Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Vi ønsker spesielt å takke medlemmene av Gerber lab for tekniske instruksjoner om sin eksperimentelle oppsett og kommentarer til manuskriptet. Vi vil også takke Lyubov Pankevych for fly omsorg og vedlikehold av villtype kantoner lager. Dette arbeidet støttes av DFG tilskuddet TH1584/1-1, SNF stipend 31003A_132812 / 1 og Zukunftskolleg ved University of Konstanz (alle til AST).

1. Forberedelse <ol><li> Drosophila villtype larver er hevet ved 25 ° C og 60% -80% fuktighet i en 14/10 lys / mørke syklus. For kontroll den eksakte alderen larvene alltid 20 hunner settes med 10 hanner inn en ampulle (6 cm høyde og 2,5 cm diameter) som omfatter ca 6 ml standard flue mat. Fluer er lov til å legge egg i 12 timer og overføres til et nytt hetteglass på den andre dagen. 5-6 dager etter egglegging larver nå fôring 3. instar stadium dersom hevet ved 25 ° C og kan nå brukes for...

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<html> Den beskrevne oppsettet i Drosophila larver tillater undersøkelse av assosiative olfactory læring innenfor et relativt elementær hjerne. Tilnærmingen er enkel, billig, lett å etablere i et laboratorium og krever ikke high-tech utstyr 9. Vi presenterer en versjon av forsøket, for å studere appetitive assosiativ læring forsterket av fruktose belønning 11. Den beskrevne oppsettet er basert på en serie av parametrical studier som omfattende undersøkt variasjoner i antall treni...

<table border="1"><tbody><tr><td> Navnet på reagensen </td><td> Selskapet </td><td> Katalognummer </td><td> CAS nummer </td></tr><tr><td> Fruktose </td><td> Sigma </td><td> 47740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71350 </td><td> 7647-14-5 </td></tr><tr><td> Agarose </td><td> Sigma </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1-oktanol </td><td> Sigma </td><td> 12012 </td><td> 111-87-5 </td></tr><tr><td> Amylacetate </td><td> Sigma </td><td> 46022 </td><td> 628-63-7 </td></tr><tr><td> Parafinolje </td><td> Sigma </td><td> 18512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

I det følgende beskriver vi de metodiske detaljer om appetitive assosiativ olfactory læring i Drosophila larver. Oppsettet, i kombinasjon med genetisk forstyrrelse, gir et håndtak å analysere neuronal og molekylære grunnleggende av spesifikt assosiativ læring i en enkel larve hjerne. Organismer kan bruke tidligere erfaringer til å justere dagens atferd. Slikt erverv av atferdsmessige potensial kan defineres som læring, og de ​​fysiske grunnlaget for disse potensialene som minne spor 1-4. Nevrologer prøve å forstå hvordan disse prosessene er organisert i form av molekylære og neuronal endringer i hjernen ved hjelp av en rekke metoder i modellorganismer som spenner fra insekter til virveldyr 5,6. For slike bestrebelser er det nyttig å bruke modellen systemer som er enkle og eksperimentelt tilgjengelig. Drosophila larve har vist seg å tilfredsstille disse kravene basert påtilgjengeligheten av robuste atferdsmessige analyser, eksistensen av en rekke transgene teknikker og elementær organisering av nervesystemet som består av bare ca 10000 nevroner (riktignok med noen innrømmelser: kognitive begrensninger, få atferdsmessige alternativer og rikdom av erfaring tvilsom) 7-10 . Drosophila larver kan danne assosiasjoner mellom lukt og appetitive gustatory forsterkning som sukker 11-14. I et standard assay, etablert i laboratoriet av B. Gerber, dyr får en to-lukt gjensidig opplæring: en første gruppe av larver er eksponert til en lukt En sammen med en gustatory reinforcer (sukker belønning) og er deretter utsatt for en lukt B uten forsterkning 9. Samtidig vil en annen gruppe av larver mottar resiprok trening mens opplever lukt A uten armering og deretter blir utsatt for lukt B med armering (sukker belønning). I det følgende begge gruppene er tested for deres preferanse mellom de to lukt. Relativt høyere innstillinger for belønnet lukt gjenspeiler assosiativ læring - presentert som en forestilling indeks (PI). Konklusjonen om assosiative natur ytelsen indeksen er overbevisende, fordi bortsett fra beredskap mellom lukt og tastants, andre parametere, slik som lukt og belønning eksponering, passering av tid og håndtering skiller seg ikke mellom de to gruppene 9.

Figur 1a viser en oversikt over de eksperimentelle prosedyrer for larve olfactory assosiativ læring. Ved sammenkobling av en av to presentert lukt med en sukker belønning larver erverve atferden potensial å uttrykke en attraktiv respons mot belønnet lukt i forhold til unrewarded lukt. To grupper av larver er alltid trent av enten sammenkobling forsterkeren med lukt oktober eller AM. Ytelsen indeks (PI) måler assosiative funksjonen som forskjellen i preferanse mellom gjensidig trente gruppene. <...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila larver er i stand til å knytte lukt stimuli med gustatory belønning. Her beskriver vi en enkel atferdsmessige paradigme som tillater analyse av appetitive assosiativ olfactory læring.

Appetitive Assosiative Olfactory læring i<em&gt; Drosophila</em&gt; Larver

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

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Appetitive Associative Olfactory Learning in Drosophila Larvae

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Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Lett preferanse analysen å studere medfødte og Circadian Regulert Photobehavior i<em&gt; Drosophila</em&gt; Larver

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Hele Mount immunolabeling av olfactory Receptor nevroner i<em&gt; Drosophila</em&gt; Antenne

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Elektro Metode for opptak Intracellulær spenningsreaksjoner av<em&gt; Drosophila</em&gt; Fotoreseptorer og interneurons til lys stimuli<em&gt; I Vivo</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

Novel analyse for Cold nociception i<em&gt; Drosophila</em&gt; Larver

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

En objektiv og reproduserbar Test av Olfactory læring og diskriminering i mus

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...