Name: functional magnetic resonance imaging (fmri) with auditory stimulation in songbirds
Uploaded: 2013-06-03T08:56:00
Description: Watch this Scientific Journal Video about Functional Magnetic Resonance Imaging fMRI with Auditory Stimulation in Songbirds at JoVE.com

Denne forskningen ble støttet med tilskudd fra Research Foundation - Flandern (FWO, prosjekt Nr G.0420.02 og G.0443.11N), Hercules Foundation (tilskudd Nr AUHA0012), Samordnet Forskning Actions (GOA finansiering) fra Universitetet i Antwerpen, og delvis sponset av EU - FP6 prosjekt Dimi, LSHB-CT-2005-512146 og EC - FP6 prosjekt EMIL LSHC-CT-2004-503569 til A.VdL. G.DG og CP er postdoktorer av Research Foundation - Flandern (FWO).

En. Utarbeidelse av Auditory Stimuli <ol><li> Først registrere lyd-stimuli samtidig som spilles inne i boringen i 7T MR-systemet. Boringen er i lukkede rom som kan forvrenge auditive stimuli som resulterer i forbedring av visse auditive frekvenser. Figur 1 viser frekvensen forbedret og undertrykte som vist av våre innspillinger av hvit støy som er gjort på stedet av fuglens hode innenfor magnet fødte ved hjelp av en fiberoptisk mikrofon (Optimic 1160, Optoacoustics). For å kompensere dette nymot...

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I denne rapporten beskriver vi en optimalisert protokoll for detaljert in vivo karakterisering av nevrale substrater av auditiv stimulering i bedøvede sebra finker. I tråd med presenterte protokollen, de fleste av funksjonelle hjerneaktivitet hos dyr ved hjelp BOLD fMRI, anaesthetize dyrene under oppkjøpet. Opplæring dyr for å venne dem til magneten miljø og skanneren støy under studieopphold er også mulig, men ganske tidkrevende og utfordrende, og derfor sjelden brukt. ...

<table border="1">
 <tbody>
 <tr>
 <td>Name of the reagent/equipment</td>
 <td>Company</td>
 <td>Catalogue number</td>
 <td>Comments </td>
 </tr>
 <tr>
 <td>Isoflurane anaesthetic</td>
 <td>Isoflo</td>
 <td>05260-05</td>
 <td></td>
 </tr>
 <tr>
 <td>PC-Sam hardware/software</td>
 <td>SA-Instruments</td>
 <td></td>
 <td><a href="http://www.i4sa.com" target="_blank">http://www.i4sa.com</a></td>
 </tr>
 <tr>
 <td>Monitoring and gating system</td>
 <td></td>
 <td>1025</td>
 <td></td>
 </tr>
 <tr>
 <td>MR-compatible small rodent heater system</td>
 <td></td>
 <td></td>
 <td>Model 1025 compatible</td>
 </tr>
 <tr>
 <td>Rectal temperature probe</td>
 <td></td>
 <td>RTP-102B</td>
 <td>7'', 0.044'' </td>
 </tr>
 <tr>
 <td>7T MR scanner</td>
 <td>Bruker Biospin</td>
 <td>PHS 70/16 </td>
 <td></td>
 </tr>
 <tr>
 <td>Paravision software</td>
 <td></td>
 <td>5.1</td>
 <td></td>
 </tr>
 <tr>
 <td>Gradient Insert</td>
 <td></td>
 <td>BGA9S</td>
 <td>400 mT/m, 300A, 500V</td>
 </tr>
 <tr>
 <td>Gradient Amplifiers</td>
 <td>Copley Co., USA</td>
 <td>C256</td>
 <td></td>
 </tr>
 <tr>
 <td>Transmit resonators</td>
 <td></td>
 <td></td>
 <td>Inner diameter: 72 mm, transmit only, active decoupled</td>
 </tr>
 <tr>
 <td>Receiver antenna - 20 mm quadrature Mouse Head</td>
 <td></td>
 <td></td>
 <td>Receive only, active decoupled</td>
 </tr>
 <tr>
 <td>WaveLab software</td>
 <td>Steinberg</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Praat software</td>
 <td>Paul Boersma, University of Amsterdam</td>
 <td></td>
 <td><a href="http://www.praat.org" target="_blank">http://www.praat.org</a></td>
 </tr>
 <tr>
 <td>Non-magnetic dynamic speakers</td>
 <td>Visation, Germany</td>
 <td>HK 150</td>
 <td></td>
 </tr>
 <tr>
 <td>Fiber optic microphone</td>
 <td>Optoacoustics, </td>
 <td>Optimic 1160</td>
 <td></td>
 </tr>
 <tr>
 <td>Sound amplifier</td>
 <td>Phonic corporation</td>
 <td>MM 1002a</td>
 <td></td>
 </tr>
 <tr>
 <td>Presentation software</td>
 <td>Neurobehavioral Systems Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>MRIcro</td>
 <td>Chris Rorden</td>
 <td></td>
 <td><a href="http://www.cabiatl.com/mricro/mricro/" target="_blank">http://www.cabiatl.com/mricro/mricro/</a></td>
 </tr>
 <tr>
 <td>Statistical Parametric Mapping (SPM)</td>
 <td>Welcome Trust Centre for Neuroimaging</td>
 <td>8</td>
 <td><a href="http://www.fil.ion.ucl.ac.uk/spm/" target="_blank">http://www.fil.ion.ucl.ac.uk/spm/</a></td>
 </tr>
 </tbody>
</table>

functional magnetic resonance imaging (fmri) with auditory stimulation in songbirds

Nevrobiologi av fuglesang, som en modell for menneskelig tale, er en uttalt område av forskning i atferdsmessige nevrovitenskap. Mens elektrofysiologiske og molekylære metoder tillate etterforskningen av enten ulike stimuli på noen nevroner, eller en stimulans i store deler av hjernen, oksygentilførsel i blodet nivå avhengig (BOLD) funksjonell Magnetic Resonance Imaging (fMRI) kan kombinere både fordeler, dvs. sammenligne nevrale aktivering indusert av ulike stimuli i hele hjernen samtidig. fMRI i sangfugler er utfordrende på grunn av den lille størrelsen på sine hoder og fordi deres ben og spesielt deres skallen omfatter en rekke luft hulrom, indusere viktige mottakelighet gjenstander. Gradient-ekko (GE) BOLD fMRI har blitt brukt til sangfugler 1-5 (for en gjennomgang, se 6). Disse studiene fokuserte på de primære og sekundære auditive hjerneområder, som er områder uten mottakelighet gjenstander. Men fordi processes av interesse kan forekomme utover disse regionene, er hele hjernen BOLD fMRI nødvendig å bruke en MR-sekvens mindre utsatt for disse gjenstandene. Dette kan oppnås ved hjelp av spinn-ekko (SE) BOLD fmri 7,8. I denne artikkelen beskriver vi hvordan du bruker denne teknikken i sebra finker (Taeniopygia guttata), som er små sangfugler med en kroppsvekt på 15-25 g grundig studert i atferdsmessige nevrovitenskap av fuglesang. Hovedtema for fMRI studier på sangfugler er sang oppfatning og sang læring. Den auditive natur stimuli kombinert med svak BOLD følsomhet SE (i forhold til GE) baserte fMRI sekvenser gjør gjennomføringen av denne teknikken svært utfordrende.

<html> Vi her visuelt presenterte en optimalisert sekvens av prosedyrer for vellykket avbildning av nevrale substrater av auditive stimuli i Sebrafink hjernen. For det første, den beskrevne prosedyre for utarbeidelse av auditive stimuli fører til stimuli som kan bli innlemmet i en ON / OFF blokk paradigmet (figur 2) og som er normalisert for å eliminere potensielle forskjeller i lydtrykk som kan fremkalle en differensial respons i hjernen . Etter å forberede Sebrafink for MRI skanning og plassere den...

Watch this Scientific Journal Video about Functional Magnetic Resonance Imaging fMRI with Auditory Stimulation in Songbirds at JoVE.com

Functional Magnetic Resonance Imaging fMRI with Auditory Stimulation in Songbirds

Denne artikkelen viser en optimalisert prosedyre for avbildning av de nevrale substrater av auditiv stimulering i songbird hjernen ved hjelp av funksjonell Magnetic Resonance Imaging (fMRI). Den beskriver utarbeidelsen av lyd stimuli, plassering av faget og oppkjøp og påfølgende analyse av fMRI data.

Funksjonell Magnetic Resonance Imaging (fMRI) med auditiv stimulering i Songbirds

The neurobiology of birdsong, as a model for human speech, is a pronounced area of research in behavioral neuroscience. Whereas electrophysiology and ...

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Functional Magnetic Resonance Imaging (fMRI) with Auditory Stimulation in Songbirds

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Ruminative brooding is associated with increased vulnerability to major depression. Individuals who regularly ruminate will often try to reduce the ...

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Multifunksjonell Setup for å studere menneskelig Motor kontrollen Bruke Transkraniell magnetisk stimulering, Elektromyografi, Motion Capture, og Virtual Reality

The study of neuromuscular control of movement in humans is accomplished with numerous technologies. Non-invasive methods for investigating neuromuscular ...

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Nevrostyrt Gjentatte Transkranial magnetisk stimulering for Afasi

Repetitive transcranial magnetic stimulation (rTMS) is widely used for several neurological conditions, as it has gained acknowledgement for its potential ...