Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Vi vill särskilt tacka medlemmarna i Gerber labb för tekniska instruktioner på deras experimentuppställning och kommentarer om manuskriptet. Vi tackar också Lyubov Pankevych för flyga skötsel och underhåll av vildtyp kantoner lager. Detta arbete stöds av DFG bidraget TH1584/1-1, SNF bidraget 31003A_132812 / 1 och Zukunftskolleg vid universitetet i Konstanz (alla till AST).

1. Förberedelser <ol><li> Drosophila vildtyp larver höjs vid 25 ° C och 60% -80% fuktighet i en 14/10 ljus / mörker cykel. För styrning av exakta ålder larverna alltid 20 honor placeras med 10 hanar i en injektionsflaska (6 cm höjd och 2,5 cm i diameter) som innehåller ca 6 ml vanlig fluga mat. Flugor får lägga ägg under 12 timmar och överförs till en ny flaska på den andra dagen. 5-6 dagar efter äggläggning larver nå utfodring 3: e instar skede om upp vid 25 ° C och kan nu använ...

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<html> Den beskrivna inställning i Drosophila larver möjliggör undersökning av associativa lukt lärande inom en jämförelsevis elementära hjärna. Tillvägagångssättet är enkelt, billigt, lätt att etablera sig i ett labb och inte kräver högteknologisk utrustning 9. Vi presenterar en version av försöket, för att studera aptitskapande associativ inlärning förstärks av fruktos belöning 11. Den beskrivna inställning är baserad på en serie av parametriska studier som omfat...

<table border="1"><tbody><tr><td> Namnet av reagenset </td><td> Företag </td><td> Katalognummer </td><td> CAS-nummer </td></tr><tr><td> Fruktos </td><td> Sigma </td><td> 47.740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71.350 </td><td> 7647-14-5 </td></tr><tr><td> Agaros </td><td> Sigma </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1-oktanol </td><td> Sigma </td><td> 12.012 </td><td> 111-87-5 </td></tr><tr><td> Amylacetate </td><td> Sigma </td><td> 46.022 </td><td> 628-63-7 </td></tr><tr><td> Paraffinolja </td><td> Sigma </td><td> 18.512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

I det följande beskriver vi de metodologiska detaljer appetitive associativt lukt lärande i Drosophila larver. Upplägget i kombination med genetiska störningar, ger ett handtag för att analysera de neuronala och molekylära grunderna för specifikt associativ inlärning i en enkel larver hjärna. Organismer kan använda tidigare erfarenheter att justera nuvarande beteende. Sådant förvärv av beteende potential kan definieras som lärande och de fysiska grunderna för dessa potentialer som minne spår 1-4. Neuroforskare försöka förstå hur dessa processer är organiserade i termer av molekylära och neuronala förändringar i hjärnan med hjälp av en mängd olika metoder modellorganismer från insekter till ryggradsdjur 5,6. För sådana ansträngningar är det bra att använda modellsystem som är enkla och experimentellt tillgängliga. Drosophila larv har visat sig uppfylla dessa krav bygger påtillgången på robusta beteendemässiga analyser, förekomsten av olika transgena tekniker och det elementära organisation av nervsystemet består endast ca 10.000 neuroner (om än med vissa eftergifter: kognitiva begränsningar, några beteendemässiga alternativ och rikedom av erfarenhet tveksamt) 7-10 . Drosophila larver kan bilda föreningar mellan lukt och appetitive smak förstärkning som socker 11-14. I en vanlig analys etablerat i labbet av B. Gerber, djur får en två-lukt utbildning ömsesidig: En första grupp av larver utsätts för en lukt A tillsammans med en smak förstärkare (socker belöning) och därefter utsatt för en lukt B utan förstärkning 9. Samtidigt en andra grupp av larver får ömsesidig utbildning medan upplever lukt A utan förstärkning och därefter utsätts för lukt B med armering (socker belöning). I det följande båda grupperna är TESTed för deras önskemål mellan de två lukter. Relativt högre inställningar för belönas lukt återspeglar associativ inlärning - presenteras som en prestation (PI). Slutsatsen om associativa karaktär av prestanda index är övertygande, eftersom bortsett från oförutsedda mellan lukt och tastants, andra parametrar, såsom lukt och belöning exponering, tidens gång och hantering skiljer sig inte mellan de två grupperna 9.

Figur 1A visar en översikt av de experimentella förfaranden för larver lukt associativa inlärning. Genom att para ihop en av de presenterade två lukter med socker belöning larver förvärva beteende potential att uttrycka en attraktiv svar mot den belönade lukt i jämförelse med belönas lukt. Två grupper av larver alltid utbildade antingen kopplar ihop förstärkare med lukt oktober eller AM. Föreställningen (PI) mäter den associativa funktion som skillnaden i preferens mellan de ömsesidig...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila larver kan associera lukt stimuli smak belöning. Här beskriver vi en enkel beteendevetenskaplig paradigm som gör att analysen av appetitive associativt lukt lärande.

Appetitive Associativ Olfactory Lärande i<em&gt; Drosophila</em&gt; Larver

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

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Appetitive Associative Olfactory Learning in Drosophila Larvae

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C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

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Lokala och globala Metoder för att bedöma Thermal Nociception i<em&gt; Drosophila</em&gt; Larver

In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualisering av proprioceptorer i<em&gt; Drosophila</em&gt; Larver och puppor

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

Drosophila Vuxen Olfactory Shock Learning

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Lätt preferens analys för att studera Innate och Circadian Reglerad Photobehavior i<em&gt; Drosophila</em&gt; Larver

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Hela Mount immunomärkning av luktreceptor nervceller i<em&gt; Drosophila</em&gt; Antenn

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Elektro Metod för inspelning Intracellulärt Spännings svar av<em&gt; Drosophila</em&gt; Fotoreceptorer och Intern på ljusstimuli<em&gt; In Vivo</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

Novel analys för Cold Nociception i<em&gt; Drosophila</em&gt; Larver

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

En objektiv och reproducerbara Test av lukt lärande och diskriminering i möss

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...