Name: orthotopic mouse model of colorectal cancer
Uploaded: 2007-12-04T04:21:00
Description: Watch this Scientific Journal Video about Orthotopic Mouse Model of Colorectal Cancer at JoVE.com

<p> Kirurgiska kliniken, University of California, San Francisco <br /> Raymond Shaheen, MD </p>

<p class="jove_step"> I. cellberedningen </p><ol><li> Kolorektal cancer celler odlas i kultur och skördas när subconfluent. </li><li> En enda cellsuspensionen är upprättad i fosfatbuffrad saltlösning och höll på is. </li></ol><p class="jove_step"> II. Tumör Förberedelser </p><ol><li> En mus med en tidigare fastställda subkutan kolorektal tumör avlivas. </li><li> Den subkutana tumören avlägsnas med hjälp av steril teknik och delas in i 2-3 mm bitar </li><li> Tumören bitar hålls i fosfatbuffrad saltlösning på is. </li></ol><p cl...

<ol>
	<li>Heijstek, M. W., Kraneburg, O., Rinkes Borel, I. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+colorectal+cancer+and+liver+metastases.">Mouse models of colorectal cancer and liver metastases.</a> <em style='font-style: italic'>Dig Surg</em>. <b>22</b>, 16-25 (2005).</li><li>Kobaek-Larsen, M., Thorup, I., Diederichsen, A., Fenger, C., Hoitinga, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Review+of+colorectal+cancer+and+its+metastases+in+rodent+models:+comparative+aspects+with+those+in+humans.">Review of colorectal cancer and its metastases in rodent models: comparative aspects with those in humans.</a> <em style='font-style: italic'>Comp Med</em>. <b>50</b>, 16-26 (2000).</li><li>Wilmanns, C., Fan, D., O&apos;Brian, C. A., Bucana, C. D., Fidler, I. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+and+ectopic+organ+environments+differentially+influence+the+sensitivity+of+murine+colon+carcinoma+cells+to+doxorubicin+and+5-fluorouracil.">Orthotopic and ectopic organ environments differentially influence the sensitivity of murine colon carcinoma cells to doxorubicin and 5-fluorouracil.</a> <em style='font-style: italic'>Int J Cancer</em>. <b>52</b>, 98-104 (1992).</li><li>Kashtan, H., Rabau, M., Mullen, J. B., Wong, A. H. C., Roder, J. C., Shiptz, B., Stern, H. S., Gallinger, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intra-rectal+injection+of+tumor+cells:+a+novel+animal+model+of+rectal+cancer.">Intra-rectal injection of tumor cells: a novel animal model of rectal cancer.</a> <em style='font-style: italic'>Surg Oncol</em>. <b>1</b>, 251-256 (1992).</li></ol>

<p> Även om musen subkutan tumörmodeller är lätta att fastställa och övervaka, är det tydligt att denna modell inte kan replikera den ursprungliga anatomiska platsen för kolorektal cancer. På grund av skillnaden i mikromiljö, har kolorektal cancer celler som växer under huden visat att förändra sin fenotyp och nästan alltid misslyckas med att framsteg och metastasera <sup>1,2.</sup> I själva verket kan tumören svarar på behandlingen varierar kraftigt beroende på om cancerceller implanteras i ett utomkvedshavandeskap (subkutant...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
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<tr>
<td>Cell culture media and components</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>will vary depending on cell line used</td>
</tr>
<tr>
<td>Phosphate Buffered Saline</td>
<td>&nbsp;</td>
<td>BioWhitaker</td>
<td>17-512F</td>
<td>also sold by a number of other vendors</td>
</tr>
<tr>
<td>15 mL centrifuge tubes, polypropylene</td>
<td>&nbsp;</td>
<td>Corning</td>
<td>430790</td>
<td>&nbsp;</td>
<tr>
<td>6 well plates with lid</td>
<td>&nbsp;</td>
<td>Becton Dickinson Labware</td>
<td>353046</td>
<td>&nbsp;</td>
<tr>
<td>Iris forceps, straight, serrated tips</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>15914</td>
<td>best to have a pair of these instruments</td>
</tr>
<tr>
<td>Iris dissecting scissors, straight, sharp</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>14393</td>
<td>&nbsp;</td>
<tr>
<td>Needle Holder (regular)</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>14109</td>
<td>&nbsp;</td>
<tr>
<td>Needle Holder (Castroviejo)</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>14137</td>
<td>&nbsp;</td>
<tr>
<td>Cidex Plus</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>7364</td>
<td>instruments should be autoclaved periodically, but may also be sterilized by soaking in Cidex</td>
</tr>
<tr>
<td>6-0 or 7-0 suture</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>for cecum; courtesy of Department of Surgery, UCSF</td>
</tr>
<tr>
<td>3-0 or 4-0 suture</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>for abd wall; courtesy of Department of Surgery, UCSF</td>
</tr>
<tr>
<td>Anesthetic machine / Isoflurane</td>
<td>&nbsp;</td>
<td>J.A. Baulch and Associates</td>
<td>3206</td>
<td>also sold by a number of other vendors</td>
</tr>
<tr>
<td>Heating Pad, disposable, 6" x 6"</td>
<td>&nbsp;</td>
<td>Prism Technologies</td>
<td>20419</td>
<td>&nbsp;</td>
<tr>
<td>Tape, orange, 13 mm x 13 m</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>15901F</td>
<td>for restraining anesthetized mouse</td>
</tr>
<tr>
<td>Pro-Cord/Cordless Trimmer</td>
<td>&nbsp;</td>
<td>Oster</td>
<td>78997-010</td>
<td>for shaving mouse fur</td>
</tr>
<tr>
<td>Betadine</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>19-027132</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>2 x 2 gauze, 3-ply</td>
<td>&nbsp;</td>
<td>Johnson and Johnson</td>
<td>7635</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>Sterile Field, Barrier, 16" x 29"</td>
<td>&nbsp;</td>
<td>Johnson and Johnson</td>
<td>0905</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>Cotton tipped applicators, 6"</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>14-960-3Q</td>
<td>may also be purchased at any medical supply store</td>
</tr>
<tr>
<td>Syringe, 10 cc, Luer lock tip</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309604</td>
<td>&nbsp;</td>
<tr>
<td>Syringe, 1 cc, tuberculin, slip tip</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309602</td>
<td>&nbsp;</td>
<tr>
<td>27 1/2 G needle</td>
<td>&nbsp;</td>
<td>BD</td>
<td>305109</td>
<td>&nbsp;</td>
<tr>
<td>30 G needle</td>
<td>&nbsp;</td>
<td>BD</td>
<td>305106</td>
<td>&nbsp;</td>		</tbody>
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orthotopic mouse model of colorectal cancer

<p class="jove_content">Den traditionella subkutana tumören modellen är mindre än idealisk för att studera kolorektal cancer. Orthotopic musmodeller av kolorektal cancer, som har cancerceller växer i sin naturliga plats, replikera mänskliga sjukdomar med hög trohet. Två tekniker kan användas för att fastställa denna modell. Båda teknikerna är snarlika och kräver mus anestesi och laparotomi för exponering av blindtarmen. En teknik innebär injektion av en kolorektal cancer cellsuspensionen i cecal väggen. Cancerceller är först odlas i kultur, skördas när subconfluent och förbereds som en enda cell suspension. En liten mängd celler injiceras långsamt för att undvika läckage. Den andra tekniken innebär transplantation av en bit av subkutan tumör på blindtarmen. En mus med en tidigare fastställda subkutan kolorektal tumör är euthanized och tumören avlägsnas med steril teknik. Tumören Stycket är indelat i små bitar för transplantation till en annan mus. Före transplantation är cecal väggen lätt skadade att underlätta tumörceller infiltration. Tiden för att utveckla primära tumörer och metastaser i levern kommer att variera beroende på tekniken, cellinje, och musen används arter. Denna orthotopic musmodell är användbar för att studera det naturliga förloppet av kolorektal cancer och testa nya behandlingsmetoder mot kolorektal cancer.</p>

Watch this Scientific Journal Video about Orthotopic Mouse Model of Colorectal Cancer at JoVE.com

Orthotopic Mouse Model of Colorectal Cancer

<p class="jove_content">Två tekniker kan användas för att fastställa denna modell: injektion av en cancercell suspensionen i cecal vägg eller transplantation av en bit av subkutan tumör på blindtarmen. Denna modell är användbar för att studera det naturliga förloppet av kolorektal cancer och testa nya behandlingsmetoder mot kolorektal cancer.</p>

Orthotopic musmodell av kolorektal cancer

The traditional subcutaneous tumor model is less than ideal for studying colorectal cancer. Orthotopic mouse models of colorectal cancer, which feature ...

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