Name: cerenkov luminescence imaging (cli) for cancer therapy monitoring
Uploaded: 2012-11-13T12:00:00
Description: Watch this Scientific Journal Video about Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring at JoVE.com

हम राष्ट्रीय कैंसर संस्थान (NCI) CA128908 R01 और स्टैनफोर्ड चिकित्सा विद्वान रिसर्च फैलोशिप से समर्थन को स्वीकार करते हैं. इस लेख के लिए प्रासंगिक रुचि का कोई अन्य संभावित संघर्ष की सूचना मिली थी.

1. ट्यूमर मॉडल <ol><li> H460 कोशिकाओं (अमेरिकी प्रकार संस्कृति संग्रह) संस्कृति में RPMI 1640 10% भ्रूण गोजातीय सीरम और 1% पेनिसिलिन / स्ट्रेप्टोमाइसिन (Invitrogen जीवन टेक्नोलॉजीज) के साथ पूरक मध्यम. यह ध्यान दिया जाना चाहिए ?...

<ol>
	<li>Weissleder, R., Mahmood, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+imaging.">Molecular imaging.</a> Radiology. 219 (2), 316 (2001).</li><li>Chen, K., Chen, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Positron+emission+tomography+imaging+of+cancer+biology:+current+status+and+future+prospects.">Positron emission tomography imaging of cancer biology: current status and future prospects.</a> Semin. Oncol. 38 (1), 70 (2011).</li><li>Solomon, M., Liu, Y., Berezin, M. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+in+cancer+research:+basic+principles,+tumor+detection,+and+therapeutic+monitoring.">Optical imaging in cancer research: basic principles, tumor detection, and therapeutic monitoring.</a> Med. Princ. Pract. 20 (5), 397 (2011).</li><li>Liu, H., Ren, G., Miao, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+Optical+Imaging+with+Radioactive+Probes.">Molecular Optical Imaging with Radioactive Probes.</a> PLoS One. 5 (3), e9470 (2010).</li><li>Robertson, R., Germanos, M. S., Li, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+Cerenkov+light+generation+from+positron-emitting+radiotracers.">Optical imaging of Cerenkov light generation from positron-emitting radiotracers.</a> Phys. Med. Biol. 54 (16), N355 (2009).</li><li>Xu, Y., Liu, H., Cheng, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Harnessing+the+power+of+radionuclides+for+optical+imaging:+Cerenkov+luminescence+imaging.">Harnessing the power of radionuclides for optical imaging: Cerenkov luminescence imaging.</a> J. Nucl. Med. 52 (12), 2009 (2011).</li><li>Cerenkov, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visible+emission+of+clean+liquids+by+action+of+g-radiation.">Visible emission of clean liquids by action of g-radiation.</a> Dokl Akad Nauk SSSR. 2, 451 (1934).</li><li>Cerenkov, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visible+radiation+produced+by+electrons+moving+in+a+medium+with+velocities+exceeding+that+of+light.">Visible radiation produced by electrons moving in a medium with velocities exceeding that of light.</a> Phys Rev. 52 (4), 0378 (1937).</li><li>Boschi, F., Calderan, L., D'Ambrosio, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+18F-FDG+tumour+uptake+measurements+in+small+animals+using+Cerenkov+radiation.">In vivo 18F-FDG tumour uptake measurements in small animals using Cerenkov radiation.</a> Eur. J. Nucl. Med. Mol. Imaging. 38 (1), 120 (2011).</li><li>Liu, H., Ren, G., Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+imaging+of+reporter+gene+expression+using+a+positron-emission-tomography+probe.">Optical imaging of reporter gene expression using a positron-emission-tomography probe.</a> J. Biomed. Opt. 15 (6), 060505 (2010).</li><li>Park, J. C., Yu, M. K., An, G. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facile+preparation+of+a+hybrid+nanoprobe+for+triple-modality+optical/PET/MR+imaging.">Facile preparation of a hybrid nanoprobe for triple-modality optical/PET/MR imaging.</a> Small. 6 (24), 2863 (2010).</li><li>Xu, Y., Chang, E., Liu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proof-of-concept+study+of+monitoring+cancer+drug+therapy+with+cerenkov+luminescence+imaging.">Proof-of-concept study of monitoring cancer drug therapy with cerenkov luminescence imaging.</a> J. Nucl. Med. 53 (2), 312 (2012).</li><li>Ellis, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bevacizumab.">Bevacizumab.</a> Nat. Rev. Drug Discov. , (2005).</li><li>Hochster, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bevacizumab+in+combination+with+chemotherapy:+first-line+treatment+of+patients+with+metastatic+colorectal+cancer.">Bevacizumab in combination with chemotherapy: first-line treatment of patients with metastatic colorectal cancer.</a> Semin. Oncol. 33, (2006).</li><li>Dothager, R. S., Goiffon, R. J., Jackson, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerenkov+radiation+energy+transfer+(CRET)+imaging:+a+novel+method+for+optical+imaging+of+PET+isotopes+in+biological+systems.">Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems.</a> PLoS One. 5 (10), e13300 (2010).</li><li>Hu, Z., Liang, J., Yang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+Cerenkov+luminescence+tomography+of+the+mouse+model+with+SPECT+imaging+validation.">Experimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation.</a> Opt. Express. 18 (24), 24441 (2010).</li><li>Park, J. C., Il An, G., Park, S. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Luminescence+imaging+using+radionuclides:+a+potential+application+in+molecular+imaging.">Luminescence imaging using radionuclides: a potential application in molecular imaging.</a> Nucl. Med. Biol. 38 (3), 321 (2011).</li><li>Holland, J. P., Normand, G., Ruggiero, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraoperative+imaging+of+positron+emission+tomographic+radiotracers+using+Cerenkov+luminescence+emissions.">Intraoperative imaging of positron emission tomographic radiotracers using Cerenkov luminescence emissions.</a> Mol. Imaging. 10 (3), 177 (2011).</li><li>Liu, H., Carpenter, C. M., Jiang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraoperative+imaging+of+tumors+using+Cerenkov+luminescence+endoscopy:+a+feasibility+experimental+study.">Intraoperative imaging of tumors using Cerenkov luminescence endoscopy: a feasibility experimental study.</a> J. Nucl. Med. , (2012).</li></ol>

CLI एक आशाजनक आणविक इमेजिंग तकनीक है कि कई बुनियादी विज्ञान अनुसंधान अनुप्रयोगों और भी नैदानिक ​​उपयोग के 2,3,9 में क्षमता पाया गया है के रूप में उभर रहा है. CLI के पारंपरिक ओय उपकरणों, जो का उपयोग करने के ?...

<table border="1"&gt;<tr&gt;<td<strong&gt; नाम</strong</td&gt;<td<strong&gt; कंपनी</strong</td&gt;<td<strong&gt; सूचीपत्र संख्या</strong</td&gt;</tr&gt;<tr&gt;<td&gt; H460 सेल लाइन</td&gt;<td&gt; अमेरिकी प्रकार संस्कृति संग्रह</td&gt;<td&gt; ATCC संख्या: HTB-177</td&gt;</tr&gt;<tr&gt;<td&gt; 1640 मध्यम RPMI</td&gt;<td&gt; Invitrogen जीवन टेक्नोलॉजीज</td&gt;<td12633-012&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; भ्रूण गोजातीय सीरम</td&gt;<td&gt; Invitrogen जीवन टेक्नोलॉजीज</td&gt;<td10091-148&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; पेनिसिलीन / स्ट्रेप्टोमाइसिन</td&gt;<td&gt; Invitrogen जीवन टेक्नोलॉजीज</td&gt;<td15640-055&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; खारा फास्फेट Buffered</td&gt;<td&gt; Invitrogen जीवन टेक्नोलॉजीज</td&gt;<td10010-023&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; महिला Athymic नग्न चूहों</td&gt;<td&gt; चार्ल्स नदी प्रयोगशालाओं इंक,</td&gt;<td&gt; तनाव कोड: 088</td&gt;</tr&gt;<tr&gt;<td&gt; Bevacizumab (Avastin)</td&gt;<td&gt; Genentech / Roche</td&gt;<td&gt; N / A</td&gt;</tr&gt;<tr&gt;<td&gt; MicroPET कृंतक R4</td&gt;<td&gt; सीमेंस चिकित्सा समाधान अमरीका, इंक,</td&gt;<td&gt; N / A</td&gt;</tr&gt;<tr&gt;<td&gt; Isoflurane (Aerrane)</td&gt;<td&gt; बैक्सटर</td&gt;<td&gt; बैक्सटर संख्या: AHN3637</td&gt;</tr&gt;<tr&gt;<td&gt; IVIS स्पेक्ट्रम</td&gt;<td&gt; कैलिपर लाइफ साइंसेज</td&gt;<td&gt; N / A</td&gt;</tr&gt;</table

cerenkov luminescence imaging (cli) for cancer therapy monitoring

In molecular imaging, positron emission tomography (PET) and optical imaging (OI) are two of the most important and thus most widely used modalities1-3. PET is characterized by its excellent sensitivity and quantification ability while OI is notable for non-radiation, relative low cost, short scanning time, high throughput, and wide availability to basic researchers. However, both modalities have their shortcomings as well. PET suffers from poor spatial resolution and high cost, while OI is mostly limited to preclinical applications because of its limited tissue penetration along with prominent scattering optical signals through the thickness of living tissues.
 Recently a bridge between PET and OI has emerged with the discovery of Cerenkov Luminescence Imaging (CLI)4-6. CLI is a new imaging modality that harnesses Cerenkov Radiation (CR) to image radionuclides with OI instruments. Russian Nobel laureate Alekseyevich Cerenkov and his colleagues originally discovered CR in 1934. It is a form of electromagnetic radiation emitted when a charged particle travels at a superluminal speed in a dielectric medium7,8. The charged particle, whether positron or electron, perturbs the electromagnetic field of the medium by displacing the electrons in its atoms. After passing of the disruption photons are emitted as the displaced electrons return to the ground state. For instance, one 18F decay was estimated to produce an average of 3 photons in water5. 
 Since its emergence, CLI has been investigated for its use in a variety of preclinical applications including in vivo tumor imaging, reporter gene imaging, radiotracer development, multimodality imaging, among others4,5,9,10,11. The most important reason why CLI has enjoyed much success so far is that this new technology takes advantage of the low cost and wide availability of OI to image radionuclides, which used to be imaged only by more expensive and less available nuclear imaging modalities such as PET.
 Here, we present the method of using CLI to monitor cancer drug therapy. Our group has recently investigated this new application and validated its feasibility by a proof-of-concept study12. We demonstrated that CLI and PET exhibited excellent correlations across different tumor xenografts and imaging probes. This is consistent with the overarching principle of CR that CLI essentially visualizes the same radionuclides as PET. We selected Bevacizumab (Avastin; Genentech/Roche) as our therapeutic agent because it is a well-known angiogenesis inhibitor13,14. Maturation of this technology in the near future can be envisioned to have a significant impact on preclinical drug development, screening, as well as therapy monitoring of patients receiving treatments.

Watch this Scientific Journal Video about Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring at JoVE.com

Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring

Cerenkov Luminescence इमेजिंग (CLI) के preclinical कैंसर के इलाज की निगरानी के लिए उपयोग वर्णित है यहाँ. इस विधि Cerenkov विकिरण (सीआर) और ऑप्टिकल इमेजिंग (ओय) radiolabeled जांच कल्पना का लाभ लेता है और इस तरह preclinical चिकित्सीय निगरानी और दवा स्क्रीनिंग में पीईटी के लिए एक विकल्प प्रदान करता है.

कैंसर के उपचार की निगरानी के लिए Cerenkov Luminescence इमेजिंग (सीएलआई)

In  molecular imaging, positron emission tomography (PET) and optical imaging (OI)  are two of the most important and thus most widely used modalities1-3. ...

Research

JoVE Journal

Medicine

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Bioluminescence और माइक्रो सीटी इमेजिंग के साथ ट्यूमर Metastases और osteolytic घावों की निगरानी

Following intracardiac delivery of MDA-MB-231-luc-D3H2LN cells to Nu/Nu mice, systemic metastases developed in the injected animals. Bioluminescence ...

Modeling and Imaging 3-Dimensional Collective Cell Invasion

मॉडलिंग और 3 - आयामी इमेजिंग कलेक्टिव सेल आक्रमण

A defining characteristic of cancer malignancy is invasion and metastasis 1. In some cancers (e.g. glioma 2), local invasion into surrounding healthy ...

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

गैर इनवेसिव अतिताप कैंसर के उपचार के लिए सोने नैनोकणों और जैविक प्रणालियों के साथ रेडियोफ्रीक्वेंसी सहभागिता मूल्यांकन के लिए प्रोटोकॉल

Cancer therapies  which are less toxic and invasive than their existing counterparts are highly  desirable. The use of RF electric-fields that penetrate ...

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Interscapular ब्राउन वसा ऊतकों के Cerenkov Luminescence इमेजिंग

Brown adipose tissue (BAT), widely known as a &#8220;good fat&#8221; plays pivotal roles for thermogenesis in mammals. This special tissue is closely ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

गैर इनवेसिव इमेजिंग के लिए murine मॉडल का पता लगाने और मॉनिटर डिम्बग्रंथि कैंसर की पुनरावृत्ति के लिए

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

Dynamic Lung Tumor Tracking for Stereotactic Ablative Body Radiation Therapy

स्टीरियोटैक्टिक पंचमी विभक्ति शरीर विकिरण थेरेपी के लिए गतिशील फेफड़े के ट्यूमर ट्रैकिंग

Physicians considering stereotactic ablative body radiation therapy (SBRT) for the treatment of extracranial cancer targets must be aware of the sizeable ...

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

प्राथमिक मल्टीपल मायलोमा प्रकोष्ठों की दवा संवेदनशीलता की विशेषता के लिए एक Organotypic उच्च Throughput सिस्टम

In this work we describe a novel approach that combines ex vivo drug sensitivity assays and digital image analysis to estimate chemosensitivity and ...

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

संयुक्त लगभग अवरक्त फ्लोरोसेंट इमेजिंग और सीधे सेरेब्रल Thromboemboli visualizing के लिए माइक्रो-गणना टोमोग्राफी

Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

लाइट शीट माइक्रोस्कोपी द्वारा फुलमिंटेंट माईकार्डाइटिस के मरीन मॉडल में ल्यूकोसाइट फैलाने का मात्रात्मक विज़ुअलाइज़ेशन

Light-sheet fluorescence microscopy (LSFM), in combination with chemical clearing protocols, has become the gold standard for analyzing fluorescently ...

Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques

संदिग्ध कार्डिएक थ्रोम्बस के मूल्यांकन के लिए कार्डिएक चुंबकीय अनुनाद: पारंपरिक और उभरते तकनीक

We present the conventional cardiac magnetic resonance (CMR) protocol for evaluating a suspected thrombus and highlight emerging techniques.&#160;The ...