Name: murine fetal echocardiography
Uploaded: 2013-02-15T12:00:00
Description: Watch this Scientific Journal Video about Murine Fetal Echocardiography at JoVE.com

GHK由NIH / NHLBI K08-HL098565和研究所在芝加哥大学心血管研究的支持。在芝加哥大学实验动物护理和使用委员会批准的所有描述的实验方法。

1。准备成像的小鼠<ol><li>在此之前的影像学研究，麻醉诱导室大坝（2-3％异氟醚）。将动物的感应室，并立即将口鼻部连接的麻醉系统内的鼻锥。电推剪，删除皮草从中期胸部下肢（参见图1）。取出剩余的体毛，脱毛霜。脱毛霜也可用于无电推剪，应彻底洗掉后皮肤使用，以防止刺激。 </li><li>将麻醉小鼠在仰卧位嵌入式心电图上的一个加热垫，导致以保持身体的温度（ 图1?...

<ol>
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进行串行测量和检测突变的胎儿心脏缺陷的能力，突出的实用性超声心动图研究正常和不正常的心血管疾病的发展。 体内的心脏结构和功能的分析已成为胎儿的正常发育遗传和非遗传修饰的描述中不可或缺的一部分。 2D制导多普勒的可用性使得它可以监测心脏率和血流模式的同时，获得实时图像。发展可能存在心脏畸形，如室间隔缺损和检测。尽管当前的成像平台的高分辨率能力，收购高...

<table border="1"><tbody><tr><td> VEVO 770成像系统 </td><td> VisualSonics </td><td></td><td> （加拿大，多伦多） </td></tr><tr><td> RMV707B.15-45 MHz传感器</td><td></td><td></td><td></td></tr><tr><td> Tec的异氟醚蒸发器</td><td></td><td></td><td></td></tr><tr><td>异氟醚（2 - 氯-2 - （二氟甲氧基）-1,1,1 - 三氟乙烷） </td><td></td><td></td><td></td></tr></tbody></table>

murine fetal echocardiography

转基因小鼠显示心脏的发育和功能的异常，正常心血管功能和人体心血管疾病的病理生理基础的分子机制的理解一个强大的工具。胎儿和围产期死亡是一个共同的特点，在研究基因的改变影响心脏发育1-3。为了研究遗传或药物的变化，在发展的初期心功能的作用，早期识别异常和纵向后续的活胎儿的超声成像已经成为一个重要的工具。无创性的超声显像是一种理想的方法，用于检测和研究先天畸形和心功能的影响之前，死亡4。它允许在客厅胎儿的异常和疾病的进展可以跟在子宫内的纵向研究5,6的早期识别。直到最近，成像胎鼠心脏经常涉及侵入性的方法。胎儿必须进行磁共振显微镜和电子显微镜或透射镜手术交付的牺牲。高频探头与传统2-D和脉冲多普勒成像中的应用已被证明在胚胎发育过程中提供测量心脏收缩和心脏率与数据库的正常发育变化，现已6-10。 M模式成像进一步提供重要的功能的数据，虽然，往往很难获得适当的成像平面。高频超声成像对胎儿已提高了2-D分辨率，心脏结构11的早期发展，并能提供优秀的信息。

Watch this Scientific Journal Video about Murine Fetal Echocardiography at JoVE.com

Murine Fetal Echocardiography

学习时，胎儿和围产期死亡的共同特点是影响心脏发育的基因的改变。高频超声成像已经提高了2-D分辨率，可以提供优秀的信息对早期心脏发育是一个理想的方法来检测心脏结构和功能的影响，在死亡前。

小鼠胎儿超声心动图

Transgenic mice displaying abnormalities in  cardiac development and function represent a powerful tool for the understanding  the molecular mechanisms ...

Research

JoVE Journal

Medicine

Transthoracic Echocardiography in Mice

经胸超声心动图在小鼠

In recent years, murine models have become the primary avenue for studying the molecular mechanisms of cardiac dysfunction resulting from changes in gene ...

科研

医学

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

高频超声心动图：非侵入性的反复测量小鼠心脏缺血再灌注心肌劳损，收缩力，二尖瓣关闭不全的第一个证据

Ischemia-reperfusion (IR) was surgically performed in murine hearts which were then subjected to repeated imaging to monitor temporal changes in ...

Murine Echocardiography and Ultrasound Imaging

小鼠超声心动图和超声成像

Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics.1  ...

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

在斑马鱼模型的胎儿酒精暴露评估致畸的变化

Fetal alcohol syndrome (FAS) is a severe manifestation of embryonic exposure to ethanol. It presents with characteristic defects to the face and organs, ...

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction

在家兔模型宫内生长受限胎儿超声心动图和多普勒超声脉冲波

Fetal intrauterine growth restriction (IUGR) results in abnormal cardiac function that is apparent antenatally due to advances in fetoplacental Doppler ...

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

分离，培养和成像胎儿胰腺细胞集群

For almost 30 years, scientists have demonstrated that human fetal ICCs transplanted under the kidney capsule of nude mice matured into functioning ...

A Mouse Fetal Skin Model of Scarless Wound Repair

对无瘢痕修复鼠标胎儿皮肤模型

Early in utero, but not in postnatal life, cutaneous wounds undergo regeneration and heal without formation of a scar. Scarless fetal wound healing occurs ...

Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery

左心房，主动脉和肺动脉的超声心动图小鼠

The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic ...

2D and 3D Echocardiography in the Axolotl (Ambystoma Mexicanum)

2D and 3D Echocardiography in the Axolotl Ambystoma Mexicanum

axolotl 中的二维和三维超声心动图

Cardiac malfunction as a result of ischemic heart disease is a major challenge, and regenerative therapies to the heart are in high demand. A few model ...

Model Surgical Training: Skills Acquisition in Fetoscopic Laser Photocoagulation of Monochorionic Diamniotic Twin Placenta Using Realistic Simulators

模型外科训练: 用逼真模拟器 Fetoscopic 激光光凝单绒毛膜 Diamniotic 双胎胎盘的技能习得

Fetoscopic laser coagulation of arterio-venous anastomoses (AVA) in a monochorionic placenta is the standard of care for twin-twin transfusion syndrome ...