Name: cerenkov luminescence imaging (cli) for cancer therapy monitoring
Uploaded: 2012-11-13T12:00:00
Description: Watch this Scientific Journal Video about Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring at JoVE.com

Vi anerkender støtte fra National Cancer Institute (NCI) R01 CA128908 og Stanford Medical Scholar Research Fellowship. Ingen anden potentiel interessekonflikt med relevans for denne artikel blev rapporteret.

1. Tumormodel <ol><li> Kultur H460-celler (American Type Culture Collection) i RPMI 1640 medium suppleret med 10% kalvefosterserum og 1% penicillin / streptomycin (Invitrogen Life Technologies). Det skal bemærkes, at valg af cellelinier, dyrkningsmedier placeringen af ​​inokulation, antal xenotransplantater per mus, og andre overvejelser er alle skal tilpasses målene i en særlig undersøgelse. Her vil vi kun præsentere ét specifikt projekt design til at tjene som en illustration. </li><li> Opretholde cellelin...

<ol>
	<li>Weissleder, R., Mahmood, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+imaging.">Molecular imaging.</a> Radiology. 219 (2), 316 (2001).</li><li>Chen, K., Chen, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Positron+emission+tomography+imaging+of+cancer+biology:+current+status+and+future+prospects.">Positron emission tomography imaging of cancer biology: current status and future prospects.</a> Semin. Oncol. 38 (1), 70 (2011).</li><li>Solomon, M., Liu, Y., Berezin, M. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+in+cancer+research:+basic+principles,+tumor+detection,+and+therapeutic+monitoring.">Optical imaging in cancer research: basic principles, tumor detection, and therapeutic monitoring.</a> Med. Princ. Pract. 20 (5), 397 (2011).</li><li>Liu, H., Ren, G., Miao, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+Optical+Imaging+with+Radioactive+Probes.">Molecular Optical Imaging with Radioactive Probes.</a> PLoS One. 5 (3), e9470 (2010).</li><li>Robertson, R., Germanos, M. S., Li, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+Cerenkov+light+generation+from+positron-emitting+radiotracers.">Optical imaging of Cerenkov light generation from positron-emitting radiotracers.</a> Phys. Med. Biol. 54 (16), N355 (2009).</li><li>Xu, Y., Liu, H., Cheng, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Harnessing+the+power+of+radionuclides+for+optical+imaging:+Cerenkov+luminescence+imaging.">Harnessing the power of radionuclides for optical imaging: Cerenkov luminescence imaging.</a> J. Nucl. Med. 52 (12), 2009 (2011).</li><li>Cerenkov, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visible+emission+of+clean+liquids+by+action+of+g-radiation.">Visible emission of clean liquids by action of g-radiation.</a> Dokl Akad Nauk SSSR. 2, 451 (1934).</li><li>Cerenkov, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visible+radiation+produced+by+electrons+moving+in+a+medium+with+velocities+exceeding+that+of+light.">Visible radiation produced by electrons moving in a medium with velocities exceeding that of light.</a> Phys Rev. 52 (4), 0378 (1937).</li><li>Boschi, F., Calderan, L., D'Ambrosio, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+18F-FDG+tumour+uptake+measurements+in+small+animals+using+Cerenkov+radiation.">In vivo 18F-FDG tumour uptake measurements in small animals using Cerenkov radiation.</a> Eur. J. Nucl. Med. Mol. Imaging. 38 (1), 120 (2011).</li><li>Liu, H., Ren, G., Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+reporter+gene+expression+using+a+positron-emission-tomography+probe.">Optical imaging of reporter gene expression using a positron-emission-tomography probe.</a> J. Biomed. Opt. 15 (6), 060505 (2010).</li><li>Park, J. C., Yu, M. K., An, G. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facile+preparation+of+a+hybrid+nanoprobe+for+triple-modality+optical/PET/MR+imaging.">Facile preparation of a hybrid nanoprobe for triple-modality optical/PET/MR imaging.</a> Small. 6 (24), 2863 (2010).</li><li>Xu, Y., Chang, E., Liu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proof-of-concept+study+of+monitoring+cancer+drug+therapy+with+cerenkov+luminescence+imaging.">Proof-of-concept study of monitoring cancer drug therapy with cerenkov luminescence imaging.</a> J. Nucl. Med. 53 (2), 312 (2012).</li><li>Ellis, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bevacizumab.">Bevacizumab.</a> Nat. Rev. Drug Discov. , (2005).</li><li>Hochster, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bevacizumab+in+combination+with+chemotherapy:+first-line+treatment+of+patients+with+metastatic+colorectal+cancer.">Bevacizumab in combination with chemotherapy: first-line treatment of patients with metastatic colorectal cancer.</a> Semin. Oncol. 33, (2006).</li><li>Dothager, R. S., Goiffon, R. J., Jackson, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerenkov+radiation+energy+transfer+(CRET)+imaging:+a+novel+method+for+optical+imaging+of+PET+isotopes+in+biological+systems.">Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems.</a> PLoS One. 5 (10), e13300 (2010).</li><li>Hu, Z., Liang, J., Yang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+Cerenkov+luminescence+tomography+of+the+mouse+model+with+SPECT+imaging+validation.">Experimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation.</a> Opt. Express. 18 (24), 24441 (2010).</li><li>Park, J. C., Il An, G., Park, S. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Luminescence+imaging+using+radionuclides:+a+potential+application+in+molecular+imaging.">Luminescence imaging using radionuclides: a potential application in molecular imaging.</a> Nucl. Med. Biol. 38 (3), 321 (2011).</li><li>Holland, J. P., Normand, G., Ruggiero, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraoperative+imaging+of+positron+emission+tomographic+radiotracers+using+Cerenkov+luminescence+emissions.">Intraoperative imaging of positron emission tomographic radiotracers using Cerenkov luminescence emissions.</a> Mol. Imaging. 10 (3), 177 (2011).</li><li>Liu, H., Carpenter, C. M., Jiang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraoperative+imaging+of+tumors+using+Cerenkov+luminescence+endoscopy:+a+feasibility+experimental+study.">Intraoperative imaging of tumors using Cerenkov luminescence endoscopy: a feasibility experimental study.</a> J. Nucl. Med. , (2012).</li></ol>

CLI fremstår som en lovende molekylær billeddannelse teknik, der har fundet potentialer i mange grundlæggende videnskab forskning applikationer og endda klinisk brug 2,3,9. De store fordele ved CLI frem for traditionelle nukleare billeddiagnostiske metoder såsom PET stammer fra brugen af ​​OI instrumenter, som er lettere at bruge, der er kendetegnet ved korte erhvervelse tid og high throughput, væsentligt billigere, og mere bredt tilgængelige for forskere. Derudover hvad adskiller CLI bortset fra OI...

<table border="1"&gt;<tr&gt;<td<strong&gt; Navn</strong</td&gt;<td<strong&gt; Firma</strong</td&gt;<td<strong&gt; Katalognummer</strong</td&gt;</tr&gt;<tr&gt;<td&gt; H460 cellelinje</td&gt;<td&gt; American Type Culture Collection</td&gt;<td&gt; ATCC nummer: HTB-177</td&gt;</tr&gt;<tr&gt;<td&gt; RPMI 1640-medium</td&gt;<td&gt; Invitrogen Life Technologies</td&gt;<td&gt; 12633-012</td&gt;</tr&gt;<tr&gt;<td&gt; Føtalt bovint serum</td&gt;<td&gt; Invitrogen Life Technologies</td&gt;<td&gt; 10091-148</td&gt;</tr&gt;<tr&gt;<td&gt; Penicillin / streptomycin</td&gt;<td&gt; Invitrogen Life Technologies</td&gt;<td&gt; 15640-055</td&gt;</tr&gt;<tr&gt;<td&gt; Phosphatpufret saltvand</td&gt;<td&gt; Invitrogen Life Technologies</td&gt;<td&gt; 10010-023</td&gt;</tr&gt;<tr&gt;<td&gt; Female athymiske nøgne mus</td&gt;<td&gt; Charles River Laboratories, Inc.</td&gt;<td&gt; Strain Code: 088</td&gt;</tr&gt;<tr&gt;<td&gt; Bevacizumab (Avastin)</td&gt;<td&gt; Genentech / Roche</td&gt;<td&gt; N / A</td&gt;</tr&gt;<tr&gt;<td&gt; MicroPET Rodent R4</td&gt;<td&gt; Siemens Medical Solutions USA, Inc.</td&gt;<td&gt; N / A</td&gt;</tr&gt;<tr&gt;<td&gt; Isofluran (Aerrane)</td&gt;<td&gt; Baxter</td&gt;<td&gt; Baxter Nummer: AHN3637</td&gt;</tr&gt;<tr&gt;<td&gt; IVIS Spectrum</td&gt;<td&gt; Caliper Life Sciences</td&gt;<td&gt; N / A</td&gt;</tr&gt;</table

cerenkov luminescence imaging (cli) for cancer therapy monitoring

I molekylær billeddannelse, er positronemissionstomografi (PET) og optisk billeddannelse (OI) to af de vigtigste og dermed mest anvendte modaliteter 1. PET er karakteriseret ved sin fremragende følsomhed og kvantificering evne mens OI er bemærkelsesværdig for ikke-stråling, relativt lave omkostninger, korte scanningstid, high throughput, og bred tilgængelighed til grundlæggende forskere. Men begge modaliteter har deres mangler så godt. PET lider af dårlig rumlig opløsning og høje omkostninger, mens OI hovedsagelig er begrænset til prækliniske anvendelser på grund af dens begrænsede vævspenetration sammen med fremtrædende spredende optiske signaler gennem tykkelsen af ​​levende væv. For nylig en bro mellem PET og OI er kommet med opdagelsen af Cerenkov Luminescence Imaging (CLI) 2-4. CLI er en ny afbildningsmodalitet der udnytter Cerenkov Radiation (CR) til billede radionuklider med OI instrumenter. Russisk Nobel laureate Alekseyevich Cerenkov og hans kolleger oprindelig opdaget CR i 1934. Det er en form for elektromagnetisk stråling, når en ladet partikel bevæger sig med en overlystunnel hastighed i et dielektrisk medium 5. Den ladede partikel, enten positron eller elektron, forstyrrer det elektromagnetiske felt af mediet ved at forskyde elektronerne i sine atomer. Efter at have passeret af afbrydelsen fotoner udsendes som de fordrevne elektroner tilbage til grundtilstanden. For eksempel blev en 18 F henfald forventes at producere et gennemsnit på 3 fotoner i vand 3. Siden sin opståen, er CLI blevet undersøgt for dens anvendelse i en række af prækliniske anvendelser, herunder in vivo-tumorafbildning, reportergen billeddannelse, radiotracer udvikling, multimodalitet billeddannelse bl.a. 2,3,6. Den vigtigste grund til, at CLI har haft stor succes indtil videre, er, at denne nye teknologi udnytter de lave omkostninger og wide tilgængeligheden af ​​OI til billede radionuklider, der anvendes, der skal afbildes kun dyrere og mindre tilgængelig nukleare billeddiagnostiske metoder såsom PET. Her præsenterer vi Metoden med at bruge CLI til at overvåge kræft medicinsk behandling. Vores gruppe har for nylig undersøgt denne nye applikation og valideret dets gennemførlighed af et proof-of-concept studie 7. Vi viste, at CLI og PET udviste fremragende sammenhænge på tværs af forskellige tumorxenotransplantater og billedbehandling sonder. Dette er i overensstemmelse med det overordnede princip om CR at CLI væsentlige visualiserer de samme radionuklider som PET. Vi valgte Bevacizumab (Avastin, Genentech / Roche) som vores terapeutisk middel, fordi det er en velkendt angiogeneseinhibitor 8. Modning af denne teknologi i den nærmeste fremtid kan forestille sig at have en betydelig indvirkning på præklinisk udvikling af lægemidler, screening, såvel som terapi overvågning af patienter, der får behandling.

Watch this Scientific Journal Video about Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring at JoVE.com

Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring

Brug af Cerenkov Luminescence Imaging (CLI) for overvågning af præklinisk kræftbehandling er beskrevet her. Denne fremgangsmåde drager fordel af Cerenkov stråling (CR) og optisk billeddannelse (OI) for at visualisere radioaktivt mærkede prober og tilvejebringer således et alternativ til PET i prækliniske terapeutisk overvågning og lægemiddelscreening.

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring

In  molecular imaging, positron emission tomography (PET) and optical imaging (OI)  are two of the most important and thus most widely used modalities1-3. ...

Research

JoVE Journal

Medicine

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Following intracardiac delivery of MDA-MB-231-luc-D3H2LN cells to Nu/Nu mice, systemic metastases developed in the injected animals. Bioluminescence ...

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Modellering og Imaging 3-Dimensional Collective Cell Invasion

A defining characteristic of cancer malignancy is invasion and metastasis 1. In some cancers (e.g. glioma 2), local invasion into surrounding healthy ...

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

Protokoller for vurdering radiofrekvens Interaktioner med Guld Nanopartikler og biologiske systemer til ikke-invasiv Hyperthermi Cancer Therapy

Cancer therapies  which are less toxic and invasive than their existing counterparts are highly  desirable. The use of RF electric-fields that penetrate ...

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Cerenkov Luminescence Imaging af interscapular brunt fedtvæv

Brown adipose tissue (BAT), widely known as a &#8220;good fat&#8221; plays pivotal roles for thermogenesis in mammals. This special tissue is closely ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Musemodel for Non-invasiv Imaging at påvise og overvåge Kræft i æggestokkene Gentagelse

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

Dynamic Lung Tumor Tracking for Stereotactic Ablative Body Radiation Therapy

Dynamisk Lung Tumor Tracking for Stereotaktisk ablative Krop strålebehandling

Physicians considering stereotactic ablative body radiation therapy (SBRT) for the treatment of extracranial cancer targets must be aware of the sizeable ...

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

En Organotypiske High Throughput System til karakterisering af Drug følsomhed Primære myelomatoseceller

In this work we describe a novel approach that combines ex vivo drug sensitivity assays and digital image analysis to estimate chemosensitivity and ...

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Kombineret Nær-infrarød Fluorescent Imaging og Micro-computertomografi for Direkte Visualisering Cerebral tromboemboli

Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Kvantitativ visualisering af leukocytinfiltrat i en murin model af Fulminant Myocarditis ved lysarkmikroskopi

Light-sheet fluorescence microscopy (LSFM), in combination with chemical clearing protocols, has become the gold standard for analyzing fluorescently ...

Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques

Hjerte magnetisk resonans til vurdering af mistanke om kardiel Trombus: konventionelle og nye teknikker

We present the conventional cardiac magnetic resonance (CMR) protocol for evaluating a suspected thrombus and highlight emerging techniques.&#160;The ...