Name: integrated photoacoustic ophthalmoscopy and spectral-domain optical coherence tomography
Uploaded: 2013-01-15T12:00:00
Description: Watch this Scientific Journal Video about Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography at JoVE.com

我们感谢美国国家科学基金会（职业CBET-1055379）和美国国立卫生研究院（1RC4EY021357，1R01EY019951）的鼎力支持。我们还认识到支持从宋炜中国国家留学基金管理委员会。

1。系统配置<ol><li> PAOM子系统 <ol><li>照明光源：Nd：YAG激光（SPOT-10-100，UK：Elforlight有限公司，20μJ/脉冲; 2纳秒脉冲持续时间; 30 kHz的最大脉冲重复频率）。 </li><li>输出激光波长为1064 nm倍频532 nm的β- - 硼酸钡（BBO）晶体（福建福晶科技，圣何塞，CA）。进一步分割通过激光线镜像后，交付532 nm的光通过单模光纤（P1-460A-FC-5，Thorlabs公司），和1064 nm激光记录由一个光电二极管（DET10A，Thorlabs?...

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	<li>Kinyoun, J. L., Martin, D. C., Fujimoto, W. Y., Leonetti, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ophthalmoscopy+versus+fundus+photographs+for+detecting+and+grading+diabetic+retinopathy.">Ophthalmoscopy versus fundus photographs for detecting and grading diabetic retinopathy.</a> Invest. Ophthalmol. Vis. Sci. 33 (6), 1888-1893 (1992).</li><li>Schuman, J. S., Wollstein, G., Farra, T., Hertzmark, E., Aydin, A., Fujimoto, J. G., Paunescu, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+optic+nerve+head+measurements+obtained+by+optical+coherence+tomography+and+confocal+scanning+laser+ophthalmoscopy.">Comparison of optic nerve head measurements obtained by optical coherence tomography and confocal scanning laser ophthalmoscopy.</a> Am. J. 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V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiscale+photoacoustic+microscopy+and+computed+tomography.">Multiscale photoacoustic microscopy and computed tomography.</a> Nat. Photonics. 3 (9), 503-509 (2009).</li><li>Jiao, S., Jiang, M., Hu, J., Fawzi, A., Zhou, Q., Shung, K. K., Puliafito, C. A., Zhang, H. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photoacoustic+ophthalmoscopy+for+in+vivo+retinal+imaging.">Photoacoustic ophthalmoscopy for in vivo retinal imaging.</a> Opt. Express. 18 (4), 3967-3972 (2010).</li><li>Wei, Q., Liu, T., Jiao, S., Zhang, H. 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在这里，我们提出了一个详细的说明同时在体内视网膜大鼠的用眼PAOM结合SD-OCT成像。 ，或许，基于光散射SD-OCT视网膜成像3临床“金标准”，但是，这是不敏感的检测在视网膜上的光吸收。新开发的PAOM是基于光学吸收的唯一的眼科成像方式，提供的光吸收特性的视网膜6。 ，因为血红蛋白和黑色素是内生性较强的光吸收色素，PAOM使调查的解剖和功能的视网膜RPE /脉络膜血管...

integrated photoacoustic ophthalmoscopy and spectral-domain optical coherence tomography

极大地受益于各种非侵入性的眼科成像技术的重大眼部疾病的临床诊断和基本的调查。现有的视网膜成像方式，如共焦激光扫描眼底镜检查（CSLO）2，眼底照相，光学相干断层扫描（OCT），有显着贡献，监测疾病的起始和级数，并开发新的治疗策略。然而，它们主要依靠的背面反射的光子从视网膜。因此，在视网膜的光吸收特性，这通常是紧密联系在一起的视网膜病理生理状态，是无法由传统的成像技术。 “光声眼底镜检查（PAOM）是一种新兴的的视网膜成像方式，允许在眼内的光吸收对比检测，具有灵敏度高4-7。在PAOM南交付osecond激光脉冲通过瞳孔扫过的眼后诱导光声（PA）检测到的信号，这是由一个未聚焦的超声波换能器连接到眼睑。因为强烈的光吸收的血红蛋白和黑色素，PAOM是能够非侵入性成像视网膜和脉络膜vasculatures的，而视网膜色素上皮细胞（RPE）在高对比度的黑色素6,7。更重要的是，发达的光声光谱成像5,8的基础上，PAOM有可能映射在视网膜新生血管中的血红蛋白氧饱和度，它可以致盲性疾病，如糖 ​​尿病性视网膜病变的生理和病理研究的关键和新生血管性年龄相关性黄斑变性。 此外，现有的光学吸收为基础的眼科影像学检查方法，PAOM可以集成完善的临床眼科成像TEchniques实现多个光学对比6,10基础上的眼睛，更全面的解剖和功能的评价。在这项工作中，我们整合PAOM和谱域OCT（SD-OCT），用于同时在体内视网膜成像大鼠，其中揭示的视网膜的光的吸收和散射特性。的系统配置，系统校准和图像采集。

图2示出的2-D SD-OCT和PAOM白化病大鼠（A和B）和着色大鼠（C和D），分别在同时获得的眼底图像。在SD-OCT的眼底图像（ 图2A和2C），视网膜血管有深色的外观，由于探测光的血红蛋白吸收。 PAOM除了视网膜血管（ 图2B中的RV），可视化的的脉络膜vasculatures（CV在图2B）在白化病眼，因为缺乏RPE黑色素。由于色素的眼睛具有高黑?...

Watch this Scientific Journal Video about Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography at JoVE.com

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

光声眼科（PAOM）的，一个基于光学吸收的成像模态，提供了互补的评价的视网膜的当前可用的眼科成像技术。我们报告的使用PAOM集成与谱域光学相干断层扫描（SD-OCT），同时多式联运大鼠视网膜成像。

集成光声眼底镜检查和谱域光学相干断层扫描

Both the clinical diagnosis and fundamental investigation of major ocular diseases greatly benefit from various non-invasive ophthalmic imaging ...

Research

JoVE Journal

Bioengineering

Three-dimensional Optical-resolution Photoacoustic Microscopy

三维光学分辨率的光声显微镜

Optical microscopy, providing valuable insights at the cellular and organelle levels, has been widely recognized as an enabling biomedical technology. As ...

科研

生物工程

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

使用光声血流循环黑色素瘤细胞的检测和隔离

Circulating tumor cells (CTCs) are those cells that have separated from a macroscopic tumor and spread through the blood and lymph systems to seed ...

Photoacoustic Cystography

光声膀胱造影

Conventional pediatric cystography, which is based on diagnostic X-ray using a radio-opaque dye, suffers from the use of harmful ionizing radiation. The ...

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

含有金纳米棒蜂窝车辆的制备及光声分析

Gold nanorods are attractive for a range of biomedical applications, such as the photothermal ablation and the photoacoustic imaging of cancer, thanks to ...

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

计算机断层扫描和成骨血管生成耦合光学成像评估头盖骨自体移植和移植物的整合

A major parameter determining the success of a bone-grafting procedure is vascularization of the area surrounding the graft. We hypothesized that ...

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

可切换声光学分辨率光声显微镜<em&gt;体内</em&gt;小动物血液血管成像

Photoacoustic microscopy (PAM) is a fast-growing invivo imaging modality that combines both optics and ultrasound, providing penetration beyond the ...

A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

高性能紧凑型光声层析成像系统<em&gt;体内</em&gt;小动物脑成像

In vivo small-animal imaging has an important role to play in preclinical studies. Photoacoustic tomography (PAT) is an emerging hybrid imaging modality ...

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

斜扫描激光检眼镜 (奥斯陆) 与光学相干层析成像 (OCT) 的多模态视网膜成像

While fluorescence imaging is widely used in ophthalmology, a large field of view (FOV) three-dimensional (3D) fluorescence retinal image is still a big ...

Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

基于光学相干断层扫描的可降解支架组织工程血管发育的无损监测

Engineered vascular grafts with structural and mechanical properties similar to natural blood vessels are expected to meet the growing demand for arterial ...

Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature

基于脉冲激光二极管的桌面光声层析成像在大鼠皮质血管内监测染料的洗入和洗脱

Photoacoustic (PA) tomography (PAT) imaging is an emerging biomedical imaging modality useful in various preclinical and clinical applications. ...