Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

En especial queremos agradecer a los miembros del laboratorio Gerber para obtener instrucciones técnicas para su montaje experimental y los comentarios sobre el manuscrito. También agradecemos a Lyubov Pankevych para el cuidado de la mosca y el mantenimiento del tipo salvaje acciones cantones. Este trabajo es apoyado por la DFG TH1584/1-1 subvención, la subvención SNF 31003A_132812 / 1 y el Zukunftskolleg de la Universidad de Konstanz (todos AST).

1. Preparación <ol><li> Drosophila salvaje larvas se crían a 25 ° C y 60% -80% de humedad en un ciclo de 14/10 luz / oscuridad. Para el control de la edad exacta de las larvas siempre se ponen 20 hembras y 10 machos en un vial (6 cm de altura y 2,5 cm de diámetro) que incluye aproximadamente 6 ml de alimento mosca estándar. Las moscas se les permite poner huevos durante 12 horas y se transfieren a un nuevo vial en el segundo día. 5-6 días después de la puesta de huevos larvas de llegar a la alimentaci...

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<html> La configuración descrita en larvas de Drosophila permite la investigación de aprendizaje olfativo asociativo dentro de un cerebro comparativamente elemental. El enfoque es simple, barato, fácil de establecer en un laboratorio y no requiere de equipos de alta tecnología 9. Se presenta una versión del experimento para estudiar el aprendizaje apetitivo asociativo reforzada por recompensa fructosa 11. La configuración descrita se basa en una serie de estudios paramétricos que exh...

<table border="1"><tbody><tr><td> Nombre del reactivo </td><td> Empresa </td><td> Número de catálogo </td><td> Número del CAS </td></tr><tr><td> Fructosa </td><td> Sigma </td><td> 47740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71350 </td><td> 7647-14-5 </td></tr><tr><td> Agarosa </td><td> Sigma </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1-octanol </td><td> Sigma </td><td> 12012 </td><td> 111-87-5 </td></tr><tr><td> Amilacetato </td><td> Sigma </td><td> 46022 </td><td> 628-63-7 </td></tr><tr><td> Queroseno </td><td> Sigma </td><td> 18512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

A continuación se describen los detalles metodológicos de aprendizaje asociativo apetitiva olfativa de larvas de Drosophila. La configuración, en combinación con la interferencia genética, proporciona un identificador para analizar los fundamentos neuronales y moleculares de aprendizaje asociativo específicamente en un cerebro de larvas simple. Los organismos pueden utilizar la experiencia pasada para ajustar el comportamiento presente. Tal adquisición de potencial de comportamiento puede ser definido como el aprendizaje, y las bases físicas de estos potenciales como trazas de memoria 1-4. Los neurocientíficos tratar de entender cómo estos procesos están organizados en función de los cambios moleculares y neuronales en el cerebro mediante el uso de una variedad de métodos en organismos modelos que van desde los insectos a vertebrados 5,6. Para tales esfuerzos, es útil utilizar modelos de sistemas que son simples y accesibles experimentalmente. La larva de Drosophila se ha convertido en satisfacer estas demandas sobre la base dela disponibilidad de potentes ensayos de comportamiento, la existencia de una variedad de técnicas transgénicas y la organización básica del sistema nervioso que comprende sólo unas 10.000 neuronas (aunque con algunas concesiones: las limitaciones cognitivas, pocas opciones de comportamiento, y la riqueza de la experiencia cuestionable) 7-10 . Larvas de Drosophila pueden formar asociaciones entre los olores y el refuerzo del apetito gustativo como el azúcar 11-14. En un ensayo estándar, establecido en el laboratorio de B. Gerber, los animales reciben una de dos olor formación recíproca: Un primer grupo de larvas está expuesto a un olor A, junto con un reforzador gustativa (recompensa azúcar) y es posteriormente expuesto a un olor B sin refuerzo 9. Mientras tanto, un segundo grupo de larvas recibe formación recíproca, mientras que experimentan olor A sin refuerzo y posteriormente expuestos a B con olor refuerzo (recompensa azúcar). En la siguiente ambos grupos son TESdo por su preferencia entre los dos olores. Preferencias relativamente altos para el olor recompensado reflejar el aprendizaje asociativo - presentado como un índice de rendimiento (PI). La conclusión sobre la naturaleza asociativa del índice de rendimiento es convincente, ya que además de la contingencia entre los olores y los estimulantes del gusto, otros parámetros, como el olor y la exposición recompensa, el paso del tiempo y la manipulación no difieren entre los dos grupos de 9.

La figura 1A muestra una visión general de los procedimientos experimentales para las larvas aprendizaje asociativo olfativo. Por el emparejamiento de una de las dos olores presentados con una recompensa larvas azúcar adquirir el comportamiento potencial de expresar una respuesta atractiva hacia el olor recompensado en comparación con el olor sin recompensa. Dos grupos de larvas están siempre formados por cualquiera de emparejamiento el reforzador con el olor octubre o AM. El índice de rendimiento ...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Larvas de Drosophila son capaces de asociarse con estímulos de olor recompensa gustativa. Aquí se describe un sencillo paradigma de comportamiento que permite el análisis de apetito aprendizaje olfativo asociativo.

Apetitivo aprendizaje asociativo olfativa en<em&gt; Drosophila</em&gt; Las larvas

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

Research

JoVE Journal

Neuroscience

Appetitive Associative Olfactory Learning in Drosophila Larvae

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegans de aprendizaje positivo butanona, a corto plazo y largo plazo Ensayos de la memoria asociativa

The memory of experiences and learned information is critical for organisms to make choices that aid their survival. C. elegans navigates its environment ...

Investigación

Neurociencias

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

Locomotor ensayo, aprendizaje, memoria y déficit en Drosophila Los modelos de neurodegeneración

Advances in genetic methods have enabled the study of genes involved in human neurodegenerative diseases using Drosophila as a model system1. Most of ...

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Métodos locales y globales de evaluación de nocicepción térmica en<em&gt; Drosophila</em&gt; Las larvas

In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualización de los propioceptores<em&gt; Drosophila</em&gt; Las larvas y pupas

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

Drosophila Adulto olfativa Choque Aprendizaje

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Ensayo Preferencia luz para estudiar innata y circadiano Photobehavior Regulado de<em&gt; Drosophila</em&gt; Las larvas

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Todo el montaje de Immunolabeling olfativa del receptor de las neuronas en el<em&gt; Drosophila</em&gt; Antena

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Método electrofisiológico para registrar las respuestas de voltaje intracelular<em&gt; Drosophila</em&gt; Los fotorreceptores y interneuronas a la luz Estímulos<em&gt; En Vivo</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

Ensayo novedoso para la nocicepción en frío<em&gt; Drosophila</em&gt; Las larvas

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

Una prueba objetiva y Reproducible de aprendizaje olfativo y discriminación en ratones

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...