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10.9K Views
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13:54 min
October 21st, 2012
DOI :
10.3791/4442-v
Chapters
0:05
Title
1:50
Generation of Inducible Cell Lines
6:19
Induction of Knockdown and Transfection
8:08
DAPI Assay in Transfected Cells
10:59
Results: Detection of E4orf4-induced Cell Death in Inducible Cell Lines
13:06
Conclusion
Summary
E4orf4誘導細胞死へのACFのクロマチンリモデリング因子の寄与を測定した。プロトコルはドキシサイクリン治療がACFユニットAcf1とSNF2hの条件付きノックダウンを誘導し、誘導細胞株でE4orf4誘導される細胞死を測定するためのDAPIアッセイを使用した細胞クローンの選択が含まれています。
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