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DOI :
10.3791/4356-v
November 23rd, 2012
Chapters
0:05
Title
1:24
High-throughput PCR Amplification and Purification
6:24
Preparation of Competent Cells, Cell Transformation and Antibiotic Selection
8:30
Mutant Confirmation and Storage
10:34
Results: Creation of Recombinant PCR Constructs and Correct Mutants
13:48
Conclusion
효율적인 게놈 전체의 단일 유전자 돌연변이 방법은 사용하는 설립되었습니다 연쇄상 구균 sanguinis 모델 생물로. 이 방법은 높은 처리량 재조합 PCRs 및 변환을 통해 달성했다.
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