Name: functional magnetic resonance imaging (fmri) with auditory stimulation in songbirds
Uploaded: 2013-06-03T08:56:00
Description: Watch this Scientific Journal Video about Functional Magnetic Resonance Imaging fMRI with Auditory Stimulation in Songbirds at JoVE.com

Denne forskning blev støttet af tilskud fra Research Foundation - Flandern (FWO, projekt Nr G.0420.02 og G.0443.11N), Hercules Foundation (tilskud Nr AUHA0012), samordnede forskningsaktioner (GOA finansiering) fra University of Antwerpen, og delvist sponsoreret af EC - FP6-projekt Dimi, LSHB-CT-2005 til 512.146 og EC - FP6-projekt EMIL LSHC-CT-2004 til 503.569 til A.VdL. G.DG og CP er postdocs af Research Foundation - Flandern (FWO).

1.. Forberedelse af lydstimuli <ol><li> Første optage lyd-stimuli, mens der spilles inde i boringen i 7T MR-systemet. Boringen er et lukket rum, der kan forvride de auditive stimuli resulterer i forbedring af visse auditive frekvenser. Figur 1 viser hyppigheden forbedret og undertrykt som vist ved vores optagelser af hvid støj på placeringen af fuglens hoved i magneten boring ved hjælp af en fiberoptisk mikrofon (Optimic 1160, Optoacoustics). For at kompensere denne kunstige ekstraudstyr, er en eq...

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I denne rapport beskriver vi en optimeret protokol til den detaljerede in vivo karakterisering af neurale substrater af auditiv stimulation i bedøvede zebrafinker. I overensstemmelse med den fremlagte protokol, de fleste af funktionelle hjerne aktivering hos dyr ved hjælp af BOLD fMRI bedøve dyrene under overtagelsen. Træning dyr at vænne dem til magneten miljø og scanneren støj under undersøgelsens perioder er også muligt, men snarere tidskrævende og udfordrende og derfor...

<table border="1">
 <tbody>
 <tr>
 <td>Name of the reagent/equipment</td>
 <td>Company</td>
 <td>Catalogue number</td>
 <td>Comments </td>
 </tr>
 <tr>
 <td>Isoflurane anaesthetic</td>
 <td>Isoflo</td>
 <td>05260-05</td>
 <td></td>
 </tr>
 <tr>
 <td>PC-Sam hardware/software</td>
 <td>SA-Instruments</td>
 <td></td>
 <td><a href="http://www.i4sa.com" target="_blank">http://www.i4sa.com</a></td>
 </tr>
 <tr>
 <td>Monitoring and gating system</td>
 <td></td>
 <td>1025</td>
 <td></td>
 </tr>
 <tr>
 <td>MR-compatible small rodent heater system</td>
 <td></td>
 <td></td>
 <td>Model 1025 compatible</td>
 </tr>
 <tr>
 <td>Rectal temperature probe</td>
 <td></td>
 <td>RTP-102B</td>
 <td>7'', 0.044'' </td>
 </tr>
 <tr>
 <td>7T MR scanner</td>
 <td>Bruker Biospin</td>
 <td>PHS 70/16 </td>
 <td></td>
 </tr>
 <tr>
 <td>Paravision software</td>
 <td></td>
 <td>5.1</td>
 <td></td>
 </tr>
 <tr>
 <td>Gradient Insert</td>
 <td></td>
 <td>BGA9S</td>
 <td>400 mT/m, 300A, 500V</td>
 </tr>
 <tr>
 <td>Gradient Amplifiers</td>
 <td>Copley Co., USA</td>
 <td>C256</td>
 <td></td>
 </tr>
 <tr>
 <td>Transmit resonators</td>
 <td></td>
 <td></td>
 <td>Inner diameter: 72 mm, transmit only, active decoupled</td>
 </tr>
 <tr>
 <td>Receiver antenna - 20 mm quadrature Mouse Head</td>
 <td></td>
 <td></td>
 <td>Receive only, active decoupled</td>
 </tr>
 <tr>
 <td>WaveLab software</td>
 <td>Steinberg</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Praat software</td>
 <td>Paul Boersma, University of Amsterdam</td>
 <td></td>
 <td><a href="http://www.praat.org" target="_blank">http://www.praat.org</a></td>
 </tr>
 <tr>
 <td>Non-magnetic dynamic speakers</td>
 <td>Visation, Germany</td>
 <td>HK 150</td>
 <td></td>
 </tr>
 <tr>
 <td>Fiber optic microphone</td>
 <td>Optoacoustics, </td>
 <td>Optimic 1160</td>
 <td></td>
 </tr>
 <tr>
 <td>Sound amplifier</td>
 <td>Phonic corporation</td>
 <td>MM 1002a</td>
 <td></td>
 </tr>
 <tr>
 <td>Presentation software</td>
 <td>Neurobehavioral Systems Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>MRIcro</td>
 <td>Chris Rorden</td>
 <td></td>
 <td><a href="http://www.cabiatl.com/mricro/mricro/" target="_blank">http://www.cabiatl.com/mricro/mricro/</a></td>
 </tr>
 <tr>
 <td>Statistical Parametric Mapping (SPM)</td>
 <td>Welcome Trust Centre for Neuroimaging</td>
 <td>8</td>
 <td><a href="http://www.fil.ion.ucl.ac.uk/spm/" target="_blank">http://www.fil.ion.ucl.ac.uk/spm/</a></td>
 </tr>
 </tbody>
</table>

functional magnetic resonance imaging (fmri) with auditory stimulation in songbirds

Neurobiologi af fuglesang, som en model for menneskelig tale, er en udtalt forskningsområde i adfærdsmæssige neurovidenskab. Betragtninger elektrofysiologi og molekylær tilgange tillader undersøgelse af enten forskellige stimuli om få neuroner, eller en stimulus i store dele af hjernen, blodets iltning niveau afhængig (fed) funktionel magnetisk resonans (fMRI) giver mulighed at kombinere både fordele, dvs sammenligne neurale aktivering fremkaldt af forskellige stimuli i hele hjernen på én gang. fMRI i sangfugle er udfordrende på grund af den lille størrelse af deres hjerner, og fordi deres knogler og især deres kraniet omfatter talrige lufthulrum, overtalelse vigtige modtagelighed artefakter. Gradient-ekko (GE) BOLD fMRI er blevet anvendt med succes til sangfugle 1-5 (for en gennemgang, 6. se). Disse undersøgelser fokuseret på de primære og sekundære auditive områder i hjernen, som er områder, fri for modtagelighed artefakter. Men fordi proccesser af interesse kan forekomme over disse regioner er hele hjernen BOLD fMRI kræves anvendelse af en MRI-sekvens mindre modtagelige for disse artefakter. Dette kan opnås ved hjælp af spin-ekko (SE) BOLD fMRI 7,8. I denne artikel beskriver vi, hvordan du bruger denne teknik i zebrafinker (Taeniopygia guttata), som er små sangfugle med en kropsvægt på 15-25 g omfattende undersøgt i adfærdsmæssige neurovidenskab af Fuglesang. Det vigtigste emne for fMRI undersøgelser af sangfugle er sang perception og sang læring. Den auditive karakter stimuli kombineret med den svage BOLD følsomhed SE (i forhold til GE) baserede fMRI sekvenser gør implementeringen af ​​denne teknik meget udfordrende.

<html> Vi her visuelt præsenteret en optimeret sekvens af procedurer for en vellykket billeddannelse af neurale substrater af auditive stimuli i zebra finke hjernen. For det første beskrevne procedure til fremstilling af de auditive stimuli medfører stimuli, der kan inkorporeres i en ON / OFF-blok paradigme (figur 2), og som er normaliseret til fjerne potentielle forskelle i lydtrykniveau, som kan fremkalde en differentieret reaktion i hjernen . Efter klargøring af zebra finke for MRI scanning og pla...

Watch this Scientific Journal Video about Functional Magnetic Resonance Imaging fMRI with Auditory Stimulation in Songbirds at JoVE.com

Functional Magnetic Resonance Imaging fMRI with Auditory Stimulation in Songbirds

Denne artikel viser en optimeret procedure for billeddannelse af de neurale substrater af auditiv stimulation i sangfugl hjernen hjælp af funktionel magnetisk resonans (fMRI). Det beskriver fremstillingen af ​​de sunde stimuli, positionering af emnet og erhvervelsen og den efterfølgende analyse af de fMRI data.

Funktionel magnetisk resonans (fMRI) med Auditiv Stimulation i Songbirds

The neurobiology of birdsong, as a model for human speech, is a pronounced area of research in behavioral neuroscience. Whereas electrophysiology and ...

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Functional Magnetic Resonance Imaging (fMRI) with Auditory Stimulation in Songbirds

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