Name: probe-based confocal laser endomicroscopy of the urinary tract: the technique
Uploaded: 2013-01-10T12:00:00
Description: Watch this Scientific Journal Video about Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique at JoVE.com

Forfatterne vil gerne takke Mauna Kea Technologies for teknisk support. Forfatterne takker også Shelly Hsiao for teknisk assistance og Kathleen E. Mach til kritisk gennemgang. Dette arbejde blev støttet delvist af NIH R01CA160986 til JCL

1. Patientforberedelse <ol><li> Samtykke patient er planlagt til diagnostisk cystoskopi og andre endourological procedurer, såsom transuretral resektion af blære tumor (TURBT) for CLE. Omfatter i tilladelsen en beskrivelse af anvendelsen af ​​intravesical og / eller intravenøs fluorescein som kontrastmidlet. Spørge historie overfølsomhedsreaktion til fluorescein. </li><li> Patienten anbringes for cystoskopi (typisk i lithotomy position) og fremstilles på steril måde. </li><li> Fortsæt med standard hvidt ly...

<ol>
	<li>Thiberville, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+in+vivo+fluorescence+microimaging+of+the+alveolar+ducts+and+sacs+during+bronchoscopy.">Human in vivo fluorescence microimaging of the alveolar ducts and sacs during bronchoscopy.</a> Eur. Respir. J. 33, 974-985 (2009).</li><li>Dunbar, K. B., Okolo, P., Montgomery, E., Canto, M. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Confocal+laser+endomicroscopy+in+Barrett's+esophagus+and+endoscopically+inapparent+Barrett's+neoplasia:+a+prospective,+randomized,+double-blind,+controlled,+crossover+trial.">Confocal laser endomicroscopy in Barrett's esophagus and endoscopically inapparent Barrett's neoplasia: a prospective, randomized, double-blind, controlled, crossover trial.</a> Gastrointest. Endosc. 70, 645-654 (2009).</li><li>Buchner, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+probe-based+confocal+laser+endomicroscopy+with+virtual+chromoendoscopy+for+classification+of+colon+polyps.">Comparison of probe-based confocal laser endomicroscopy with virtual chromoendoscopy for classification of colon polyps.</a> Gastroenterology. 138, 834-842 (2010).</li><li>Pech, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Confocal+laser+endomicroscopy+for+in+vivo+diagnosis+of+early+squamous+cell+carcinoma+in+the+esophagus.">Confocal laser endomicroscopy for in vivo diagnosis of early squamous cell carcinoma in the esophagus.</a> Clin. Gastroenterol. Hepatol. 6, 89-94 (2008).</li><li>Goetz, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+confocal+laser+endo+microscopy+of+the+human+liver:+a+novel+method+for+assessing+liver+microarchitecture+in+real+time.">In vivo confocal laser endo microscopy of the human liver: a novel method for assessing liver microarchitecture in real time.</a> Endoscopy. 40, 554-562 (2008).</li><li>Meining, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+cholangiocarcinoma+in+vivo+using+miniprobe-based+confocal+fluorescence+microscopy.">Detection of cholangiocarcinoma in vivo using miniprobe-based confocal fluorescence microscopy.</a> Clin. Gastroenterol. Hepatol. 6, 1057-1060 (2008).</li><li>Wu, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+real-time+microscopy+of+the+urinary+tract+using+confocal+laser+endomicroscopy.">Dynamic real-time microscopy of the urinary tract using confocal laser endomicroscopy.</a> Urology. 78, 225-231 (2011).</li><li>Sonn, G. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+biopsy+of+human+bladder+neoplasia+with+in+vivo+confocal+laser+endomicroscopy.">Optical biopsy of human bladder neoplasia with in vivo confocal laser endomicroscopy.</a> J. Urol. 182, 1299-1305 (2009).</li><li>Sonn, G. 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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=First+assessment+of+needle-based+confocal+laser+endomicroscopy+during+EUS-FNA+procedures+of+the+pancreas+(with+videos.">First assessment of needle-based confocal laser endomicroscopy during EUS-FNA procedures of the pancreas (with videos.</a> Gastrointest. Endosc. 74, 1049-1060 (2011).</li><li>Liu, J. -. J., Droller, M., Liao, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+Optical+Imaging+Technologies+in+Bladder+Cancer:+Considerations+and+Perspectives.">New Optical Imaging Technologies in Bladder Cancer: Considerations and Perspectives.</a> J. Urol. 188, 361-368 (2012).</li><li>Hsiung, P. -. 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Opnåelse og fastholdelse af solid en face kontakt mellem den billeddannende sonden og blære slimhinden er den mest kritiske skridt i at erhverve optimal billedkvalitet. Der er cirka en 3-5 patient indlæringskurve at udvikle fingerfærdighed til at manipulere den billeddannende sonden og at holde sonden konstant under billedoptagelse. Desuden, når denne fremgangsmåde udføres in vivo, kan patientens bevægelser (dvs. luftvejs) og vaskulære pulsationer påvirker imaging probe kontakt med b...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer (valgfrit) </td></tr><tr><td> Cellvizio 100 Series </td><td> Mauna Kea Technologies </td><td> 100-serien </td><td> Inkluderer konfokal processor og LSU: F400-v2 ved 488 nm </td></tr><tr><td> Cellvizio Konfokal Miniprobe </td><td> Mauna Kea Technologies </td><td> Gastroflex UHD </td><td></td></tr><tr><td> AK-FLUOR 10% </td><td> Akorn, Inc. </td><td> NDC 17478-253-10 </td><td></td></tr></tbody></table>

probe-based confocal laser endomicroscopy of the urinary tract: the technique

Probe-baseret konfokalt laser endomicroscopy (CLE) er en ny optisk imaging-teknologi, der muliggør real-time in vivo mikroskopi af slimhindeoverflader under standard endoskopi. Med applikationer, der aktuelt i luftvejene 1 og gastrointestinale skrifter, har 2-6 CLE også blevet undersøgt i urinvejene for blærekræft diagnose. 7-10 Cellular morfologi og væv mikroarkitektur kan løses med micron skala opløsning i realtid, i tillæg til dynamisk billeddannelse af normale og patologiske vaskulatur. 7 Probe-baserede CLE system (Cellvizio, Mauna Kea Technologies, France) består af en genanvendelig fiberoptisk billeddannende probe koblet til en 488 nm laser scanning enhed. Den billeddannende sonde anbringes i arbejdsgrupperne kanaler standard fleksible og stive endoskoper. Et endoskop-baseret CLE system (Optiscan, Australien), hvor den konfokale endomicroscopy functionality er integreret på endoskopet, anvendes også i mave-tarmkanalen. I betragtning af den større anvendelsesområde diameter, imidlertid er anvendelsen i urinvejene i øjeblikket begrænset til ex vivo-anvendelse. 11 Konfokal billedoptagelse sker gennem direkte kontakt af den billeddannende probe med mål-væv og registreres som videosekvenser. Som i mavetarmkanalen, kræver endomicroscopy af urinvejene et exogenenous kontrastmiddel-hyppigst fluorescein, som kan administreres intravenøst ​​eller intravesikalt. Intravesikal administration er en veletableret fremgangsmåde til at indføre farmakologiske midler lokalt med minimal systemisk toksicitet, der er unik for urinvejene. Fluorescein hurtigt pletter den ekstracellulære matrix og har en etableret sikkerhedsprofil. 12 Imaging sonder med forskellige diametre muliggøre kompatibilitet med forskellige kaliber endoskoper. Til dato har 1,4 og 2,6 mm prober blevet evalueret med fleksibel og riGID cystoskopi. 10 Recent adgang til en &lt;1 mm imaging probe 13 åbner mulighed for CLE i de øvre urinveje under ureteroscopy. Fluorescens cystoskopi (dvs. fotodynamisk diagnose) og smalle bånd billeddannelse er yderligere endoskop-baserede optiske afbildningsmodaliteter 14, der kan kombineres med CLE at opnå multimodal billeddannelse af urinvejene. I fremtiden kan CLE kobles med molekylære kontrastmidler, såsom fluorescensmærkede peptider 15 og antistoffer til endoskopisk billeddannelse af sygdomsprocesser med molekylær specificitet.

CLE billeder gemmes som gråtoner videosekvensdele filer ved 12 frames per sekund. Billede fortolkning sker i realtid og kan anvendes til at påvirke den kliniske beslutningsproces under en testpræparater protokol. Offline analyse, som omfatter revision af videosekvensen, ekstra billedbehandling, såsom mosaicing, 7 og sammenligning med standard patologi, er vigtige i den indlæringskurven fase er forbundet med teknologien. 1B og 1C er repræsentative CLE billeder opnået fra to forskellige blæretumorer ved...

Watch this Scientific Journal Video about Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique at JoVE.com

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

Sondebaseret konfokal laser endomicroscopy muliggør real-time mikroskopi af den humane urinvejene under cystoskopi, tilvejebringe dynamisk, intravital billeddannelse af patologiske tilstande, såsom blærecancer med cellulær opløsning. Endomicroscopy kan forstærke den diagnostiske nøjagtighed standard hvidt lys endoskopi og give intraoperativ billede vejledning til at forbedre kirurgisk resektion.

Probe-baseret Konfokal Laser Endomicroscopy af urinvejene: Teknikken

Probe-based  confocal laser endomicroscopy (CLE) is an emerging optical imaging technology  that enables real-time in vivo microscopy of mucosal surfaces ...

Research

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Medicine

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We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and pharmacological properties ...

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In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

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Ved hjælp af en Laminering teknik til at udføre Konfokal mikroskopi af det menneskelige Sclera


The sclera is a dense connective tissue that covers and protects the eye. It mainly consists of dense collagen bundles (types I, III, IV, V, VI, and ...

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Metals are found ubiquitously throughout an organism, with their biological role dictated by both their chemical reactivity and abundance within a ...

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Rumlige kvantificering af narkotika i lungetuberkulose læsioner af Laser fange Microdissection Liquid Chromatography massespektrometri (LCM-LC/MS)

Tuberculosis is still a leading cause of morbidity and mortality worldwide. Improvements to existing drug regimens and the development of novel ...

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The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting ...

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Teknisk modifikation af Terminal ureter under Total Transperitoneal laparoskopisk Nephroureterectomy for øvre urinvejs Urothelial karcinom

Upper tract urothelial carcinoma (UTUC) accounts for 5%&ndash;10% of all urothelial tumors. Radical nephroureterectomy is the standard treatment ...

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Laser Doppler Perfusion Imaging i musen hindlimb

Blood flow recovery is a critical outcome measure after experimental hindlimb ischemia or ischemia-reperfusion. Laser Doppler perfusion imaging (LDPI) is ...

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Karakterisering af en ny human organotypic retinal kultur teknik

Previous human organotypic retinal culture (HORC) models have utilized detached retinas; however, without the structural support conferred by retinal ...