lumière pour étudier innée et circadien Photobehavior Réglementé<em&gt; Drosophila</em&gt; Les larves

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Tout le mont immunomarquage des récepteurs olfactifs neurones dans le<em&gt; Drosophila</em&gt; Antenne

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Méthode électrophysiologique de réponses d&#39;enregistrement intracellulaire tension de<em&gt; Drosophila</em&gt; Photorécepteurs et Interneurones à Light Stimuli<em&gt; In Vivo</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

Nouveau test pour froid dans nociception<em&gt; drosophile</em&gt; larves

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

Un Test objectif et reproductible d’apprentissage olfactif et de la Discrimination chez les souris

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...