Name: orthotopic aortic transplantation in mice for the study of vascular disease
Uploaded: 2012-11-28T12:00:00
Description: Watch this Scientific Journal Video about Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease at JoVE.com

这项工作是由美国国立卫生研究院P30：奥布莱恩中心（DK 079337）的核心资源。

1。术前准备<ol><li>手术过程中，无论多么用心做，在显著压力的结果。为了尽量减少这种压力的影响，并最大限度地储备，动物应保持在动物饲养至少72小时之前使用10,11。 </li><li>必须是无菌的手术工具，纱布和棉签。这是没有必要使用无菌手套，提供的只是提示仪器触摸缝合或手术视野。 </li><li>用于止血，夹紧压力是非常重要的必要的最低限度。由于压力过大或粗糙处理而损坏的血?...

<ol>
	<li>Sun, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+surgical+technique+for+the+establishment+of+a+murine+model+of+aortic+transplantation.">Improved surgical technique for the establishment of a murine model of aortic transplantation.</a> Microsurgery. 18, 368-371 (1998).</li><li>Dambrin, C., Calise, D., Pieraggi, M. T., Thiers, J. C., Thomsen, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+aortic+transplantation+in+mice:+a+new+model+of+allograft+arteriosclerosis.">Orthotopic aortic transplantation in mice: a new model of allograft arteriosclerosis.</a> J. 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主动脉移植小鼠模型，因为小鼠很好定义immunogenetically 9,12,13，他们可以很容易地操纵，以改变它们的特定基因的表达，如果需要的话，提供了许多优点。正如在引进，血管外科在小鼠比大多数型号的船只的大小，因为是比较困难的。即使是伟大的动脉，如主动脉通常不超过100〜200微米内径操纵这些船只需要一个显着的技术和灵巧8,14。最常见的并发症是那些刚刚开始实施的系统观?...

<table border="1"><tbody><tr><td>项目</td><td>公司</td><td>产品编号</td></tr><tr><td>血管夹</td><td>精细科学工具</td><td> 00396-01（尺寸B-1） </td></tr><tr><td>杜蒙钳</td><td>精细科学工具</td><td> 11293-00 </td></tr><tr><td> 10-0针刺microsuture </td><td> AROSurgical </td><td> TK-107038 </td></tr><tr><td>直剪刀</td><td> Roboz手术仪器有限公司</td><td> RS-5620 </td></tr><tr><td>低温cauterizer </td><td>海狸Visitec国际</td><td> 8441000 </td></tr><tr><td>自护牵引器</td><td>世界精密仪器</td><td> 14240 </td></tr></tbody></table>

orthotopic aortic transplantation in mice for the study of vascular disease

在小鼠吻合血管手术通常被认为是困难的，高度依赖于个人的外科医生的技能。这在很大程度上是真实的，但也有一些重要的原则，可以减少这些程序的难度，并提高再现性。原位的主动脉移植是一个很好的学习过程，在这过程中这些原则，因为它涉及到两个终端到终端的吻合，但持续的成功需要有良好的缝合技术和处理的船只。这个过程开始于采购部门在收件人的原生主动脉的长度从供体动物，腹主动脉。采购主动脉收件人主动脉之间的分割端部，然后被放置到的地方使用的端至端吻合缝合。要成功实现这一目标，需要一个高集中度的好工具，稳健的手，和升值是多么容易鼠标就可以被损坏的血管，导致血栓形成。学习这些重要的原则是什么占据了大部分的初学者的时间学习显微小型啮齿动物时。在整个这个协议中，我们是指这些重要的点。该模型可用于研究血管性疾病中的各种不同的实验系统1-8。在这里示出的上下文中，它是最经常用于移植后的血管疾病，一个共同的实体器官移植的长期并发症内膜增生的发生在移植物的研究。该模型的主要优点是，它便于形态计量学分析和移植的血管位于邻接的内源性的容器，它可以作为一个附加的控制9。此处所示的技术是最经常使用小鼠体重18-25克。我们已经积累了我们的经验，使用C57BL/6J，BALB / CJ，和C3H/HeJ小鼠品系的。

图1示出了主动脉接枝。白色箭头表示的缝合线。专利接枝将显示一个可见的脉冲， 图2表示一个典型的实验中，受体存活56天的期间其次。一组由野生型（C57BL / 6×FVB）受体小鼠BALB / c小鼠主动脉移植。另一组“KO”，指定由缺乏表达血红素加氧酶1，从而导致血栓形成在24小时内移植的受惠人士半价优惠（C57BL / 6×FVB）。值得注意的是，这会导致所有收件人死亡作为图中所?...

Watch this Scientific Journal Video about Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease at JoVE.com

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

我们描述了一种技术，在该技术中的截面从一个鼠标的腹主动脉原位移植到刚好低于肾动脉在异体或同源的收件人。这种技术可能是有用的，大小均匀的大血管移植的研究中，被认为是有利的。

原位主动脉瓣移植在小鼠血管疾病的研究

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

Research

JoVE Journal

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

主动脉原位移植大鼠模型，研究慢性血管病变的发展

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

科研

医学

Orthotopic Small Bowel Transplantation in Rats

大鼠原位小肠移植

Small  bowel transplantation has become an accepted clinical option for patients with  short gut syndrome and failure of parenteral nutrition ...

Orthotopic Liver Transplantation in Rats

大鼠原位肝移植

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Orthotopic Hind Limb Transplantation in the Mouse

原位后肢移植中的鼠标

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and ...

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

主动脉钙化和炎症血管平滑肌细胞和成像的钙化

Cardiovascular disease is the leading cause of morbidity and mortality in the world.  Atherosclerotic plaques, consisting of lipid-laden macrophages and ...

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

套筒技术在主动脉移植小鼠模型中的应用-教学视频

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

Minimally Invasive Transverse Aortic Constriction in Mice

微创横向主动脉缩窄小鼠

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

球注射阿霉素对小鼠肾病综合征的诱导作用及缓释抑肽酶预防容积保留率的研究

Nephrotic syndrome is the most extreme manifestation of proteinuric kidney disease and characterized by heavy proteinuria, hypoalbuminemia, and edema due ...

A Model of Reverse Vascular Remodeling in Pulmonary Hypertension Due to Left Heart Disease by Aortic Debanding in Rats

大鼠主动脉切除术治疗左心病肺动脉高压反向血管重塑模型

Pulmonary hypertension due to left heart disease (PH-LHD) is the most common form of PH, yet its pathophysiology is poorly characterized than pulmonary ...