Name: murine fetal echocardiography
Uploaded: 2013-02-15T12:00:00
Description: Watch this Scientific Journal Video about Murine Fetal Echocardiography at JoVE.com

GHK er støttet af NIH / NHLBI K08-HL098565 og Institut for Cardiovascular Research ved University of Chicago. Alle eksperimentelle beskrevne metoder er godkendt af Institutional Animal Care og brug Udvalg på University of Chicago.

1. Forberedelse Mus for Imaging <ol><li> Forud for den billeddannende undersøgelse bedøve dæmningen (2-3% isofluran) i induktionskammer. Fjern dyret fra induktionskammer og anbringes straks snuden i en næsekegle forbundet med anæstesi systemet. Fjern skind fra midten brysthøjde til underekstremiteterne (Se figur 1) med hårklippere. Fjern den resterende kropsbehåring med hårfjerningsmiddel. Hårfjerningsmiddel kan også anvendes uden hårklippere og skal omhyggeligt skylles af huden efter brug...

<ol>
	<li>Wessels, A., Sedmera, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+anatomy+of+the+heart:+a+tale+of+mice+and+man.">Developmental anatomy of the heart: a tale of mice and man.</a> Physiol. Genomics. 15, 165 (2003).</li><li>Snider, P., Conway, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Probing+human+cardiovascular+congenital+disease+using+transgenic+mouse+models.">Probing human cardiovascular congenital disease using transgenic mouse models.</a> Prog. Mol. Biol. Transl. Sci. 100, 83 (2011).</li><li>Clark, K. L., Yutzey, K. E., Benson, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcription+factors+and+congenital+heart+defects.">Transcription factors and congenital heart defects.</a> Annu. Rev. Physiol. 68, 97 (2006).</li><li>Leatherbury, L., Yu, Q., Lo, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+phenotypic+analysis+of+cardiovascular+structure+and+function+in+fetal+mice+using+ultrasound.">Noninvasive phenotypic analysis of cardiovascular structure and function in fetal mice using ultrasound.</a> Birth Defects Res C Embryo Today. 69, 83 (2003).</li><li>Spurney, C. F., Lo, C. W., Leatherbury, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fetal+mouse+imaging+using+echocardiography:+a+review+of+current+technology.">Fetal mouse imaging using echocardiography: a review of current technology.</a> Echocardiography. 23, 891 (2006).</li><li>Spurney, C. F., Leatherbury, L., Lo, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-frequency+ultrasound+database+profiling+growth,+development,+and+cardiovascular+function+in+C57BL/6J+mouse+fetuses.">High-frequency ultrasound database profiling growth, development, and cardiovascular function in C57BL/6J mouse fetuses.</a> J. Am. Soc. Echocardiogr. 17, 893 (2004).</li><li>Shen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiovascular+phenotyping+of+fetal+mice+by+noninvasive+high-frequency+ultrasound+facilitates+recovery+of+ENU-induced+mutations+causing+congenital+cardiac+and+extracardiac+defects.">Cardiovascular phenotyping of fetal mice by noninvasive high-frequency ultrasound facilitates recovery of ENU-induced mutations causing congenital cardiac and extracardiac defects.</a> Physiol. Genomics. 24, 23 (2005).</li><li>Yu, Q., Leatherbury, L., Tian, X., Lo, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiovascular+assessment+of+fetal+mice+by+in+utero+echocardiography.">Cardiovascular assessment of fetal mice by in utero echocardiography.</a> Ultrasound Med. Biol. 34, 741 (2008).</li><li>Linask, K. K., Huhta, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+Doppler+echocardiography+to+monitor+embryonic+mouse+heart+function.">Use of Doppler echocardiography to monitor embryonic mouse heart function.</a> Methods Mol. Biol. 135, 245 (2000).</li><li>Hinton, R. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+heart+valve+structure+and+function:+echocardiographic+and+morphometric+analyses+from+the+fetus+through+the+aged+adult.">Mouse heart valve structure and function: echocardiographic and morphometric analyses from the fetus through the aged adult.</a> Am. J. Physiol Heart Circ. Physiol. 294, H2480 (2008).</li><li>Gui, Y. H., Linask, K. K., Khowsathit, P., Huhta, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Doppler+echocardiography+of+normal+and+abnormal+embryonic+mouse+heart.">Doppler echocardiography of normal and abnormal embryonic mouse heart.</a> Pediatr. Res. 40, 633 (1996).</li><li>Purssell, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+high-resolution+ultrasound+reveals+structural+and+functional+deficits+in+dimethadione-exposed+fetal+rat+hearts+in+utero.">Noninvasive high-resolution ultrasound reveals structural and functional deficits in dimethadione-exposed fetal rat hearts in utero.</a> Birth Defects Res. B Dev. Reprod. Toxicol. , (2011).</li><li>Le, V. P., Kovacs, A., Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+Left+Ventricular+Pressure+in+Late+Embryonic+and+Neonatal+Mice.">Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice.</a> J. Vis. Exp. (60), e3756 (2012).</li><li>Ji, R. P., Phoon, C. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+localization+of+nuclear+factor+of+activated+T+cells+c1-/-+mouse+embryos+by+ultrasound+biomicroscopy-Doppler+allows+genotype-phenotype+correlation.">Noninvasive localization of nuclear factor of activated T cells c1-/- mouse embryos by ultrasound biomicroscopy-Doppler allows genotype-phenotype correlation.</a> J. Am. Soc. Echocardiogr. 18, 1415 (2005).</li><li>Kim, G. H., Samant, S. A., Earley, J. U., Svensson, E. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translational+control+of+FOG-2+expression+in+cardiomyocytes+by+microRNA-130a.">Translational control of FOG-2 expression in cardiomyocytes by microRNA-130a.</a> PLoS One. 4, e6161 (2009).</li><li>Momoi, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modest+maternal+caffeine+exposure+affects+developing+embryonic+cardiovascular+function+and+growth.">Modest maternal caffeine exposure affects developing embryonic cardiovascular function and growth.</a> Am. J. Physiol. Heart Circ. Physiol. 294, H2248 (2008).</li><li>Tobita, K., Liu, X., Lo, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+modalities+to+assess+structural+birth+defects+in+mutant+mouse+models.">Imaging modalities to assess structural birth defects in mutant mouse models.</a> Birth Defects Res. C Embryo Today. 90, 176 (2010).</li></ol>

Evnen til at udføre serielle målinger og til at detektere mutant fostre med kardiale defekter fremhæver nytten af ​​ekkokardiografi til undersøgelse normale og unormale kardiovaskulære udvikling. Analyse af hjertets struktur og funktion in vivo er blevet en integreret del af beskrivelsen af genetiske og ikke-genetiske modifikationer normal føtal udvikling. Tilgængeligheden af ​​2D-styrede Doppler gør det muligt at overvåge puls og blodgennemstrømning mønstre, mens du får real-time billeder. ...

<table border="1"><tbody><tr><td> Vevo 770 Imaging System </td><td> VisualSonics </td><td></td><td> (Toronto, Canada) </td></tr><tr><td> RMV707B.15-45 MHz transducer </td><td></td><td></td><td></td></tr><tr><td> Tec 3 Isofluran Vaporizer </td><td></td><td></td><td></td></tr><tr><td> Isofluran (2-chlor-2-(difluoromethoxy) -1,1,1-trifluor-ethan) </td><td></td><td></td><td></td></tr></tbody></table>

murine fetal echocardiography

Transgene mus viser abnormiteter i hjertets udvikling og funktion udgør et effektivt redskab til forståelsen af ​​de molekylære mekanismer, der ligger både normal hjerte-kar-funktion og patofysiologiske grundlag af menneskets hjertekarsygdomme. Føtal og perinatal død er et fælles træk, når man studerer genetiske ændringer, der påvirker hjerte-udvikling 1-3. For at undersøge den rolle, genetiske eller farmakologisk ændringer i den tidlige udvikling af hjertefunktionen er ultralydsscanning af den levende foster blevet et vigtigt redskab til tidlig opdagelse af abnormiteter og langsgående opfølgning. Noninvasiv ultralydsscanning er en ideel metode til påvisning og undersøgelse af medfødte misdannelser og indvirkningen på hjertefunktionen forud for dødsfaldet 4. Det giver mulighed for tidlig opdagelse af abnormiteter i den levende foster og progression af sygdommen kan følges i livmoderen med longitudinelle studier 5,6.Indtil for nylig hyppigt billeddannelse af føtale mus hjerter involveret invasive metoder. Fosteret skulle ofres for at udføre magnetisk resonans mikroskopi og elektronmikroskopi eller kirurgisk leveres til gennemlysning mikroskopi. En anvendelse af højfrekvente prober med konventionel 2-D og pulserende bølge Doppler imaging har vist sig at give målinger af hjertets sammentrækning og hjerte satser under fosterudviklingen med databaser af normale udviklingsmæssige ændringer nu tilgængelige 6-10. M-mode scanning endvidere vigtige funktionelle data, selv om de korrekte billeddiagnostiske fly er ofte vanskeligt at opnå. Højfrekvent ultralydsscanning af fosteret er forbedret 2-D opløsning og kan give gode oplysninger om den tidlige udvikling af hjerte-strukturer 11.

Watch this Scientific Journal Video about Murine Fetal Echocardiography at JoVE.com

Murine Fetal Echocardiography

Føtal og perinatal død er et fælles træk, når man studerer genetiske ændringer, der påvirker hjerte-udvikling. Højfrekvent ultralydsscanning har forbedret 2-D opløsning og kan give gode oplysninger om tidlig hjerte-udvikling og er en ideel metode til at detektere påvirkning af hjertets struktur og funktion forud for dødsfaldet.

Murin Føtal Ekkokardiografi

Transgenic mice displaying abnormalities in  cardiac development and function represent a powerful tool for the understanding  the molecular mechanisms ...

Research

JoVE Journal

Medicine

Transthoracic Echocardiography in Mice

In recent years, murine models have become the primary avenue for studying the molecular mechanisms of cardiac dysfunction resulting from changes in gene ...

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

Ischemia-reperfusion (IR) was surgically performed in murine hearts which were then subjected to repeated imaging to monitor temporal changes in ...

Murine Echocardiography and Ultrasound Imaging

Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics.1  ...

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Evaluering Teratogene Ændringer i en Zebrafisk Model af Føtalt Alkohol Eksponering

Fetal alcohol syndrome (FAS) is a severe manifestation of embryonic exposure to ethanol. It presents with characteristic defects to the face and organs, ...

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction

Føtal Ekkokardiografi og Pulserende-bølge Doppler Ultrasound i en kanin Model of Intrauterin vækst Begrænsning

Fetal intrauterine growth restriction (IUGR) results in abnormal cardiac function that is apparent antenatally due to advances in fetoplacental Doppler ...

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Isolation, Kultur og Imaging for Human Føtal pankreascelle Clusters

For almost 30 years, scientists have demonstrated that human fetal ICCs transplanted under the kidney capsule of nude mice matured into functioning ...

A Mouse Fetal Skin Model of Scarless Wound Repair

En mus Føtal Skin Model af scarless sårheling

Early in utero, but not in postnatal life, cutaneous wounds undergo regeneration and heal without formation of a scar. Scarless fetal wound healing occurs ...

Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery

Murine ekkokardiografi af Venstre Atrium, Aorta, og lungepulsåren

The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic ...

2D and 3D Echocardiography in the Axolotl (Ambystoma Mexicanum)

2D and 3D Echocardiography in the Axolotl Ambystoma Mexicanum

2D og 3D ekkokardiografi i Axolotl (Ambystoma Mexicanum)

Cardiac malfunction as a result of ischemic heart disease is a major challenge, and regenerative therapies to the heart are in high demand. A few model ...

Model Surgical Training: Skills Acquisition in Fetoscopic Laser Photocoagulation of Monochorionic Diamniotic Twin Placenta Using Realistic Simulators

Model kirurgiske uddannelse: Kompetenceudvikling i Fetoscopic Laser fotokoagulation af Monochorionic Diamniotic Twin moderkagen ved hjælp af realistiske simulatorer

Fetoscopic laser coagulation of arterio-venous anastomoses (AVA) in a monochorionic placenta is the standard of care for twin-twin transfusion syndrome ...