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DOI :
10.3791/4393-v
February 13th, 2013
Chapters
0:05
Title
2:43
Treatment of Cells with Thrombin, RNA Isolation, Quality Assessment and Quantification of RNA
3:54
Library Construction and Sequencing
8:22
Data Analysis
12:16
Results: RNA-seq Analysis Output
17:16
Conclusion
このプロトコルは、トロンビン処理の有無にかかわらず、ヒト肺微小血管内皮細胞にトランスクリプトームのプロファイルへのRNA-seqの、強力な次世代DNAシーケンシング技術を適用するための完全かつ詳細な手順を示します。このプロトコルは、異なる試薬又は病気の状態によって影響を受ける様々な細胞や組織に一般化できる。
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