Name: appetitive associative olfactory learning in drosophila larvae
Uploaded: 2013-02-18T12:00:00
Description: Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Biz özellikle deney düzeneği ve el yazması üzerinde yorumlar hakkında teknik talimatlar için Gerber laboratuar üyelerine teşekkür etmek istiyorum. Biz de vahşi tip kantonlar stoklar sinek bakım ve onarım için Lyubov Pankevych ederim. Bu çalışma DFG hibe TH1584/1-1, SNF hibe 31003A_132812 / 1 ve Konstanz Üniversitesi Zukunftskolleg (bütün AST) tarafından desteklenmektedir.

1. Hazırlık <ol><li> Drosophila vahşi tip larva 25 yetiştirilir ° C ve 14/10 ışık / karanlık döngüsü 60% -80% nem. Her zaman 20 kadın standart sinek gıda yaklaşık 6 ml içeren bir flakon (6 cm yüksekliğinde ve 2.5 cm çapında) içine 10 erkek ile konur larvaların tam yaşı kontrol etmek için. Sinekler 12 saat boyunca yumurtalarını bırakmak için izin verilir ve ikinci gününde yeni bir şişeye aktarılır. 25 ° C yetiştirilen ve hemen davranışsal deney için kullanılıp kullan?...

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<html> Drosophila larvaları anlatılan kurulum kıyaslanabilir bir ilköğretim beynin içindeki birleştirici koku öğrenme soruşturma için izin verir. Yaklaşımı bir laboratuvar kurmak için, basit, ucuz, kolay ve yüksek teknoloji ekipman 9 gerektirmez. Biz fruktoz ödül 11 ile takviye iştahlı çağrışımsal öğrenme çalışma deneyi, bir sürümü mevcut. Anlatılan kurulumdan kapsamlı eğitim denemelerinin sayısı varyasyonları, tek tahlil karşı kitle tayini, saklama ...

<table border="1"><tbody><tr><td> Reaktif Adı </td><td> Şirket </td><td> Katalog numarası </td><td> CAS numarası </td></tr><tr><td> Fruktoz </td><td> Sigma </td><td> 47740 </td><td> 57-48-7 </td></tr><tr><td> NaCl </td><td> Fluka </td><td> 71350 </td><td> 7647-14-5 </td></tr><tr><td> Agaroz </td><td> Sigma </td><td> A5093 </td><td> 9012-36-6 </td></tr><tr><td> 1-oktanol </td><td> Sigma </td><td> 12012 </td><td> 111-87-5 </td></tr><tr><td> Amylacetate </td><td> Sigma </td><td> 46022 </td><td> 628-63-7 </td></tr><tr><td> Parafin </td><td> Sigma </td><td> 18512 </td><td> 8012-95-1 </td></tr></tbody></table>

appetitive associative olfactory learning in drosophila larvae

Aşağıda biz Drosophila larvaları iştahlı çağrışımlı koku öğrenmenin metodolojik ayrıntılarını anlatacağız. Kurulumu, genetik parazit ile kombinasyon halinde basit bir larva özellikle beyinde birleştirici öğrenme nöronal ve moleküler temelleri analiz etmek için bir tutamak içerir. Organizmalar mevcut davranışlarını ayarlamak için geçmiş deneyim kullanabilirsiniz. Davranışsal potansiyeliyle ilgili bu tür satın öğrenme olarak tanımlanır ve bellek izleri 1-4 olarak bu potansiyellerin fiziksel temelleri. Edilebilir Sinir bilimciler bu süreçlerin böceklerden omurgalı 5,6 arasında değişen model organizmalarda çeşitli yöntemler kullanarak beyinde moleküler ve nöronal değişiklikleri açısından organize nasıl anlamaya çalışın. Böyle bir çaba için bu basit ve deneysel erişilebilir model sistemlerde kullanılması yardımcı olur. Drosophila larva dayalı bu talepleri karşılamak için ortaya çıktı7-10: Sağlam davranışsal deneyleri, transgenik çeşitli teknikler ve sadece yaklaşık 10.000 nöron (bilişsel sınırlamalar, birkaç davranış seçenekleri ve sorgulanabilir deneyim zenginliği bazı tavizler ile de olsa) oluşan sinir sisteminin temel örgütün varlığının durumu . Drosophila larvaları kokuları ve şeker 11-14 gibi iştahlı gustatuar donatı arasındaki ilişkileri kurabilir. Standart tahlil, B. Gerber laboratuarda kurulmuş, hayvanlar bir iki karşılıklı koku eğitim almaktadır: larva bir birinci grubu, bir tat güçlendiricidir (şeker ödül) ile bir arada bir koku maruz kalır ve daha sonra bir koku maruz kalır takviye 9 olmadan B. Takviye olmadan koku A yaşıyor ve sonradan takviye (şeker ödül) ile koku B maruz Öte yandan larva ikinci bir grup karşılıklı eğitimi alır. Aşağıda iki grup vardır tesİki kokuları arasında kendi tercihi için ted. Ödüllendirilir koku Nispeten yüksek tercihlerini çağrışımsal öğrenme yansıtır - bir performans indeksi (PI) olarak sunulmuştur. Performans indeksi ilişkisel mahiyeti ile ilgili sonuç ayrı kokular ve tastants gibi koku ve ödül pozlama, zaman ve taşıma geçişi iki grup 9 arasında farklılık yok gibi diğer parametreler arasındaki kontenjan gelen nedeni, zorlayıcı.

Şekil 1A larva olfaktör çağrışımsal öğrenme için deneysel prosedürlerin genel bir görünümünü gösterir. Bir şeker ödül larvaları ile iki sunulan kokuların bir eşleştirme ile ödüllendirilmemiş koku ile karşılaştırıldığında, ödül koku doğru çekici bir tepki ifade etmek için potansiyel davranış kazanır. Larva iki grup her zaman ya da koku Ekim veya AM ile pekiştireç eşleştirme tarafından eğitilir. Performans indeksi (Pİ) karşılıklı eğitimli gruplar aras...

Watch this Scientific Journal Video about Appetitive Associative Olfactory Learning in Drosophila Larvae at JoVE.com

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila larvaları tat ödül ile koku uyaranlara ilişkilendirmek edebiliyoruz. Burada iştahlı çağrışımlı koku öğrenme analizine olanak sağlayan bir basit davranışsal paradigma tarif.

In iştahlı İlişkilendirilebilir Olfaktör Öğrenme<em&gt; Drosophila</em&gt; Larva

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

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Appetitive Associative Olfactory Learning in Drosophila Larvae

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegans Pozitif bütanon Öğrenme, Kısa vadeli ve uzun vadeli ilişkilendirilebilir Bellek Tahliller

The memory of experiences and learned information is critical for organisms to make choices that aid their survival. C. elegans navigates its environment ...

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

Assaying Lokomotor, Öğrenme ve Hafıza Açıkları. Drosophila Nörodejenerasyon, modeller

Advances in genetic methods have enabled the study of genes involved in human neurodegenerative diseases using Drosophila as a model system1. Most of ...

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

Termal nosisepsiyon değerlendirilmesi Yerel ve Küresel Yöntemleri<em&gt; Drosophila</em&gt; Larva

In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Içinde proprioseptörlere Görselleştirmesi<em&gt; Drosophila</em&gt; Larva ve Pupa

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Drosophila Adult Olfactory Shock Learning

Drosophila Adult Olfactory Shock Learning

Drosophila Yetişkin Koku Şok Öğrenme

Drosophila have been used in classical conditioning experiments for over 40 years, thus greatly facilitating our understanding of memory, including the ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Içinde Doğuştan gelen ve sirkadiyen Ayarlı Photobehavior Eğitim ışık tercih Testi<em&gt; Drosophila</em&gt; Larva

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

In Koku Reseptör Nöronlar Tüm Dağı ile immün<em&gt; Drosophila</em&gt; Anten

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Recording Hücre içi Gerilim Yanıtları için Elektrofizyolojik Yöntem<em&gt; Drosophila</em&gt; Işık Uyaranların için Fotoreseptörler ve internöronlar<em&gt; İn Vivo</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Novel Assay for Cold Nociception in Drosophila Larvae

Novel Assay for Cold Nociception in Drosophila Larvae

Soğuk nosisepsiyon için Roman Tahlili<em&gt; Drosophila</em&gt; Larva

How organisms sense and respond to noxious temperatures is still poorly understood. Further, the mechanisms underlying sensitization of the sensory ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

Koku öğrenme ve ayrımcılık farelerde bir objektif ve tekrarlanabilir testi

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...