Name: the synergistic effect of visible light and gentamycin on pseudomona aeruginosa microorganisms
Uploaded: 2013-07-02T12:00:00
Description: Watch this Scientific Journal Video about The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms Video at JoVE.com

1.. Bakteriekultur <ol><li> Gramnegative P. aeruginosa stamme PAO1 blev dyrket i Luria Broth (LB) ved 37 ° C i 18 timer. </li><li> Den kultur af celler blev derefter centrifugeret ved 7.500 rpm (omdrejninger per minut) i 5 minutter og supernatanten blev fjernet. </li><li> Bakterierne blev resuspenderet i 10% LB og igen dyrket i yderligere 2 timer for at tillade kulturen at indtaste stationær fase. </li><li> Bakterierne Suspensionen blev derefter opdelt i to grupper: i den første gruppe (2 rør) ingen antib...

<ol>
	<li>Feuerstein, O., Persman, N., Weiss, E. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phototoxic+Effect+of+Visible+Light+on+Porphyromonas+gingivalis+and+Fusobacterium+nucleatum:+An+In+Vitro+Study.">Phototoxic Effect of Visible Light on Porphyromonas gingivalis and Fusobacterium nucleatum: An In Vitro Study.</a> Photochemistry and Photobiology. 80, 412-415 (2004).</li><li>Enwemeka, C. S., Williams, D., Enwemeka, S. K., Hollosi, S., Yens, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blue+470-nm+light+kills+methicillin-resistant+Staphylococcus+aureus+(MRSA)+in+vitro.">Blue 470-nm light kills methicillin-resistant Staphylococcus aureus (MRSA) in vitro.</a> Photomed. Laser Surg. 27, 221-226 (2009).</li><li>Guffey, J. 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Lysbehandling har været et felt af avanceret tværfaglig forskning i de seneste år fremstår som en lovende metode til behandling en lang række sygdomme. I denne forbindelse anvendelsen af ​​lys i det synlige område er blevet grundigt undersøgt. For eksempel har det vist sig, inficerede sår kan blive helbredt mere effektivt ved at udsætte dem for intens synligt lys for sterilisering. Virkningsmekanismen for denne fremgangsmåde viste sig at være gennem induktionen af lys-inducerede oxygenradikaler (ROS), som...

<table border="1">
 <tbody>
 <tr>
 <td>Name of the reagent</td>
 <td>Company</td>
 <td>Catalogue number</td>
 <td>Comments (optional)</td>
 </tr>
 <tr>
 <td>Lauria Broth</td>
 <td>Difco</td>
 <td>241420</td>
 <td></td>
 </tr>
 <tr>
 <td>Gentamycin</td>
 <td>Sigma </td>
 <td>G1914</td>
 <td></td>
 </tr>
 <tr>
 <td>Bacto Agar</td>
 <td>Difco </td>
 <td>231710 </td>
 <td></td>
 </tr>
 </tbody>
</table>

the synergistic effect of visible light and gentamycin on pseudomona aeruginosa microorganisms

For nylig var der en række publikationer om baktericide effekt af synligt lys, de fleste af dem hævder, at blå del af spektret (400 nm-500 nm) er ansvarlig for at dræbe forskellige patogener 1-5. Den fototoksiske effekt af blåt lys blev foreslået at være et resultat af lys-inducerede reaktive oxygenarter (ROS) dannelse af endogene bakterielle fotosensibilisatorer som hovedsagelig absorberer lys i det blå område 4,6,7. Der er også rapporter om biocid virkning af røde og nærinfrarøde 8 såvel som grønt lys 9.. I den foreliggende undersøgelse har vi udviklet en metode, der tillod os at karakterisere virkningen af høj effekt grøn (bølgelængde på 532 nm) kontinuerlig (CW) og pulset Q-switched (QS) lys på Pseudomonas aeruginosa. Ved hjælp af denne metode, vi også studeret effekten af grønne lys kombineret med antibiotisk behandling (gentamycin) på bakterierne levedygtighed. P. aeruginosa er acFÆLLES noscomial opportunistisk patogen forårsager forskellige sygdomme. Stammen er forholdsvis modstandsdygtig over for forskellige antibiotika, og indeholder mange forudsagde AcrB / Mex-typen RND multistofudstrømningspumpen systemer 10.. Den anvendte metode fritlevende stationær fase Gram-negative bakterier (P. aeruginosa stamme PAO1), dyrket i Luria Broth (LB) medium udsættes for Q-switched og / eller CW-lasere med og uden tilsætning af antibiotikummet gentamycin. Cellelevedygtighed blev bestemt på forskellige tidspunkter. De opnåede resultater viser, at laserbehandling alene ikke reducerer cellelevedygtigheden sammenlignet med ubehandlet kontrol, og at gentamycin behandling alene kun resulterede i en 0,5 log reduktion i kimtal for P. aeruginosa. Den kombinerede laser og gentamycin behandling dog resulterede i en synergistisk effekt og levedygtigheden af P. aeruginosa blev reduceret med 8 stammens. Den foreslåede metode kan endvidere være gennemmenteret gennem udvikling af kateteret lignende anordning, der kan injicere et antibiotikum løsning i den inficerede orgel samtidig belyse området med lys.

Den laserbaseret opsætning er skematisk vist i figur 1.. Den første eksperimentelle betingelse udnyttede en CW Nd: YAG laser med bølgelængde på 532 nm (den anden harmoniske af Nd: YAG) og gennemsnitlig optisk effekt på 200 mW. Denne stråle blev opdelt i to optiske veje bruger optisk 50% / 50% beam splitter sådan, at hver split stråle havde effekt på 100 mW. Strålediameteren var omkring 10 mm, og dermed effekttæthed var omkring 100 mW / cm 2. Eksponeringen varede 24 timer. Selvom b...

Watch this Scientific Journal Video about The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms Video at JoVE.com

The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms Video (Video) | JoVE

Vi viser, at et udviklet biomedicinske indretning involverer kontinuerlig eller pulserende synlig laser baseret behandling, der er kombineret med antibiotisk behandling (gentamycin), resulterer i en statistisk signifikant synergistisk virkning fører til en reduktion i levedygtighed<em&gt; P. aeruginosa</em&gt; PAO1, med 8 stammens forhold til antibiotisk behandling alene.

Den synergistiske virkning af synligt lys og Gentamycin på<em&gt; Pseudomona aeruginosa</em&gt; Mikroorganismer

Recently there were several publications on the  bactericidal effect of visible light, most of them claiming that blue part of  the spectrum (400 nm-500 ...

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The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms

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