Name: surgical technique for spinal cord delivery of therapies: demonstration of procedure in gottingen minipigs
Uploaded: 2012-12-07T12:00:00
Description: Watch this Scientific Journal Video about Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs at JoVE.com

我们感谢埃默里大学部兽医照顾动物资源的工作人员。资金来源包括：ALS协会，国防部，Neuralstem公司

1。动物用已批准程序演示本文由埃默里大学实验动物护理和使用委员会（IACUC）。女哥廷根小型猪，体重约15-20公斤。 2。麻醉动物约12小时，在手术前禁食。动物镇静和麻醉诱导肌注氯氨酮（35毫克/千克），乙酰丙嗪（1.1毫克/公斤），与阿托品（0.04毫克/千克）的鸡尾酒。动物的氧和1-3％的异氟醚全身麻醉气管插管和维护。在这一点上...

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	<li>Usvald, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+dosing+regimen+and+reproducibility+of+intraspinal+grafting+of+human+spinal+stem+cells+in+immunosuppressed+minipigs.">Analysis of dosing regimen and reproducibility of intraspinal grafting of human spinal stem cells in immunosuppressed minipigs.</a> Cell Transplant. 19, 1103-1122 (2010).</li><li>Riley, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+spinal+cord+therapeutics+delivery:+stabilized+platform+and+microelectrode+recording+guidance+validation.">Targeted spinal cord therapeutics delivery: stabilized platform and microelectrode recording guidance validation.</a> Stereotact. Funct. Neurosurg. 86, 67-74 (2008).</li><li>Federici, T., Riley, J., Park, J., Bain, M., Boulis, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preclinical+safety+validation+of+a+stabilized+viral+vector+direct+injection+approach+to+the+cervical+spinal+cord.">Preclinical safety validation of a stabilized viral vector direct injection approach to the cervical spinal cord.</a> Clin. Transl. Sci. 2, 165-167 (2009).</li><li>Riley, J. P., Raore, B., Taub, J. S., Federici, T., Boulis, N. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Platform+and+Cannula+Design+Improvements+for+Spinal+Cord+Therapeutics+Delivery.">Platform and Cannula Design Improvements for Spinal Cord Therapeutics Delivery.</a> Neurosurgery. 69, 147-154 (2011).</li><li>Riley, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cervical+spinal+cord+therapeutics+delivery:+preclinical+safety+validation+of+a+stabilized+microinjection+platform.">Cervical spinal cord therapeutics delivery: preclinical safety validation of a stabilized microinjection platform.</a> Neurosurgery. 65, 754-761 (2009).</li><li>Raore, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cervical+multilevel+intraspinal+stem+cell+therapy:+assessment+of+surgical+risks+in+Gottingen+minipigs.">Cervical multilevel intraspinal stem cell therapy: assessment of surgical risks in Gottingen minipigs.</a> Spine. 36, 164-171 (2011).</li><li>Boulis, N. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translational+stem+cell+therapy+for+amyotrophic+lateral+sclerosis.">Translational stem cell therapy for amyotrophic lateral sclerosis.</a> Nat. Rev. Neurol. , (2011).</li><li>Riley, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraspinal+Stem+Cell+Transplantation+in+ALS:+A+Phase+I+Safety+Trial,+Technical+Note+&#38;+Interim+Safety+Outcomes.">Intraspinal Stem Cell Transplantation in ALS: A Phase I Safety Trial, Technical Note &#38; Interim Safety Outcomes.</a> Neurosurgery. 71 (2), 405-416 (2012).</li><li>Boulis, N. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lumbar+intraspinal+injection+of+neural+stem+cells+in+patients+with+ALS:+results+of+a+Phase+I+trial+in+12+patients.">Lumbar intraspinal injection of neural stem cells in patients with ALS: results of a Phase I trial in 12 patients.</a> Stem Cells. 30 (6), 1144-1151 (2012).</li></ol>

尽管人类的7-9所描述的技术进行批准，为了脊髓治疗成功的关键问题仍有待回答。 脊髓耐受性脑实质内注射需要一个严格的理解，使开发疗法的试验性脱髓鞘性，变性，外伤性脊髓病的规划和执行。目前，还没有清晰的认识注射的数量，大型哺乳动物的脊髓可以承受的，没有暂时性和永久性的发病率。同样，注射的间隔可能是影响发病率。此外，宏观的（ 例如 ，通风相关?...

<table border="1"><tbody><tr><td> 的设备名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td>脊柱德里克 - </td><td> Neuralstem公司</td><td></td><td> Neuralstem，公司已经购买了这项技术的独家授权</td></tr><tr><td> MINJ-PD微量注射器泵</td><td> ，Tritech公司研究公司</td><td></td><td>这个特定的应用程序定制</td></tr><tr><td>浮动套管</td><td> Neuralstem公司</td><td></td><td>自定义设计的这个特定的应用程序的</td></tr></tbody></table>

surgical technique for spinal cord delivery of therapies: demonstration of procedure in gottingen minipigs

这是一个紧凑的可视描述的手术技术和装置，用于提供到脊髓（基因和细胞）疗法的组合。虽然该技术被证明在动物体内，该过程是FDA批准的，目前用于ALS患者的脊髓干细胞移植到。虽然FDA已确认疗效高度特征的啮齿动物模型中证明的原则上的数据，大型动物的使用被认为是关键的，用于验证的组合的外科手术过程中，移动设备，和供人类使用的安全性的最终治疗。的大小，解剖学和一般的脆弱性脊椎和脊椎神经的猪被确认为更好的模型的人。而且从人类的揭露和处理以及封闭伤口在猪脊髓，手术过程中几乎没有区别。我们相信，健康的猪模型是一个关键校准第一步在研究程序安全。

<table border="1"><tbody><tr><td colspan="2"> Tarlov评分</td></tr><tr><td> 0 </td><td>瘫痪，无运动</td></tr><tr><td> 1 </td><td>能感觉到的强直性在后肢，轻微的运动</td></tr><tr><td> 2 </td><td>在后肢的运动，但不能坐或站</td></tr><tr><td> 3 </td><td>站立和行走的能力，但共济失调，短期内</td></tr><tr><td> 4 </td><td>完全恢复，运动功能正常; </td></tr></tbody></table> ?...

Watch this Scientific Journal Video about Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs at JoVE.com

Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs

短期视觉描述的手术技术和设备用于交付（基因和细胞）治疗脊髓。中的动物，但该技术被证明是完全平移和当前正用于人类应用。

外科技术治疗脊髓交付：在哥廷根小型猪演示程序

This is a  compact visual description of a combination of surgical technique and device  for the  delivery of (gene and cell) therapies into the spinal ...

Research

JoVE Journal

Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

在大鼠脊髓损伤后的急性和慢性触觉测试

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

科研

医学

A Contusion Model of Severe Spinal Cord Injury in Rats

挫伤模型脊髓严重损伤的保护作用

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

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控制皮层影响模型创伤性脑损伤

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Minimal Invasive Surgical Procedure of Inducing Myocardial Infarction in Mice

在诱导小鼠心肌梗死的微创手术过程

Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last ...

Calibrated Forceps Model of Spinal Cord Compression Injury

脊髓压迫损伤的校准钳型

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...

Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury

超声造影成像脊髓血流量的评估实验脊髓损伤

Reduced spinal cord blood flow (SCBF) (i.e., ischemia) plays a key role in traumatic spinal cord injury (SCI) pathophysiology and is accordingly an ...

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

老鼠圆窗途径耳毒性剂递送：一个快速和可靠的技术诱导耳蜗细胞变性

Investigators have utilized a wide array of animal models and investigative techniques to study the mammalian auditory system. Much of the basic research ...

A Neonatal Mouse Spinal Cord Compression Injury Model

新生儿小鼠脊髓压迫损伤模型

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

大鼠心肌缺血再灌注后冠状动脉流动储备评价

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...