Name: isolation, characterization and comparative differentiation of human dental pulp stem cells derived from permanent teeth by using two different methods
Uploaded: 2012-11-24T14:00:00
Description: Watch this Scientific Journal Video about Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods at JoV

Vi takknemlig erkjenne Dr. Leila Shakeri &amp; Dr. Aref Dournaei for sin kritiske diskos og Mr. Mohammad Reza Khadem Sharif for hans tekniske støtte.

1. Forbered Enzyme Solution og spredning Medium (PM) <ol><li> Merke collagenase Type I Løsning: Vei opp kollagenase type I (12 mg / ml) og oppløses i 1 ml PBS og filteret med en 0,2 um sprøytefilter. Deretter plasserer den 15 ml tube og holde det ved -20 ° C inntil nødvendig. </li><li> Merke dispase Løsning: Vei opp dispase (16 mg / ml) og oppløses i 1 ml PBS og filteret med en 0,2 um sprøytefilter. Deretter plasserer den 15 ml tube og holde den ved 4 ° C inntil nødvendig. <...

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	<li>Volponi, A. A., Pang, Y., Sharpe, P. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stem+cell-based+biological+tooth+repair+and+regeneration.">Stem cell-based biological tooth repair and regeneration.</a> Trends Cell Biol. 20, 715-722 (2010).</li><li>Nosrat, I. V., Widenfalk, J., Olson, L., Nosrat, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dental+pulp+cells+produce+neurotrophic+factors,+interact+with+trigeminal+neurons+in+vitro,+and+rescue+motoneurons+after+spinal+cord+injury.">Dental pulp cells produce neurotrophic factors, interact with trigeminal neurons in vitro, and rescue motoneurons after spinal cord injury.</a> Dev. Biol. 238, 120-132 (2001).</li><li>Gandia, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+dental+pulp+stem+cells+improve+left+ventricular+function,+induce+angiogenesis,+and+reduce+infarct+size+in+rats+with+acute+myocardial+infarction.">Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction.</a> Stem Cells. 26, 638-645 (2008).</li><li>Gronthos, S., Mankani, M., Brahim, J., Robey, P. G., Shi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postnatal+human+dental+pulp+stem+cells+(DPSCs)+in+vitro+and+in+vivo.">Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.</a> Proc. Natl. Acad. Sci. U. S. 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Endod. 34 (08), 962-969 (2008).</li><li>Pierdomenico, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multipotent+mesenchymal+stem+cells+with+immunosuppressive+activity+can+be+easily+isolated+from+dental+pulp.">Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp.</a> Transplantation. 80, 836-842 (2005).</li><li>de Mendonca Costa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reconstruction+of+large+cranial+defects+in+nonimmunosuppressed+experimental+design+with+human+dental+pulp+stem+cells.">Reconstruction of large cranial defects in nonimmunosuppressed experimental design with human dental pulp stem cells.</a> J. Craniofac. Surg. 19, 204-210 (2008).</li><li>Tirino, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methods+for+the+identification,+characterization+and+banking+of+human+DPSCs:+current+strategies+and+perspectives.">Methods for the identification, characterization and banking of human DPSCs: current strategies and perspectives.</a> Stem Cell Rev. 7, 608-615 (2011).</li><li>Tirino, V., Paino, F., De Rosa, A., Papaccio, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification,+isolation,+characterization,+and+banking+of+human+dental+pulp+stem+cells.">Identification, isolation, characterization, and banking of human dental pulp stem cells.</a> Methods Mol. Biol. 879, 443-463 (2012).</li><li>Eslaminejad, M. B., Vahabi, S., Shariati, M., Nazarian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+Growth+and+Characterization+of+Stem+Cells+from+Human+Dental+Pulp+of+Deciduous+Versus+Permanent+Teeth.">In vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth.</a> J. Dent. (Tehran). 7, 185-195 (2010).</li><li>Wei, X., Ling, J., Wu, L., Liu, L., Xiao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+mineralization+markers+in+dental+pulp+cells.">Expression of mineralization markers in dental pulp cells.</a> J. Endod. 33, 703-708 (2007).</li><li>Nombela-Arrieta, C., Ritz, J., Silberstein, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+elusive+nature+and+function+of+mesenchymal+stem+cells.">The elusive nature and function of mesenchymal stem cells.</a> Nat. Rev. Mol Cell Biol. 12, 126-131 (2011).</li><li>Huang, G. T., Gronthos, S., Shi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+stem+cells+derived+from+dental+tissues+vs.+those+from+other+sources:+their+biology+and+role+in+regenerative+medicine.">Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine.</a> J. Dent. Res. 88, 792-806 (2009).</li><li>Graziano, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Scaffold's+surface+geometry+significantly+affects+human+stem+cell+bone+tissue+engineering.">Scaffold's surface geometry significantly affects human stem cell bone tissue engineering.</a> J. Cell Physiol. 214, 166-172 (2008).</li><li>d'Aquino, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+dental+pulp+stem+cells:+from+biology+to+clinical+applications.">Human dental pulp stem cells: from biology to clinical applications.</a> J. Exp. Zool. B. Mol. Dev. Evol. 312, 408-415 (2009).</li><li>Mitsiadis, T. A., Feki, A., Papaccio, G., Caton, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dental+pulp+stem+cells,+niches,+and+notch+signaling+in+tooth+injury.">Dental pulp stem cells, niches, and notch signaling in tooth injury.</a> Adv. Dent. Res. 23, 275-279 (2011).</li><li>Shi, S., Gronthos, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perivascular+niche+of+postnatal+mesenchymal+stem+cells+in+human+bone+marrow+and+dental+pulp.">Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp.</a> J. Bone Miner. Res. 18, 696-704 (2003).</li><li>Tomic, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunomodulatory+properties+of+mesenchymal+stem+cells+derived+from+dental+pulp+and+dental+follicle+are+susceptible+to+activation+by+toll-like+receptor+agonists.">Immunomodulatory properties of mesenchymal stem cells derived from dental pulp and dental follicle are susceptible to activation by toll-like receptor agonists.</a> Stem Cells Dev. 20, 695-708 (2011).</li><li>Tsukamoto, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mineralized+nodule+formation+by+cultures+of+human+dental+pulp-derived+fibroblasts.">Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts.</a> Arch. Oral Biol. 37, 1045-1055 (1992).</li><li>About, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+dentin+production+in+vitro.">Human dentin production in vitro.</a> Exp. Cell Res. 258, 33-41 (2000).</li><li>Couble, M. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odontoblast+differentiation+of+human+dental+pulp+cells+in+explant+cultures.">Odontoblast differentiation of human dental pulp cells in explant cultures.</a> Calcif. Tissue Int. 66, 129-138 (2000).</li><li>Onishi, T., Kinoshita, S., Shintani, S., Sobue, S., Ooshima, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stimulation+of+proliferation+and+differentiation+of+dog+dental+pulp+cells+in+serum-free+culture+medium+by+insulin-like+growth+factor.">Stimulation of proliferation and differentiation of dog dental pulp cells in serum-free culture medium by insulin-like growth factor.</a> Arch. Oral Biol. 44 (99), 361-371 (1999).</li><li>Huang, G. T., Sonoyama, W., Chen, J., Park, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+characterization+of+human+dental+pulp+cells:+various+isolation+methods+and+culturing+environments.">In vitro characterization of human dental pulp cells: various isolation methods and culturing environments.</a> Cell Tissue Res. 324, 225-236 (2006).</li><li>Bakopoulou, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+the+impact+of+two+different+isolation+methods+on+the+osteo/odontogenic+differentiation+potential+of+human+dental+stem+cells+derived+from+deciduous+teeth.">Assessment of the impact of two different isolation methods on the osteo/odontogenic differentiation potential of human dental stem cells derived from deciduous teeth.</a> Calcif. Tissue Int. 88, 130-141 (2011).</li><li>Curtis, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=EF1alpha+and+RPL13a+represent+normalization+genes+suitable+for+RT-qPCR+analysis+of+bone+marrow+derived+mesenchymal+stem+cells.">EF1alpha and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells.</a> BMC Mol. Biol. 11, 61 (2010).</li><li>Suchanek, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dental+pulp+stem+cells+and+their+characterization.">Dental pulp stem cells and their characterization.</a> Biomed. Pap. Med. Fac. Univ. 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Denne protokollen beskriver prosessen med isolering og karakterisering av hDPSCs fra tannpulpa ved hjelp av to metoder, enzymatisk dissosiasjon og direkte resultat av stamceller fra pulpavevet explants. I tillegg, i vitro differensiering av disse cellene til odontoblaster, ble vurdert av kvantitativ Alizarin Red S analysen &amp; QPCR. Eksisterende protokoller for å isolere pulpavevet fra menneskelig tann hadde blitt brukt ulike instrumenter som tang (bein tang) 9, utryddelsen ...

Stamceller er clonogenic celler som besitter to bemerkelsesverdige funksjoner, kjent som multi-styrke og selvfornyelse 15. Blant alle stamceller med ulike replicative potencies, har dental stamceller som de postnatal stamceller trukket oppmerksomhet de siste årene på grunn av sin tilgjengelighet, plastisitet, og høy proliferativ evne sammenlignet med andre voksne stamceller 16. Karakteristisk, ligner på stamceller, dental pulp stamceller er adherente clonogenic celler som har multippel differensiering kapasitet inn mesenchyme og / eller ikke-mesenchyme linjene, både in vitro og in vivo. 1-8,17,18 DPSCs identifiseres ved deres negative uttrykk for blodkreft antigener (f.eks CD45, CD34, CD14), og positive uttrykk for CD90, CD29, CD73, CD105, CD44 og STRO-1. 19,20 Enkel innhentet potensial med minimum smerte og sykelighet gjør menneskelige DPSCs som verdifullestand kilde MSCS forhold til ordinær kilder, for eksempel benmarg stamceller 21. Generelt har DPSCs blitt isolert ved enten utvekst metode, dvs. migrering av stamceller fra pulpavevet eksplanter (DPSC-OG) 22-24, og / eller ved enzymatisk fordøyelse (DPSC-ED) 4,6,25. Tidligere studier har vist at den isolasjon metoden og dyrkningsbetingelser kan indusere forskjellige populasjoner eller linjene under i vitro passasjer 26,27. I tilfelle av permanente tenner (pDPSCs), avslørte Huang et al at enzymatiske fordøyd pDPSCs har høyere spredning potensiale sammenlignet med de vokst DPSCs. 26 Videre, i tilfelle av melketenner (dDPSCs), ble det vist at STRO-1 &amp; CD34 markører uttrykkes mer i dDPSC-ED sammenlignet med dDPSC-OG. I tillegg, viste dDPSC-ED høyere mineralisering rate i definerte osteo / odonto medium. 27 Derfor, på grunn av den enestående potensialet DPSCs i regenerative medisin, vil flere studier være nødvendig for bedre forståelse av mulige ulike populasjoner som er utledet fra ulike isolasjon metoder. Her var det forsøk på å innføre enkel måte papirmasse ekstraksjon, ved å bruke en-trinns dental diamant disk å forenkle prosessen med papirmasse utvinning. Dessuten, etter isolering av humane papirmasse-avledede stamceller ved å bruke ED eller OG metoder ble generelle egenskaper og differensiering kapasitet mellom to grupper også undersøkt.

<table border="1"><tbody><tr><td> Navnet på reagensen </td><td> Selskapet </td><td> Katalog eller Lot. nummer </td><td> Kommentarer (valgfritt) </td></tr><tr><td> α-MEM </td><td> GIBCO </td><td> 11900-073 </td><td></td></tr><tr><td> Kollagenase type I </td><td> Sigma-Aldrich </td><td> C0130-100mg </td><td></td></tr><tr><td> Dispase </td><td> GIBCO </td><td> 17105-041 </td><td></td></tr><tr><td> Penicillin / streptomycin </td><td> GIBCO </td><td> 15140-122 </td><td></td></tr><tr><td> Amfotericin B </td><td> GIBCO </td><td> 15290-018 </td><td></td></tr><tr><td> Føtalt bovint serum definert (FBS) </td><td> HyClone </td><td> SH30070.03 </td><td></td></tr><tr><td> L-askorbinsyre 2-fosfat </td><td> Sigma </td><td> A8960-5G </td><td></td></tr><tr><td> L-glutamin </td><td> GIBCO </td><td> 25030-024 </td><td></td></tr><tr><td> Deksametason </td><td> Sigma </td><td> D4902 </td><td></td></tr><tr><td> β-glycerol fosfat disodium salt hydrate, BioUltra </td><td> Sigma </td><td> G9422-100G </td><td></td></tr><tr><td> Kalium monobasisk </td><td> Sigma-Aldrich </td><td> P5655 </td><td></td></tr><tr><td> Osteogenesis Assay Kit </td><td> Millipore </td><td> PS01802031 </td><td></td></tr><tr><td> Mus IgG2b-PE isotype kontroll </td><td> BD pharmingen </td><td> 50808088029 </td><td></td></tr><tr><td> FITC mus IgG2b isotype kontroll </td><td> BD pharmingen </td><td> 559532 </td><td></td></tr><tr><td> FITC mus IgG1 κ isotype </td><td> BD pharmingen </td><td> 11471471 </td><td></td></tr><tr><td> FITC / PE mus anti-human CD34/CD45 </td><td> BD pharmingen </td><td>341071 </td><td></td></tr><tr><td> PE anti-human CD146 </td><td> BD pharmingen </td><td> 550315 </td><td></td></tr><tr><td> Monoklonalt mus anti-human CD90/FITC </td><td> Daka </td><td> 00034418 </td><td></td></tr><tr><td> PE mus anti-human CD73 </td><td> BD pharmingen </td><td> 550257 </td><td></td></tr><tr><td> Anti-h CD105/Endoglin PE </td><td> BD pharmingen </td><td> FAB10971P </td><td></td></tr><tr><td> PE mus anti-human CD11b/Mac1 </td><td> BD pharmingen </td><td> 5553888 </td><td></td></tr><tr><td> CD44 PE mus anti menneskelig </td><td> BD pharmingen </td><td> 555479 </td><td></td></tr><tr><td> Fosfatbufferoppløsning (PBS) </td><td> GIBCO </td><td> 003000 </td><td></td></tr><tr><td> 70-mikrometer celle sil </td><td> Falk </td><td> 352360 </td><td></td></tr><tr><td&gt; 0,2 mikrometer sprøyte filter </td><td> Millex-GV </td><td> SLGV033RB </td><td></td></tr><tr><td> 25 cm 2 kultur kolbe </td><td> Sigma-Aldrich </td><td> Z707481 </td><td></td></tr><tr><td></td><td></td><td></td><td> UTSTYR </td></tr><tr><td> BD FACSCalibur </td><td> BD </td><td> 342975 </td><td></td></tr><tr><td> multiskan mikroplate spektrofotometer </td><td> Thermo Scientific </td><td> 51119200 </td><td></td></tr><tr><td> Fleurcense Microscope </td><td> Olympus </td><td></td><td></td></tr><tr><td> Flowing Software versjon 2.3.1 </td><td></td><td></td><td></td></tr></tbody></table>

isolation, characterization and comparative differentiation of human dental pulp stem cells derived from permanent teeth by using two different methods

<p class="jove_content"&gt; Utvikle visdomstennene er enkle tilgjengelige kilde til stamceller under voksenlivet som kunne oppnås ved rutinemessige kjeveortopedisk behandling. Menneskelige pulp-avledet stamceller (hDPSCs) har høy spredning potensial med multi-avstamning differensiering kapasitet i forhold til den ordinære kilden voksne stamceller<sup&gt; 1-8</sup&gt;, Og derfor kan hDPSCs være gode kandidater for autolog transplantasjon i tissue engineering og regenerativ medisin. Sammen med disse fordeler, innehar mesenchymale stamceller (MSC) funksjoner, slik som immunolodulatory effekt, gjør hDPSCs mer verdifull, selv i tilfelle av allograft transplantasjon<sup&gt; 6,9,10</sup&gt;. Derfor, er det primære trinnet for bruk av denne kilde til stamceller for å velge den beste protokollen for å isolere hDPSCs fra pulpavevet. For å oppnå dette målet, er det avgjørende å undersøke effekten av ulike isolasjon forhold på ulike cellulære atferd, for eksempel deres felles overflate markører og også deres differensiering kapasitet.</p&gt;<p class="jove_content"&gt; Derfor, her vi skiller mennesker pulpavevet fra påvirket tredje molar tenner, og deretter brukt både eksisterende protokoller basert på litteratur, for å isolere hDPSCs,<sup&gt; 11-13</sup&gt;<em&gt; Dvs.</em&gt; Enzymatisk dissosiasjon av pulpavevet (DPSC-ED) eller utvekst fra vev explants (DPSC-OG). I denne forbindelse, prøvde vi å legge til rette for isolasjon metoder ved hjelp av dental diamant disk. Deretter disse cellene preget i form av stromal-tilknyttede Markers (CD73, CD90, CD105 og CD44), blodkreft / endothelial markører (CD34, CD45 og CD11b), perivaskulær markør, som CD146 og også STRO-1. Etterpå ble disse to protokollene sammenlignet basert på differensiering styrke i odontoblaster både kvantitative polymerase chain reaction (QPCR) og Alizarin Red Farging. QPCR ble brukt for vurdering av uttrykket av mineralisering-relaterte gener (alkalisk fosfatase, ALP, matrix ekstracellulært phosphoglycoprotein, MEPE &amp; dentin sialophosphoprotein, DSPP).<sup&gt; 14</sup</p

<ol><li> DPSCs som ble innhentet ved enzymatisk dissosiasjon (DPSC-ED) er vist her på 10 dag, 15,18 (figur 1). Koloniene iverksatt for å danne på nesten 3 til 5 dager etter isolasjon. </li><li> Outgrown DPSCs (DPSC-OG) er vist i figur 2 på 5 dag, 10, 13 &amp; 18. Fibroblast-lignende celler begynte å migrere fra pulpavevet i flaska ved parallelle bestilling av nesten 5 dager etter såing. </li><li> DPSCs på passasjen 3 med begge metoder er vist i figur 3.. Begge typer DPSCs var nesten i sa...

Watch this Scientific Journal Video about Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods at JoV

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Metoden er beskrevet isolering og karakterisering av menneskelig tannpulpa stamceller (hDPSCs) ved hjelp av enten<strong&gt; Enzymatisk dissosiasjon av papirmasse (DPSC-ED)</strong&gt; Eller<strong&gt; Direkte resultat av stamceller fra pulpavevet explants (DPSC-OG). Deretter fulgt av</strong&gt;<em&gt; In vitro</em&gt; Sammenlignende differensiering av begge typer hDPSCs inn odontoblaster.

Isolasjon, karakterisering og Comparative Differensiering av Human tannpulpa stamceller hentet fra permanente tenner ved hjelp av to ulike metoder

Developing wisdom teeth are easy-accessible source of stem cells during the adulthood which could be obtained by routine orthodontic treatments. Human ...

Research

JoVE Journal

Medicine

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Pasient avledet celle Kultur og Isolering av CD133<sup&gt; +</sup&gt; Antatte kreft stamceller fra melanom

Despite improved treatments options for melanoma available today, patients with advanced malignant melanoma still have a poor prognosis for ...

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

Isolasjon og Funksjonell karakterisering av menneskelig Ventrikulær Cardiomyocytes fra Fresh Kirurgiske Samples

Cardiomyocytes from diseased hearts are subjected to complex remodeling processes involving changes in cell structure, excitation contraction coupling and ...

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Isolering av kreft stamceller fra menneskelige prostata kreft Samples

The cancer stem cell (CSC) model has been considerably revisited over the last two decades. During this time CSCs have been identified and directly ...

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens

To metoder for å etablere primær Menneskelig Livmor stromale celler fra Hysterektomi Prøver

Many efforts have been devoted to establish in vitro cell culture systems. These systems are designed to model a vast number of in vivo processes. Cell ...

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

Berikelse for kjemoresistent eggstokkreft stamceller fra humane cellelinjer

Cancer stem cells (CSCs) are defined as a subset of slow cycling and undifferentiated cells that divide asymmetrically to generate highly proliferative, ...

Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

Isolasjon og Cellular fenotyping av stamceller Avledet fra leddvæske og benmarg av minigriser

Mesenchymal stem cells (MSCs) have been established after isolation from various tissue sources, including bone marrow and synovial fluid. Recently, ...

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Karakteristikk av immunceller i menneskelig fettvev ved hjelp av flowcytometri

Infiltration of immune cells in the subcutaneous and visceral adipose tissue (AT) deposits leads to a low-grade inflammation contributing to the ...

Oral Biofilm Formation on Different Materials for Dental Implants

Muntlig Biofilm formasjon på ulike materialer for tannimplantater

Dental implants and their prosthetic components are prone to bacterial colonization and biofilm formation. The use of materials that provides low ...

An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

Et enzym-fri metode for isolering og utvidelse av Human fett-avledet Mesenchymal stamceller

Mesenchymal stem cells (MSCs) are a population of multipotent cells that can be isolated from various adult and fetal tissues, including adipose tissue. ...

Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

Ex Vivo utvidelse av blodkreft stamceller fra menneskelige navlestreng blod-avledet CD34+ celler ved hjelp av Valproic Acid

Umbilical cord blood (UCB) units provide an alternative source of human hematopoietic stem cells (HSCs) for patients who require allogeneic bone marrow ...