Name: orthotopic aortic transplantation in mice for the study of vascular disease
Uploaded: 2012-11-28T12:00:00
Description: Watch this Scientific Journal Video about Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease at JoVE.com

Dette arbejde blev finansieret af den centrale ressource af NIH P30 O&#39;Brien center (DK 079.337).

1. Presurgical Forberedelse <ol><li> Kirurgiske procedurer, uanset hvor omhyggeligt udført, resulterer i betydelig stress. For at minimere virkningerne af en sådan stress og for at maksimere reserver, bør dyrene holdes i vivarium i mindst 72 timer før brug 10,11. </li><li> Kirurgiske værktøjer, gaze, og renseservietter skal være sterilt. Det er ikke nødvendigt at anvende sterile handsker giver kun spidserne af de instrumenter touch-sutur eller den udløsende felt. </li><li> For hæmostase er det byd...

<ol>
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Musemodeller for aorta transplantation giver en række fordele, fordi mus er meget veldefineret immunogenetically 9,12,13, og de ​​let kan manipuleres til at ændre deres ekspression af specifikke gener, hvis det ønskes. Som nævnt i indledningen, karkirurgi i musen er sværere end de fleste modeller på grund af størrelsen af ​​beholderne. Selv de store arterier, såsom aorta er normalt ikke mere end 100-200 um i indre diameter, så at manipulere disse fartøjer kræver en betydelig mængde af fær...

<table border="1"><tbody><tr><td> Item </td><td> Firma </td><td> Katalog nr. </td></tr><tr><td> Vaskulære Clamps </td><td> Fin Science Tools </td><td> 00.396-01 (størrelse B-1) </td></tr><tr><td> Dumont Tang </td><td> Fin Science Tools </td><td> 11.293-00 </td></tr><tr><td> 10-0 nålet microsuture </td><td> AROSurgical </td><td> TK-107038 </td></tr><tr><td> Lige saks </td><td> Roboz Surgical Instrument Co </td><td> RS-5620 </td></tr><tr><td> Lav temperatur cauterizer </td><td> Bæver-Visitec International </td><td> 8441000 </td></tr><tr><td> Self fastholde retraktor </td><td> Verden Precision Instruments </td><td> 14.240 </td></tr></tbody></table>

orthotopic aortic transplantation in mice for the study of vascular disease

Vaskulære procedurer involverer anastomoser i musen er generelt menes at være vanskeligt og afhænger i høj grad af dygtighed af den enkelte kirurg. Det er stort set sandt, men der er en række vigtige principper, som kan reducere problemerne med disse procedurer og forbedre reproducerbarheden. Orthotopisk aorta transplantation er en fremragende procedure til at lære disse principper, fordi det involverer kun to end-to-end anastomoser, men kræver god suturering teknik og håndtering af de fartøjer, for konsekvent succes. Denne procedure begynder med tilvejebringelse af en længde af abdominal aorta fra en donor dyr, efterfulgt af opdeling af den native aorta i modtageren. Den indkøbt aorta anbringes derefter mellem de opdelte ender af modtageren aorta og sutureres på plads med end-to-end-anastomose. For at opnå dette mål med succes kræver en høj grad af koncentration, gode værktøjer, en rolig hånd, og en vurdering af, hvor let detvaskulaturen af ​​en mus kan blive beskadiget, hvilket resulterer i thrombose. Læring disse vigtige principper er, hvad fylder det meste af nybegynder tid, når læring mikrokirurgi i små gnavere. I hele denne protokol henviser vi til disse vigtige punkter. Denne model kan anvendes til at studere vaskulær sygdom i en række forskellige eksperimentelle systemer 1-8. I forbindelse vist her, er det oftest brugt til undersøgelse af efter transplantation vaskulær sygdom, en fælles langsigtet komplikation ved organtransplantation, hvor intimal hyperplasi forekommer i det transplanterede organ. Den primære fordel ved modellen er, at det letter kvantitative morfometriske analyser og det transplanterede fartøj ligger støder op til den endogene fartøj, der kan tjene som en yderligere kontrol 9. Teknikken er vist her er der oftest bruges til mus, der vejede 18-25 gram. Vi har samlet det meste af vores erfaring med at bruge C57BL/6J, BALB / cJ og C3H/HeJ stammer.

Figur 1 viser et aortaimplantatet. De hvide pile angiver suturen linjer. Et patent graft en synlig impuls. Figur 2 viser et typisk forsøg, hvor modtageren overlevelse blev fulgt i en periode på 56 dage. En gruppe bestod af vildtype (C57BL / 6 x FVB) modtagende mus transplanteret med BALB / c aorta. Den anden gruppe, betegnet &quot;KO&quot; består af modtagere (C57BL / 6 x FVB) deficiente i ekspression af hæm-oxygenase-1, hvilket resulterer i thrombose af transplantaterne inden for 2...

Watch this Scientific Journal Video about Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease at JoVE.com

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

Vi beskriver en teknik, hvor en del af den abdominale aorta fra en mus transplanteres orthotopisk til lige under de renale arterier i en allogen eller syngeniske recipient. Denne teknik kan være nyttig i undersøgelser, hvor transplantation af store arterier af ensartet størrelse er fordelagtigt.

Ortotopisk Aorta Transplantation i mus for Studiet af Vascular Disease

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

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Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

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Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

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Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

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In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and ...

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Forkalkning af vaskulære glatte muskelceller og billeddannelse af aorta Forkalkning og Inflammation

Cardiovascular disease is the leading cause of morbidity and mortality in the world.  Atherosclerotic plaques, consisting of lipid-laden macrophages and ...

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Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

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Minimalt invasiv Tværgående pulsåresammensnøring i mus

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

Induktion af Nefrotisk syndrom hos mus ved retrobulbær injektion af Doxorubicin og forebyggelse af volumen opbevaring af vedvarende frigivelse Aprotinin

Nephrotic syndrome is the most extreme manifestation of proteinuric kidney disease and characterized by heavy proteinuria, hypoalbuminemia, and edema due ...

A Model of Reverse Vascular Remodeling in Pulmonary Hypertension Due to Left Heart Disease by Aortic Debanding in Rats

En model af omvendt vaskulær ombygning i pulmonal hypertension på grund af venstre hjertesygdom ved aorta debanding hos rotter

Pulmonary hypertension due to left heart disease (PH-LHD) is the most common form of PH, yet its pathophysiology is poorly characterized than pulmonary ...