Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

우리는 특히 실험 설정 및 원고에 대한 의견에 대한 기술 지침에 대한 거버 연구소의 구성원을 감사드립니다. 우리는 또한 야생 형 CantonS 주식의 플라이 관심과 유지 보수를 위해 류 보프 Pankevych 감사드립니다. 이 작품은 DFG 부여 TH1584/1-1, SNF 부여 31003A_132812 / 1 콘 스탄 츠 (Konstanz) 대학의 Zukunftskolleg (모든 대서양 표준시로)에 의해 지원됩니다.

1. 준비하기 <ol><li> Drosophila 야생 형 유충은 25 제기 ° C와 10분의 14 라이트 / 어두운주기에 60% -80 %의 습도. 항상 20 여자는 표준 플라이 식품의 약 6 ML을 포함 한 병 (6cm의 높이와 2.5 cm 직경)에 10 남성시킨 애벌레의 정확한 연령을 제어하십시오. 파리는 12 시간에 알을 낳기 위해 허용하고 두 번째 날에 새로운 유리 병으로 전송됩니다. 25 ° C에서 제기되어, 지금은 행동 실험을 위해 사용할 수?...

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<html> Drosophila의 애벌레의 설명 설정은 comparably 초등학교 두뇌 속에 연관 후각 학습의 조사 할 수 있습니다. 방법은 실험실에서 확립 할, 간단한 저렴 용이하고 첨단 장비를 구가 필요하지 않습니다. 우리는 과당 보상 11에 의해 강화 appetitive 연관 학습을 연구하는 실험 버전을 제시한다. 설명 설정이 종합적으로 교육 시험의 수가 변화, 단일 검정 대 질량 분석, 보존 기간, 사?...

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td><td> CAS 번호 </td></tr><tr><td> 프 럭토 오스 </td><td> 시그마 </td><td> 47,740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71,350 </td><td> 7647-14-5 </td></tr><tr><td> 아가로 오스 </td><td> 시그마 </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1 octanol </td><td> 시그마 </td><td> 12,012 </td><td> 111-87-5 </td></tr><tr><td> Amylacetate </td><td> 시그마 </td><td> 46,022 </td><td> 628-63-7 </td></tr><tr><td> 파라핀 유 </td><td> 시그마 </td><td> 18,512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

다음에 우리는 Drosophila의 애벌레에 appetitive 연관 후각 학습의 방법론 자세한 내용을 설명합니다. 설정은, 유전자 간섭와 함께, 간단한 애벌레의 뇌에 구체적으로 연관 학습의 neuronal와 분자 기초를 분석 할 수있는 핸들을 제공합니다. 생물은 현재 동작을 조정하는 과거의 경험을 사용할 수 있습니다. 행동 잠재력 이러한 인수는 학습으로 정의하고, 메모리 트레이스 1-4 이러한 가능성의 물리적 기반. 할 수 있습니다 Neuroscientists이 프로세스가 곤충에서 vertebrates 5,6에 이르기까지 다양한 모델 생물의 다양한 방법을 사용하여 뇌의 분자와 neuronal 변화의 관점에서 구성하는 방법을 이해하려고합니다. 이러한 노력에 대한 간단하고 실험적으로 액세스 할 수 있습니다 모델 시스템을 사용하는 도움이됩니다. Drosophila의 유충이에 따라 이러한 요구를 충족하기 위해가되었습니다7-10 강력한 행동 assays, 유전자 변형 기술의 다양성과 약 10,000 뉴런을 (인지 제한, 몇 가지 행동 옵션 및 의심 경험 풍부한 일부 할인 혜택이있는에도 불구하고)를 포함하는 신경계의 초등학교 조직의 존재의 가용성 . Drosophila의 애벌레는 악취, 설탕 11-14 같은 appetitive gustatory 강화 사이의 연관 관계를 형성 할 수있다. 표준 검정에서 B. 거버의 연구실에 설립, 동물은 두 냄새 상호 교육을받을 : 애벌레의 첫 번째 그룹은 gustatory reinforcer (설탕 보상)와 함께 냄새에 노출되어 이후 냄새에 노출되어 강화 아홉없이 B. 보강하지 않고 냄새가 발생하고 이후 강화 (설탕 보상)와 악취 B에 노출되는 동안 한편 애벌레의 두 번째 그룹은 상호 교육을받습니다. 다음의 두 그룹 tes 아르두 냄새 사이의 환경 설정에 대한 테드. 보상 냄새에 대한 상대적으로 높은 환경 설정 연관 학습을 반영 - 성능 지수 (PI)로 제시했다. 성능 색인의 결합 특성에 대한 결론은 떨어져 냄새와 tastants 등 냄새 및 보상에 노출, 시간 및 취급의 통로 두 그룹 9 다를하지 않는 등 다른 매개 변수 사이의 우발적의 때문에 눈길을 끄는 것입니다.

그림 1A는 애벌레의 후각 연관 학습을위한 실험 절차의 개요를 보여줍니다. 설탕 보상 애벌레와 두 제시 냄새 중 하나를 페어링하면 보수없는 냄새에 비해 보상 냄새에 대한 매력적인 응답을 표현할 수있는 잠재적 인 행동을 습득. 애벌레의 두 그룹은 항상 두 냄새 10월 또는 AM으로 reinforcer을 페어링하여 훈련하고 있습니다. 성능 지수 (PI)는 서로 훈련 집단 간의 선호의 차이로 연관 기...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila의 애벌레는 gustatory 보상과 냄새 자극을 연결 할 수 있습니다. 여기 appetitive 연관 후각 학습의 분석을 할 수있는 간단한 행동 패러다임을 설명합니다.

에 Appetitive 연관 강한 학습<em&gt; Drosophila</em&gt; 애벌레

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

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Neuroscience

Appetitive Associative Olfactory Learning in Drosophila Larvae

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