Name: the synergistic effect of visible light and gentamycin on pseudomona aeruginosa microorganisms
Uploaded: 2013-07-02T12:00:00
Description: Watch this Scientific Journal Video about The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms at JoVE.com

1。细菌培养<ol><li>革兰氏阴性的P。铜绿假单胞菌菌株PAO1菌株在37℃下在Luria肉汤（LB）中生长18小时。 </li><li>的细胞培养物，然后在7500转（发每分钟）离心分离5分钟，除去上清液。 </li><li>将细菌重新悬浮于10％LB，并重新生长另外2小时，，允许文化重新输入固定相。 </li><li>的细菌悬浮液，然后分成两组：第一组中的（2管）中加入无抗生素，在第二组中，我们添加了抗生素庆大霉素（50?...

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	<li>Feuerstein, O., Persman, N., Weiss, E. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phototoxic+Effect+of+Visible+Light+on+Porphyromonas+gingivalis+and+Fusobacterium+nucleatum:+An+In+Vitro+Study.">Phototoxic Effect of Visible Light on Porphyromonas gingivalis and Fusobacterium nucleatum: An In Vitro Study.</a> Photochemistry and Photobiology. 80, 412-415 (2004).</li><li>Enwemeka, C. S., Williams, D., Enwemeka, S. K., Hollosi, S., Yens, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blue+470-nm+light+kills+methicillin-resistant+Staphylococcus+aureus+(MRSA)+in+vitro.">Blue 470-nm light kills methicillin-resistant Staphylococcus aureus (MRSA) in vitro.</a> Photomed. Laser Surg. 27, 221-226 (2009).</li><li>Guffey, J. S., Wilborn, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+bactericidal+effects+of+405-nm+and+470-nm.">In vitro bactericidal effects of 405-nm and 470-nm.</a> Photomed. Laser Surg. 24, 684-688 (2006).</li><li>Lipovsky, A., Nitzan, Y., Friedman, H., Lubart, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sensitivity+of+Staphylococcus+aureus+strains+to+broadband+visible+light.">Sensitivity of Staphylococcus aureus strains to broadband visible light.</a> Photochem. 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L., Lilge, L., Mazzulli, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+630-,+660-,+810-,+and+905-nm+laser+irradiation+delivering+radiant+exposure+of+1-50+J/cm2+on+three+species+of+bacteria+in+vitro.">Effects of 630-, 660-, 810-, and 905-nm laser irradiation delivering radiant exposure of 1-50 J/cm2 on three species of bacteria in vitro.</a> J. Clin. Laser Med. 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光疗一直是近年来新兴的一种很有前途的方法用于治疗许多疾病在先进的多学科研究领域。在可见光范围内的光在此上下文中的使用已被广泛研究。例如，它已经发现，伤口感染是可以治愈的，更有效地让他们接触到强烈的可见光杀菌的目的。这种方法的作用机制被证明是通过感应光诱导的氧自由基（ROS），杀灭细菌11。 以往的研究已经证明亮起蓝色的光比红色和近?...

<table border="1">
 <tbody>
 <tr>
 <td>Name of the reagent</td>
 <td>Company</td>
 <td>Catalogue number</td>
 <td>Comments (optional)</td>
 </tr>
 <tr>
 <td>Lauria Broth</td>
 <td>Difco</td>
 <td>241420</td>
 <td></td>
 </tr>
 <tr>
 <td>Gentamycin</td>
 <td>Sigma </td>
 <td>G1914</td>
 <td></td>
 </tr>
 <tr>
 <td>Bacto Agar</td>
 <td>Difco </td>
 <td>231710 </td>
 <td></td>
 </tr>
 </tbody>
</table>

the synergistic effect of visible light and gentamycin on pseudomona aeruginosa microorganisms

最近有一些出版物对可见光的杀菌效果，他们大多声称，蓝色部分光谱（400纳米- 500纳米）负责杀死各种病原体1-5。建议蓝色光的光毒效应是光诱导的活性氧（ROS）的形成内源性细菌大多光敏剂吸收光线中的蓝色区域4,6,7的结果。也有红色和近红外以及绿灯9灭菌效果的报告。 在本研究中，我们开发了一种方法，使我们能够表征高功率绿色（波长为532 nm）连续（CW）和脉冲Q开关（QS）光对铜绿假单胞菌的效果。使用这种方法，我们也研究了绿灯上的细菌生存能力结合使用抗生素治疗（庆大霉素）。P.绿脓杆菌是交流OMMON noscomial条件致病菌引起的各种疾病。该菌株是相当耐各种抗生素，并包含了许多预测ACRB / MEX型RND多药外排系统10。 该方法利用固定相的自由生活的革兰阴性菌（ 铜绿假单胞菌 PAO1株），生长在Luria肉汤（LB）培养基中有和没有添加抗生素庆大霉素暴露于Q-开关和/或连续波激光器。在不同时间点测定细胞存活率。所得到的结果表明，单纯激光治疗并没有降低细胞的存活率与未处理的对照和只导致P.中的活菌数为0.5的对数减少，庆大霉素治疗绿脓杆菌 。将合并的激光和庆大霉素处理，然而，导致了协同效应和P.的可行性减少了8日志的绿脓杆菌 。 该方法可以进一步实施通过导管像设备能够注入到被感染器官的抗生素溶液同时照射光区的发展来实现。

的激光为基础的设置在图1中示意性地呈现。第一实验条件下利用连续波Nd：YAG激光具有波长532nm（第二高次谐波的Nd：YAG）和平均光功率为200 mW。此光束被分成两个光路采用光学50％/ 50％，分光镜等分裂波束，每个功率为100 mW。的光束直径约为10毫米，因此，功率密度为约100毫瓦/厘米2。曝光时间为24小时。虽然照明电源是比较高的，它没有高到足以引起加热样品。 <p class="jo...

Watch this Scientific Journal Video about The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms at JoVE.com 

The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms (Video) | JoVE 

我们表明，涉及连续的或脉冲可见激光为基础的治疗发达的生物医学装置结合使用抗生素治疗（庆大霉素），结果在统计上显着的协同效应导致的生存能力的降低<em&gt; P。铜绿</em&gt; PAO1，8日志的比较单纯抗生素治疗。

可见光和庆大霉素的协同作用<em&gt;假单胞菌假单胞菌</em&gt;微生物

Recently there were several publications on the  bactericidal effect of visible light, most of them claiming that blue part of  the spectrum (400 nm-500 ...

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Immunology and Infection

The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms

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共培养模型绿脓杆菌现场人类呼吸道细胞生长的生物膜

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