Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

我々は、特に彼らの実験設定と原稿のコメントに関する技術的な手順については、ガーバー·ラボのメンバーに感謝したいと思います。我々はまた、野生型カントン株式のフライケアとメンテナンスのためリュボフPankevychに感謝します。この作品は、DFG助成TH1584/1-1、SNFの助成31003A_132812 / 1およびコンスタンツ大学のZukunftskolleg（全ASTに）によってサポートされています。

1。準備<ol><li> ショウジョウバエの野生型幼虫は10分の14明/暗サイクルにおける℃、60％〜80％相対湿度は25で提起されています。幼虫の正確な年齢を制御するために、常に20人の女性は、標準的なフライ食品の約6mlを含む1バイアル（6cmの高さと直径2.5cm）に10人の男性に置かれます。ハエは12時間の卵を産むために許容されていて、二日目には新しいバイアルに移しています。 25℃で飼?...

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<html> ショウジョウバエの幼虫の記載されているセットアップは比較的初歩脳内で連想嗅覚学習の調査を行うことが可能。アプローチでは、実験室で確立するために、シンプルな安い、簡単ですし、ハイテク機器9を必要としません。我々は、果糖の報酬11によって補強食欲連想学習を研究するために、実験のバージョンを提示します。記載されているセットアッ...

<table border="1"><tbody><tr><td> 試薬の名称 </td><td> 会社 </td><td> カタログ番号 </td><td> CAS番号 </td></tr><tr><td>果糖</td><td>シグマ</td><td> 47740 </td><td> 57-48-7 </td></tr><tr><td> NaClを</td><td>フルカ</td><td> 71350 </td><td> 7647-14-5 </td></tr><tr><td>アガロース</td><td>シグマ</td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1 - オクタノール</td><td>シグマ</td><td> 12012 </td><td> 111-87-5 </td></tr><tr><td>アミル</td><td>シグマ</td><td> 46022 </td><td> 628-63-7 </td></tr><tr><td>灯油</td><td>シグマ</td><td> 18512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

以下では、 ショウジョウバエの幼虫の食欲連想嗅覚学習の方法論の詳細について説明します。セットアップでは、遺伝的干渉との組み合わせで、シンプルな幼虫の脳に特異的に連想学習の神経細胞と分子のファンダメンタルズを分析するためのハンドルを提供します。 生物が存在する動作を調整するために過去の経験を使用することができます。潜在的な行動のような買収は、メモリ·トレース1〜4として学習し、これらのポテンシャルの物理的な拠点として定義することができます。神経科学者は、これらのプロセスは、昆虫から脊椎動物5,6に至るまでモデル生物での様々な方法を用いて脳内の分子やニューロンの変化の観点でどのように構成されるかを理解しよう。そのような努力のためにそれは簡単で、実験的にアクセス可能なモデルシステムを使用すると便利です。 ショウジョウバエの幼虫はに基づいて、これらの要求を満たすことが判明しました7-10：堅牢な行動アッセイ、トランスジェニック技術の多様性と唯一の約10,000ニューロン（認知限界、いくつかの行動オプション、および疑わしい経験の豊かある程度の譲歩とはいえ）を含む神経系の基本組織の存在の可用性。 ショウジョウバエの幼虫は臭いと砂糖11月14日のような食欲の味覚補強の間のアソシエーションを形成することができる。 B.ガーバーの研究室で確立された標準的アッセイでは、動物は、二臭逆数訓練を受ける：幼虫の最初のグループは、味覚の補強材（砂糖の報酬）と一緒に臭気にさらされ、その後臭気にさらされている補強なしの9のB。補強なし臭気が発生し、その後、（砂糖報酬）補強臭気Bにさらされながら、一方で幼虫の2番目のグループは、相互の研修を受ける。以下では、両グループはTESアール2悪臭の間に自分の好みのためのテッド。パフォーマンス指標（PI）として提示 - 報わ臭気のために比較的高い嗜好は連想的学習を反映しています。パフォーマンス·インデックスの連想性質に関する結論がバラバラに臭いや味物質、そのような臭気と報酬の露出、時間と処理の経過2グループ9の間に違いはありませんなどの他のパラメータの間に不測の事態からあるので、説得力がある。

図1Aは、幼虫の嗅覚連合学習の実験手順の概要を示しています。砂糖報酬幼虫を2提示臭いのいずれかを組み合わせることで報われない匂いに比べて報わ匂いに向かって魅力的な応答を表現する潜在的な動作を取得します。幼虫の2つのグループが常にどちら臭気10月またはAMと強化子を組み合わせることで訓練されています。性能指標（PI）は相互に訓練されたグループ間の嗜好の...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

ショウジョウバエの幼虫は味覚報酬で匂い刺激を関連付けることができます。ここでは、食欲の連想嗅覚学習の分析を可能にする単純な行動パラダイムを記述します。

で食欲連想嗅覚学習<em&gt;ショウジョウバエ</em&gt;幼虫

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

Research

JoVE Journal

Neuroscience

Appetitive Associative Olfactory Learning in Drosophila Larvae

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegansの正ブタノンの学習、短期、および長期連想記憶アッセイ

The memory of experiences and learned information is critical for organisms to make choices that aid their survival. C. elegans navigates its environment ...

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

でアッセイする運動、学習、記憶障害ショウジョウバエ</em神経変性の>モデル

Advances in genetic methods have enabled the study of genes involved in human neurodegenerative diseases using Drosophila as a model system1. Most of ...

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

に温痛覚を評価するためのローカルおよびグローバルメソッド<em&gt;ショウジョウバエ</em&gt;幼虫

In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

でProprioceptorsの可視化<em&gt;ショウジョウバエ</em&gt;幼虫および蛹

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

ショウジョウバエアダルト嗅覚ショックラーニング

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

に自然免疫とサーカディアン規制Photobehaviorを勉強する光プアッセイ<em&gt;ショウジョウバエ</em&gt;幼虫

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

における嗅覚受容ニューロンのホールマウント免疫標識<em&gt;ショウジョウバエ</em&gt;アンテナ

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

の記録細胞内の電圧応答のための電気生理学的手法<em&gt;ショウジョウバエ</em&gt;光刺激に感光体と介在ニューロン<em&gt;インビボ</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

冷痛覚のための新規アッセイ<em&gt;ショウジョウバエ</em&gt;幼虫

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

嗅覚学習とマウスにおける差別の客観的かつ再現性のあるテスト

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...