Name: probe-based confocal laser endomicroscopy of the urinary tract: the technique
Uploaded: 2013-01-10T12:00:00
Description: Watch this Scientific Journal Video about Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique at JoVE.com

作者要感谢莫纳克亚山科技的技术支持。作者还感谢萧雪莉的技术援助和Kathleen E.马赫的严格审查。这项工作是支持的，部分由美国国立卫生研究院R01CA160986 JCL

1。患者准备<ol><li>同意定为病人诊断膀胱镜检查和其他腔内的程序，如经尿道膀胱肿瘤切除术（TURBT）CLE。包括在同意膀胱内和/或静脉内的荧光素作为造影剂的使用的描述。询问的过敏性反应与荧光素的历史。 </li><li>病人被定位为膀胱镜检查（一般在截石位），并准备在无菌的方式。 </li><li>继续进行，标准白光膀胱镜检查（WLC）具有刚性或柔性的膀胱镜通过尿道。 </li><li>在白色灯光下（ <stron...

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获得和维持稳定的成像探头和膀胱粘膜的连接面之间的接触中获得最佳的图像质量是最关键的一步。有大约3-5个病人的学习曲线发展的灵巧操纵成像探测，并在图像采集过程中保持稳定的探测。此外，作为本程序是在体内进行的，患者的动作（ 即呼吸）和血管的脉动可能影响膀胱的成像探针接触。观察者之间的差异和学习曲线的图像解释，这需要一定的经验与广泛的膀胱病变...

<table border="1"><tbody><tr><td> 的试剂的名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td> Cellvizio 100系列</td><td>莫纳克亚山</td><td> 100系列</td><td>包括共聚焦的处理器和488 nm处的LSU：F400-V2 </td></tr><tr><td> Cellvizio共聚焦微探头</td><td>莫纳克亚山</td><td> Gastroflex UHD </td><td></td></tr><tr><td> AK-FLUOR 10％ </td><td> Akorn公司</td><td> NDC 17478-253-10 </td><td></td></tr></tbody></table>

probe-based confocal laser endomicroscopy of the urinary tract: the technique

基于探测器的激光共聚焦激光显微内镜（CLE）是一种新兴的光学成像技术，可以实时在体内显微镜在标准内镜粘膜表面。 2-6 CLE应用程序中的呼吸和胃肠道，还探讨了在尿道膀胱癌的诊断。7-10细胞形态和组织微体系架构，除了可以解决与微米级分辨率的实时动态影像的正常及病理血管。 基于探测器CLE系统（Cellvizio马纳凯亚技术，法国）包括一个可重复使用的光纤耦合到488 nm的激光扫描单元的成像探针。成像探头插入标准的柔性和刚性的内窥镜中的工作通道。的内窥镜系CLE系统（OPTISCAN，澳大利亚），在其中的共聚焦显微内镜functionality被集成到的内窥镜，也用作在胃肠道中。然而，给定的范围更大的直径，在泌尿道的应用目前只限于体外使用。11共聚焦图像采集是通过与靶组织的成像探针的直接接触，并记录为视频序列。在胃肠道中，显微内镜的尿路需要一个exogenenous的的造影剂的最常用的荧光素，可以静脉内给药或膀胱内注射。膀胱管理是一个行之有效的方法，以最小的全身毒性是唯一的尿路介绍药物的局部。荧光快速污渍的细胞外基质，并已建立了的安全性。各种直径的12个成像探针与不同口径的内窥镜启用兼容性。至目前为止，1.4毫米和2.6毫米探针进行了评估，灵活和里GID膀胱镜检查。最近推出的&lt;1 mm的成像探头13骑士在输尿管镜在上尿路的可能性。荧光膀胱镜检查（ 即光动力诊断）和窄带成像的附加 ​​型内窥镜为基础的光学成像方式14，可以结合CLE实现多模态成像的尿路。在未来，CLE可以与分子造影剂，如荧光标记的肽15和抗体与分子的特异性疾病的进程用于内窥镜成像。

CLE图像保存为灰度每秒12帧的视频序列文件。图片的解释是实时完成的，并可能影响到临床决策的研究协议下。离线分析，其中包括检讨的视频序列中，附加的图像处理，如拼接，7和比较，与标准的病理学中，是重要的期间与该技术相关的学习曲线相。图1B和1C是代表CLE从两个不同的膀胱肿瘤膀胱内和静脉内的荧光素，分别获得的图像。对于膀胱肿瘤细胞的功能（ 如形态，凝聚力?...

Watch this Scientific Journal Video about Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique at JoVE.com

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

基于探测器的激光共聚焦激光显微内镜可实时显微镜的人尿路，膀胱镜检查过程中，提供动态的，活体病理状态，如膀胱癌的细胞高分辨率成像。显微内镜可以增加诊断的准确率的标准白光内窥镜检查和术中图像提供指导，以提高手术切除。

基于探测器的激光共聚焦激光显微内镜对泌尿道的技术

Probe-based  confocal laser endomicroscopy (CLE) is an emerging optical imaging technology  that enables real-time in vivo microscopy of mucosal surfaces ...

Research

JoVE Journal

Medicine

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

腭扩展口腔生物膜分析，荧光原位杂交和激光共聚焦显微镜

Confocal laser scanning microscopy (CLSM) of natural heterogeneous biofilm is today facilitated by a comprehensive range of staining techniques, one of ...

科研

医学

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

膀胱平滑肌收缩带作为一种方法来评估下尿路药理学

We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and pharmacological properties ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

玫瑰红Photothrombosis共聚焦光学成像<em&gt;在体内</em&gt;：单支脑卒中模型

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

使用层压技术来执行人类巩膜共聚焦显微镜


The sclera is a dense connective tissue that covers and protects the eye. It mainly consists of dense collagen bundles (types I, III, IV, V, VI, and ...

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS

在脑组织成像金属激光剥蚀 - 电感耦合等离子体 - 质谱法（LA-ICP-MS）

Metals are found ubiquitously throughout an organism, with their biological role dictated by both their chemical reactivity and abundance within a ...

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry LCM-LC/MS

激光捕获显微切割液相色谱质谱 (LCM-LC/MS) 对肺结核患者药物的空间量化

Tuberculosis is still a leading cause of morbidity and mortality worldwide. Improvements to existing drug regimens and the development of novel ...

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

用于尿功能测试的成人男性尿道超声检查

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting ...

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

上尿道泌尿管在上尿道泌尿管切除术期间的技术改造

Upper tract urothelial carcinoma (UTUC) accounts for 5%&ndash;10% of all urothelial tumors. Radical nephroureterectomy is the standard treatment ...

Laser Doppler Perfusion Imaging in the Mouse Hindlimb

激光多普勒灌注成像在鼠标欣德利姆

Blood flow recovery is a critical outcome measure after experimental hindlimb ischemia or ischemia-reperfusion. Laser Doppler perfusion imaging (LDPI) is ...

Characterization of a Novel Human Organotypic Retinal Culture Technique

新型人类组织特征视网膜培养技术的特征

Previous human organotypic retinal culture (HORC) models have utilized detached retinas; however, without the structural support conferred by retinal ...