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DOI :
10.3791/4441-v
October 5th, 2012
Chapters
0:05
Title
1:20
Genomic DNA Fragmentation
2:02
Glucosyl Transfer: B-GT Reaction
2:49
Biotinylation Reaction: Click Chemistry
3:30
Capture of 5-hmC-containing DNA
4:51
Results: Selective Capture of 5-hmC from Genomic DNA
6:01
Conclusion
描述的是一个两步骤的标记过程中使用的β-葡糖基转移酶(β-GT)来传输的叠氮化物5-HMC-葡萄糖,然后通过点击化学生物素链接器为容易和密度独立富集转移。这种高效的和具体的标记方法使富集5 - HMC具有极低的背景和高通量表观映射的通过新一代测序。
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