Name: orthotopic mouse model of colorectal cancer
Uploaded: 2007-12-04T04:21:00
Description: Watch this Scientific Journal Video about Orthotopic Mouse Model of Colorectal Cancer at JoVE.com

<p>外科、カリフォルニア大学、サンフランシスコ<br />レイモンドシャヒーン、MD </p>

<p class="jove_step"> I.細胞の調製</p><ol><li>大腸がん細胞を培養中で成長し、時サブコン収穫されています。 </li><li>単一の細胞懸濁液をリン酸で調製される緩衝生理食塩水と氷上で保存。 </li></ol><p class="jove_step"> II。腫瘍の準備</p><ol><li>以前に確立された皮下大腸の腫瘍を持つマウスを安楽死されている。 </li><li>皮下腫瘍は​​無菌操作を用いて除去し、2〜3mmの部分に分かれています。 </li><li>腫瘍部分は氷の上に?...

<ol>
	<li>Heijstek, M. W., Kraneburg, O., Rinkes Borel, I. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+colorectal+cancer+and+liver+metastases.">Mouse models of colorectal cancer and liver metastases.</a> <em style='font-style: italic'>Dig Surg</em>. <b>22</b>, 16-25 (2005).</li><li>Kobaek-Larsen, M., Thorup, I., Diederichsen, A., Fenger, C., Hoitinga, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Review+of+colorectal+cancer+and+its+metastases+in+rodent+models:+comparative+aspects+with+those+in+humans.">Review of colorectal cancer and its metastases in rodent models: comparative aspects with those in humans.</a> <em style='font-style: italic'>Comp Med</em>. <b>50</b>, 16-26 (2000).</li><li>Wilmanns, C., Fan, D., O&apos;Brian, C. A., Bucana, C. D., Fidler, I. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+and+ectopic+organ+environments+differentially+influence+the+sensitivity+of+murine+colon+carcinoma+cells+to+doxorubicin+and+5-fluorouracil.">Orthotopic and ectopic organ environments differentially influence the sensitivity of murine colon carcinoma cells to doxorubicin and 5-fluorouracil.</a> <em style='font-style: italic'>Int J Cancer</em>. <b>52</b>, 98-104 (1992).</li><li>Kashtan, H., Rabau, M., Mullen, J. B., Wong, A. H. C., Roder, J. C., Shiptz, B., Stern, H. S., Gallinger, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intra-rectal+injection+of+tumor+cells:+a+novel+animal+model+of+rectal+cancer.">Intra-rectal injection of tumor cells: a novel animal model of rectal cancer.</a> <em style='font-style: italic'>Surg Oncol</em>. <b>1</b>, 251-256 (1992).</li></ol>

<p>マウス皮下腫瘍モデルを確立し、監視も簡単ですが、それはこのモデルは、大腸癌の元解剖学的部位を複製できないことは明らかである。微小環境の違いにより、皮膚の下に成長している大腸癌細胞は、それらの表現型を変更し、ほとんどの場合<sup>1,2の</sup>進行と転移に失敗することが示されている。実際には、治療に対する腫瘍の反応は、がん細胞が異所性（皮下）対同所場所<sup>3</sup>に注入されてい?...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Cell culture media and components</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>will vary depending on cell line used</td>
</tr>
<tr>
<td>Phosphate Buffered Saline</td>
<td>&nbsp;</td>
<td>BioWhitaker</td>
<td>17-512F</td>
<td>also sold by a number of other vendors</td>
</tr>
<tr>
<td>15 mL centrifuge tubes, polypropylene</td>
<td>&nbsp;</td>
<td>Corning</td>
<td>430790</td>
<td>&nbsp;</td>
<tr>
<td>6 well plates with lid</td>
<td>&nbsp;</td>
<td>Becton Dickinson Labware</td>
<td>353046</td>
<td>&nbsp;</td>
<tr>
<td>Iris forceps, straight, serrated tips</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>15914</td>
<td>best to have a pair of these instruments</td>
</tr>
<tr>
<td>Iris dissecting scissors, straight, sharp</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>14393</td>
<td>&nbsp;</td>
<tr>
<td>Needle Holder (regular)</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>14109</td>
<td>&nbsp;</td>
<tr>
<td>Needle Holder (Castroviejo)</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>14137</td>
<td>&nbsp;</td>
<tr>
<td>Cidex Plus</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>7364</td>
<td>instruments should be autoclaved periodically, but may also be sterilized by soaking in Cidex</td>
</tr>
<tr>
<td>6-0 or 7-0 suture</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>for cecum; courtesy of Department of Surgery, UCSF</td>
</tr>
<tr>
<td>3-0 or 4-0 suture</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>for abd wall; courtesy of Department of Surgery, UCSF</td>
</tr>
<tr>
<td>Anesthetic machine / Isoflurane</td>
<td>&nbsp;</td>
<td>J.A. Baulch and Associates</td>
<td>3206</td>
<td>also sold by a number of other vendors</td>
</tr>
<tr>
<td>Heating Pad, disposable, 6" x 6"</td>
<td>&nbsp;</td>
<td>Prism Technologies</td>
<td>20419</td>
<td>&nbsp;</td>
<tr>
<td>Tape, orange, 13 mm x 13 m</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>15901F</td>
<td>for restraining anesthetized mouse</td>
</tr>
<tr>
<td>Pro-Cord/Cordless Trimmer</td>
<td>&nbsp;</td>
<td>Oster</td>
<td>78997-010</td>
<td>for shaving mouse fur</td>
</tr>
<tr>
<td>Betadine</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>19-027132</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>2 x 2 gauze, 3-ply</td>
<td>&nbsp;</td>
<td>Johnson and Johnson</td>
<td>7635</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>Sterile Field, Barrier, 16" x 29"</td>
<td>&nbsp;</td>
<td>Johnson and Johnson</td>
<td>0905</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>Cotton tipped applicators, 6"</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>14-960-3Q</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>Syringe, 10 cc, Luer lock tip</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309604</td>
<td>&nbsp;</td>
<tr>
<td>Syringe, 1 cc, tuberculin, slip tip</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309602</td>
<td>&nbsp;</td>
<tr>
<td>27 1/2 G needle</td>
<td>&nbsp;</td>
<td>BD</td>
<td>305109</td>
<td>&nbsp;</td>
<tr>
<td>30 G needle</td>
<td>&nbsp;</td>
<td>BD</td>
<td>305106</td>
<td>&nbsp;</td>		</tbody>
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orthotopic mouse model of colorectal cancer

<p class="jove_content">従来の皮下腫瘍モデルでは、大腸癌を研究するための理想的とは言えません。彼らの自然な場所で成長してがん細胞を特徴と大腸癌の同所性マウスモデルは、、高忠実度で人間の病気を複製する。 2つの手法は、このモデルを確立するために使用することができます。両方の手法は類似しており、盲腸の露出のためのマウスの麻酔や開腹を必要とする。一つの技術は盲腸壁に大腸癌の細胞懸濁液の注入を含む。がん細胞が最初に、培養で増殖するときにサブコン収穫し、単一の細胞懸濁液として調製されています。細胞の小音量は漏れを避けるためにゆっくりと注入されます。他のテクニックは、盲腸に皮下腫瘍の一部の移植を含む。以前に確立された皮下大腸の腫瘍を持つマウスを安楽死され、腫瘍は無菌操作を用いて除去される。腫瘍部分は、別のマウスへの移植のための小さな断片に分割されています。移植前に、盲腸壁は軽く腫瘍細胞の浸潤を促進するために破損している。原発腫瘍と肝転移を開発する時間はテクニック、細胞株、および使用されるマウスの種によって異なります。この同所マウスモデルでは、大腸癌の自然な進行を勉強し、大腸癌に対する新しい治療薬をテストするのに便利です。</p>

Watch this Scientific Journal Video about Orthotopic Mouse Model of Colorectal Cancer at JoVE.com

Orthotopic Mouse Model of Colorectal Cancer

<p class="jove_content">盲腸に皮下腫瘍の一部の盲腸壁や移植に癌の細胞懸濁液の注入：2つのテクニックは、このモデルを確立するために使用することができます。このモデルでは、大腸癌の自然な進行を勉強し、大腸癌に対する新しい治療薬をテストするのに便利です。</p>

大腸癌の同所性マウスモデル

The traditional subcutaneous tumor model is less than ideal for studying colorectal cancer. Orthotopic mouse models of colorectal cancer, which feature ...

Research

JoVE Journal

Biology

Olfactory Behavioral Testing in the Adult Mouse

成体マウスでの嗅覚行動試験

The rodent olfactory system is of increasing interest to scientists, studied, in part, in systems biology because of its stereotyped, yet accessible ...

Chronic <em>Salmonella</em> Infected Mouse Model

Chronic Salmonella Infected Mouse Model

慢性<em>サルモネラ</em>感染マウスモデル

The bacterial infected mouse model is a powerful model system for studying areas such as infection, inflammation, immunology, signal transduction, and ...

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

胚盤胞からマウス栄養膜幹細胞の導出

Specification of the trophectoderm is one of the earliest differentiation events of mammalian development. The trophoblast lineage derived from the ...

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>生体</em>イメージングおよび卵巣癌の同所性マウスモデルにおける治療的処置<em>の</em>

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Flow Cytometry Purification of Mouse Meiotic Cells

マウス減数分裂細胞のフローサイトメトリーの精製

The heterogeneous nature of cell types in the testis and the absence of meiotic cell culture models have been significant hurdles to the study of the ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

人工多能性幹細胞に由来するマウスの作製

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

<em>En Face</em> Preparation of Mouse Blood Vessels

En Face Preparation of Mouse Blood Vessels

マウスの血管の<em>顔面</em>準備

Sections of paraffin embedded tissues are routinely used for studying tissue histology and histopathology. However, it is difficult to determine what the ...

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

トランス内部細胞のキメリズム率が高いため、胚性幹細胞の大量注入

In an effort to increase efficiency in the creation of genetically modified mice via ES Cell methodologies, we present an adaptation to the current ...

A Mouse Model of Intestinal Partial Obstruction

腸の部分的な閉塞のマウス モデル

Intestinal obstructions, that impede or block peristaltic movement, can be caused by abdominal adhesions and most gastrointestinal (GI) diseases including ...

Purification of Platelets from Mouse Blood

マウスの血液からの血小板の浄化

Platelets are purified from whole blood to study their functional properties, which should be free from red blood cells (RBC), white blood cells (WBC), ...