Name: cerenkov luminescence imaging (cli) for cancer therapy monitoring
Uploaded: 2012-11-13T12:00:00
Description: Watch this Scientific Journal Video about Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring at JoVE.com

我们承认由美国国家癌症研究所（NCI）R01 CA128908和斯坦福大学医学学术研究奖学金的支持。没有其他潜在的利益冲突与本文相关的报道。

1。肿瘤模型的建立<ol><li>文化H460细胞（美国典型培养物保藏中心），在补充有10％牛胎儿血清，1％青霉素/链霉素（Invitrogen公司Life Technologies公司）的RPMI 1640培养基中。应当指出的细胞株，培养基，接种的位置，数目每只小鼠的异种移植物，和其他方面的考虑，选择的都是进行调整以适应一个特定的研究的目标。在这里，我们将只提出一个具体的项目设计，作为一个例子。 </li><li>保持在5％CO <su...

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	<li>Weissleder, R., Mahmood, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+imaging.">Molecular imaging.</a> Radiology. 219 (2), 316 (2001).</li><li>Chen, K., Chen, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Positron+emission+tomography+imaging+of+cancer+biology:+current+status+and+future+prospects.">Positron emission tomography imaging of cancer biology: current status and future prospects.</a> Semin. Oncol. 38 (1), 70 (2011).</li><li>Solomon, M., Liu, Y., Berezin, M. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+in+cancer+research:+basic+principles,+tumor+detection,+and+therapeutic+monitoring.">Optical imaging in cancer research: basic principles, tumor detection, and therapeutic monitoring.</a> Med. Princ. Pract. 20 (5), 397 (2011).</li><li>Liu, H., Ren, G., Miao, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+Optical+Imaging+with+Radioactive+Probes.">Molecular Optical Imaging with Radioactive Probes.</a> PLoS One. 5 (3), e9470 (2010).</li><li>Robertson, R., Germanos, M. S., Li, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+Cerenkov+light+generation+from+positron-emitting+radiotracers.">Optical imaging of Cerenkov light generation from positron-emitting radiotracers.</a> Phys. Med. Biol. 54 (16), N355 (2009).</li><li>Xu, Y., Liu, H., Cheng, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Harnessing+the+power+of+radionuclides+for+optical+imaging:+Cerenkov+luminescence+imaging.">Harnessing the power of radionuclides for optical imaging: Cerenkov luminescence imaging.</a> J. Nucl. Med. 52 (12), 2009 (2011).</li><li>Cerenkov, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visible+emission+of+clean+liquids+by+action+of+g-radiation.">Visible emission of clean liquids by action of g-radiation.</a> Dokl Akad Nauk SSSR. 2, 451 (1934).</li><li>Cerenkov, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visible+radiation+produced+by+electrons+moving+in+a+medium+with+velocities+exceeding+that+of+light.">Visible radiation produced by electrons moving in a medium with velocities exceeding that of light.</a> Phys Rev. 52 (4), 0378 (1937).</li><li>Boschi, F., Calderan, L., D'Ambrosio, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+18F-FDG+tumour+uptake+measurements+in+small+animals+using+Cerenkov+radiation.">In vivo 18F-FDG tumour uptake measurements in small animals using Cerenkov radiation.</a> Eur. J. Nucl. Med. Mol. Imaging. 38 (1), 120 (2011).</li><li>Liu, H., Ren, G., Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+reporter+gene+expression+using+a+positron-emission-tomography+probe.">Optical imaging of reporter gene expression using a positron-emission-tomography probe.</a> J. Biomed. Opt. 15 (6), 060505 (2010).</li><li>Park, J. C., Yu, M. K., An, G. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facile+preparation+of+a+hybrid+nanoprobe+for+triple-modality+optical/PET/MR+imaging.">Facile preparation of a hybrid nanoprobe for triple-modality optical/PET/MR imaging.</a> Small. 6 (24), 2863 (2010).</li><li>Xu, Y., Chang, E., Liu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proof-of-concept+study+of+monitoring+cancer+drug+therapy+with+cerenkov+luminescence+imaging.">Proof-of-concept study of monitoring cancer drug therapy with cerenkov luminescence imaging.</a> J. Nucl. Med. 53 (2), 312 (2012).</li><li>Ellis, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bevacizumab.">Bevacizumab.</a> Nat. Rev. Drug Discov. , (2005).</li><li>Hochster, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bevacizumab+in+combination+with+chemotherapy:+first-line+treatment+of+patients+with+metastatic+colorectal+cancer.">Bevacizumab in combination with chemotherapy: first-line treatment of patients with metastatic colorectal cancer.</a> Semin. Oncol. 33, (2006).</li><li>Dothager, R. S., Goiffon, R. J., Jackson, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerenkov+radiation+energy+transfer+(CRET)+imaging:+a+novel+method+for+optical+imaging+of+PET+isotopes+in+biological+systems.">Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems.</a> PLoS One. 5 (10), e13300 (2010).</li><li>Hu, Z., Liang, J., Yang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+Cerenkov+luminescence+tomography+of+the+mouse+model+with+SPECT+imaging+validation.">Experimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation.</a> Opt. Express. 18 (24), 24441 (2010).</li><li>Park, J. C., Il An, G., Park, S. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Luminescence+imaging+using+radionuclides:+a+potential+application+in+molecular+imaging.">Luminescence imaging using radionuclides: a potential application in molecular imaging.</a> Nucl. Med. Biol. 38 (3), 321 (2011).</li><li>Holland, J. P., Normand, G., Ruggiero, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraoperative+imaging+of+positron+emission+tomographic+radiotracers+using+Cerenkov+luminescence+emissions.">Intraoperative imaging of positron emission tomographic radiotracers using Cerenkov luminescence emissions.</a> Mol. Imaging. 10 (3), 177 (2011).</li><li>Liu, H., Carpenter, C. M., Jiang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraoperative+imaging+of+tumors+using+Cerenkov+luminescence+endoscopy:+a+feasibility+experimental+study.">Intraoperative imaging of tumors using Cerenkov luminescence endoscopy: a feasibility experimental study.</a> J. Nucl. Med. , (2012).</li></ol>

CLI是一种新兴的一个有希望的分子成像技术，已经发现在许多基础科学的研究应用和临床使用4,5,15,16,17潜力。 CLI的主要优点是比传统的核医学成像方式，如PET干其使用的其他投资工具，它更容易使用，采集时间短和高吞吐量，显着更便宜，更广泛地提供给研究人员。此外，CLI除了OI一般是使用β-发光标记的分子成像探针，其中许多已通过美国食品和药物管理局（FDA），不像传统的OI剂。?...

<table border="1"&gt;<tr&gt;<td<strong&gt;名称</strong</td&gt;<td<strong&gt;公司</strong</td&gt;<td<strong&gt;产品目录号</strong</td&gt;</tr&gt;<tr&gt;<td&gt; H460细胞系</td&gt;<td&gt;美国典型培养物保藏</td&gt;<tdATCC编号：HTB-177</td&gt;</tr&gt;<tr&gt;<td&gt; RPMI 1640中等</td&gt;<tdInvitrogen生命技术</td&gt;<td&gt; 12633-012</td&gt;</tr&gt;<tr&gt;<td&gt;胎牛血清</td&gt;<tdInvitrogen生命技术</td&gt;<td&gt; 10091-148</td&gt;</tr&gt;<tr&gt;<td&gt;青霉素/链霉素</td&gt;<tdInvitrogen生命技术</td&gt;<td&gt; 15640-055</td&gt;</tr&gt;<tr&gt;<td&gt;磷酸盐缓冲盐水</td&gt;<tdInvitrogen生命技术</td&gt;<td&gt; 10010-023</td&gt;</tr&gt;<tr&gt;<td&gt;女裸鼠</td&gt;<td&gt; Charles River实验室，公司</td&gt;<td&gt;应变代号：088</td&gt;</tr&gt;<tr&gt;<td&gt;贝伐单抗（商品名Avastin）</td&gt;<td&gt;基因泰克/罗氏</td&gt;<td&gt; N / A</td&gt;</tr&gt;<tr&gt;<td&gt; MicroPET的啮齿动物R4</td&gt;<td西门子医疗解决方案（美国）公司</td&gt;<td&gt; N / A</td&gt;</tr&gt;<tr&gt;<td&gt;异氟醚（Aerrane）</td&gt;<td&gt;巴克斯特</td&gt;<td&gt;巴克斯特号码：AHN3637</td&gt;</tr&gt;<tr&gt;<td&gt; IVIS频谱</td&gt;<td卡尺生命科学</td&gt;<td&gt; N / A</td&gt;</tr&gt;</table

cerenkov luminescence imaging (cli) for cancer therapy monitoring

在分子成像，正电子发射断层扫描（PET）和光学成像（OI）的两个最重要的，从而最广泛使用的方式1-3。 PET的特点是其出色的灵敏度和定量分析能力，而OI值得注意的是无辐射，成本相对较低，扫描时间短，高吞吐量，基础研究人员和广泛的可用性。然而，这两种方式也有自己的缺点。 PET的空间分辨率差，成本高，而患有成骨不全临床前应用大多局限于有明显的散射光信号通过活组织的厚度，因为其有限的组织穿透力沿。 最近发现的切伦科夫发光成像（CLI）4-6 PET和OI之间的桥梁，又出现了。 CLI是一种新的成像方式，充分利用切伦科夫辐射（CR）图像放射性核素与其他投资工具。俄罗斯诺贝尔LAUReate阿列克谢耶维奇的切伦科夫和他的同事们最初发现于1934年CR。它是在电介质7,8在一个超光速行进时的带电粒子发射的电磁辐射的一种形式。无论是正电子或电子的带电粒子，通过移位它的原子中的电子的介质，扰动电磁场。发出的在通过中断光子是流离失所的电子返回到基态。例如，一个18 F衰变估计，以产生一个平均3光子在水5。 由于它的出现，CLI研究已在各种临床前的应用，包括在体内肿瘤的成像，报告基因显像，放射性示踪剂的发展，多模态成像，等等4,5,9,10,11供其使用。为什么CLI了巨大的成功，最重要的原因是，这项新技术利用低COST和广泛的可用性的OI图像的放射性核素，只有更昂贵和缺乏提供核成像方式，如PET成像。 在这里，我们介绍的方法使用CLI监测癌症的药物治疗。我们的研究小组最近研究这个新的应用程序，并验证了其可行性的证明了概念研究12。我们表明，CLI和PET具有优异的相关性在不同的肿瘤异种移植和成像探针。这CLI基本上是可视化的放射性核素作为PET的总体原则，CR是一致的。我们选择了贝伐单抗（商品名Avastin，基因泰克/罗氏）作为我们治疗剂，因为它是一个著名的血管生成抑制剂13,14。可以预见在不久的将来，这项技术的成熟，有一​​个显著影响临床前药物开发，筛选，以及接受治疗的患者的治疗监测。

Watch this Scientific Journal Video about Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring at JoVE.com

Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring

切伦科夫发光成像（CLI）监测的临床前癌症治疗中的使用说明在这里。这种方法利用的切伦科夫辐射（CR）和光学成像（OI）的放射性标记的探针可视化，从而提供了一种替代PET在临床前治疗的监测和药物筛选。

癌症治疗监测的切伦科夫发光成像（CLI）

In  molecular imaging, positron emission tomography (PET) and optical imaging (OI)  are two of the most important and thus most widely used modalities1-3. ...

Research

JoVE Journal

Medicine

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

生物发光和微型CT成像监测肿瘤转移和溶骨性病变

Following intracardiac delivery of MDA-MB-231-luc-D3H2LN cells to Nu/Nu mice, systemic metastases developed in the injected animals. Bioluminescence ...

科研

医学

Modeling and Imaging 3-Dimensional Collective Cell Invasion

3维建模和成像集体细胞的侵袭

A defining characteristic of cancer malignancy is invasion and metastasis 1. In some cancers (e.g. glioma 2), local invasion into surrounding healthy ...

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

协议的射频评估与互动金纳米粒子和生物系统的非侵入性癌症热疗治疗

Cancer therapies  which are less toxic and invasive than their existing counterparts are highly  desirable. The use of RF electric-fields that penetrate ...

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

肩胛间褐色脂肪组织的切伦科夫发光成像

Brown adipose tissue (BAT), widely known as a &#8220;good fat&#8221; plays pivotal roles for thermogenesis in mammals. This special tissue is closely ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

小鼠模型的非侵入性成像检测和监测卵巢癌复发

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

Dynamic Lung Tumor Tracking for Stereotactic Ablative Body Radiation Therapy

动态肺肿瘤跟踪烧蚀立体定向放射身体治疗

Physicians considering stereotactic ablative body radiation therapy (SBRT) for the treatment of extracranial cancer targets must be aware of the sizeable ...

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

一个器官高通量系统原发性多发性骨髓瘤细胞的药物敏感性的表征

In this work we describe a novel approach that combines ex vivo drug sensitivity assays and digital image analysis to estimate chemosensitivity and ...

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

结合近红外荧光成像和微计算机断层扫描直接可视化脑血栓

Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

通过轻型显微镜对小鼠急性心肌炎模型中白细胞浸润的定量可视化

Light-sheet fluorescence microscopy (LSFM), in combination with chemical clearing protocols, has become the gold standard for analyzing fluorescently ...

Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques

心脏磁共振评估疑似心脏血栓:常规和新兴技术

We present the conventional cardiac magnetic resonance (CMR) protocol for evaluating a suspected thrombus and highlight emerging techniques.&#160;The ...