Name: utilizing a cranial window to visualize the middle cerebral artery during endothelin-1 induced middle cerebral artery occlusion
Uploaded: 2013-02-22T12:00:00
Description: Watch this Scientific Journal Video about Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion at JoVE.com

Dette arbejde blev støttet af tilskud fra American Heart Association Greater Southeast Affiliate (09GRNT2060421), American Medical Association, og fra University of Florida klinisk og translationel Science Institute. Adam Mekka er et NIH / NINDS, NRSA predoctoral fyr (F30 NS-060.335). Robert Regenhardt fik predoctoral stipendium støtte fra University of Florida Multidisciplinary Training Program i Hypertension (T32 HL-083.810).

Denne protokol blev godkendt af Institutional Animal Care og Use Committee (IACUC) ved University of Florida og er i overensstemmelse med &quot;Vejledning for Pleje og anvendelse af forsøgsdyr&quot; (ottende udgave, National Academy of Sciences, 2011). Materialer <ol><li> Dyr: Otte uger gamle, Sprague Dawley hanrotter (Charles River Farms, Wilmington, MA, USA), som vejede 250-300 g på tidspunktet for kirurgi. </li><li> Anæstesi <ol class="lalpha"><li> Inhalationsanæstesi system (VetEqui...

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	<li>Levasseur, J. E., Wei, E. P., Raper, A. J., Kontos, A. A., Patterson, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detailed+description+of+a+cranial+window+technique+for+acute+and+chronic+experiments.">Detailed description of a cranial window technique for acute and chronic experiments.</a> Stroke. 6, 308-317 (1975).</li><li>Baumbach, G. L., Dobrin, P. B., Hart, M. N., Heistad, D. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanics+of+cerebral+arterioles+in+hypertensive+rats.">Mechanics of cerebral arterioles in hypertensive rats.</a> Circ. Res. 62, 56-64 (1988).</li><li>Regrigny, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+melatonin+on+rat+pial+arteriolar+diameter+in+vivo.">Effects of melatonin on rat pial arteriolar diameter in vivo.</a> Br. J. Pharmacol. 127, 1666-1670 (1999).</li><li>Mecca, A. P., O'Connor, T. E., Katovich, M. J., Sumners, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Candesartan+pretreatment+is+cerebroprotective+in+a+rat+model+of+endothelin-1-induced+middle+cerebral+artery+occlusion.">Candesartan pretreatment is cerebroprotective in a rat model of endothelin-1-induced middle cerebral artery occlusion.</a> Exp. Physiol. 94, 937-946 (2009).</li><li>Mecca, A. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerebroprotection+by+angiotensin-(1-7)+in+endothelin-1-induced+ischaemic+stroke.">Cerebroprotection by angiotensin-(1-7) in endothelin-1-induced ischaemic stroke.</a> Exp. Physiol. 96, 1084-1096 (2011).</li></ol>

Sammenfattende er det kraniel vindue fremstillingsteknik meget fleksibel, som den kan ændres til at imødekomme behovene hos mange eksperimenter med mindre modifikationer 4, 5. For eksempel har vi med succes overvåget cerebral blodgennemstrømning i bestemte MCA brancher bruger laser-doppler flowmetri at fokusere direkte på en cerebral arterie visualiseres gennem et kranie vindue (Mekka AP 2009 og 2011). Endvidere kan et lignende præparat med dura indsnit anvendes med topisk administration af vasoaktive f...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer (valgfrit) </td></tr><tr><td> Indånding anæstesi-system </td><td> VetEquip Inc., Pleasanton, CA, USA </td><td> 901.806 </td><td></td></tr><tr><td> Isofluran anæstesi </td><td> Baxter Pharmaceutics, Deerfield, IL, USA </td><td> 1001936060 </td><td></td></tr><tr><td> Små dyr stereotaktisk-system </td><td> David Kopf Instruments, Tujunga, CA, USA </td><td> 900 </td><td></td></tr><tr><td> Ikke-ruptur øre stænger, rotte </td><td> David Kopf Instruments, Tujunga, CA, USA </td><td> 957 </td><td></td></tr><tr><td> Rotte gas anæstesi hovedholder </td><td> David Kopf Instruments, Tujunga, CA, USA </td><td> 1929 </td><td></td></tr><tr><td> BAT-12 mikrosonde termometer </td><td> Verden Precision Instruments, Inc., Sarasota, FL, USA </td><td> BAT-12 </td><td&gt; </td></tr><tr><td> T / PUMP, Thermal tæppe </td><td> Gaymar Industries, Inc., Orchard Park, NY, USA </td><td> T / PUMP, TP600 </td><td></td></tr><tr><td> Metzenbaum Sakse </td><td> Verden Precision Instruments, Inc., Sarasota, FL, USA </td><td> 501.254 </td><td></td></tr><tr><td> Iris pincet </td><td> Verden Precision Instruments, Inc., Sarasota, FL, USA </td><td> 15.915 </td><td></td></tr><tr><td> Bulldog clamp retraktorer </td><td> Verden Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14119-G </td><td></td></tr><tr><td> 10 pi sprøjte 26-gaugue </td><td> Verden Precision Instruments, Inc., Sarasota, FL, USA </td><td> SGE010RNS </td><td></td></tr><tr><td> Bovie, høj temperatur cautery kit </td><td> Verden Precision Instruments, Inc., Sarasota, FL, USA </td><td> 500.392 </td><td></td></tr><tr><td> Rat tand tang 0,12 </td><td> Stotz </td><td> E1811 </td><td></td></tr><tr><td> Mikromotorer drill </td><td> Stoelting,Wood Dale, IL, USA </td><td> 51.449 </td><td></td></tr><tr><td> 0,8 mm rund bor bur </td><td> Roboz Surgical Instrument Co, Inc., Gaithersburg, MD, USA </td><td> RS-6280C-1 </td><td></td></tr><tr><td> STORZ Bonn suturering pincet </td><td> Bausch and Lomb, Inc., Rochester, NY, USA </td><td></td><td></td></tr><tr><td> Nylon sutur, størrelse 3,0 </td><td> Oasis, Mettawa, IL, USA </td><td> MV-663 </td><td></td></tr><tr><td> Vatpinde </td><td> Fisher Scientific, Pittsburg, PA, USA </td><td> 22-029-488 </td><td></td></tr><tr><td> Puralube øjensalve </td><td> Fisher Scientific, Pittsburg, PA, USA </td><td> NC0138063 </td><td></td></tr><tr><td> Elektriske hårklippemaskiner </td><td> Oster, Providence, RI, USA </td><td> 78005-301 </td><td></td></tr><tr><td> ET-1 fortyndedes til 80 μ M koncentration i PBS </td><td> American Peptide, Sunnyvale, CA, USA </td><td> 88-1-10A </td><td></td></tr><tr><td> Chlorhexidin, 2% </td&gt; <td> Agrilabs, St. Joseph, MO, USA </td><td> 1040, Rev 6-06, NAC No: 10580322 </td><td></td></tr><tr><td> Kirurgisk mikroskop </td><td> Seiler Instrument and Manufacturing, St. Louis, MO, USA </td><td> Evolution XR6 </td><td></td></tr><tr><td> Sony Handycam </td><td> Sony, Minato, Tokyo, Japan </td><td> HDR-SR12 </td><td></td></tr><tr><td> Fiberoptisk illuminator </td><td> TechniQuip Corp, Livermore, CA, USA </td><td> FO1-150 </td><td></td></tr><tr><td> VLC Media Player </td><td> (Paris, Frankrig) </td><td></td><td></td></tr><tr><td> Billede J-software </td><td> US National Institutes of Health, Bethesda, MA, USA </td><td></td><td></td></tr></tbody></table>

utilizing a cranial window to visualize the middle cerebral artery during endothelin-1 induced middle cerebral artery occlusion

Oprettelse af en kraniel vindue er en fremgangsmåde, der giver mulighed for direkte visualisering af strukturer på den kortikale overflade af hjernen 1-3. Denne teknik kan udføres på mange steder ligger over rotte-cerebrum, men lettest udføres ved at skabe en kraniektomi i de let tilgængelige frontale og parietale knogler. Oftest har vi anvendt denne teknik i kombination med endothelin-1 mellem-cerebral arterieokklusion model af iskæmisk slagtilfælde at måle ændringer af arteria cerebri media kardiameter, der opstår med injektion af endothelin-1 i hjerneparenkymet støder op til den proksimale MCA 4, 5. For at visualisere den proximale del af MCA under endothelin -1 induceret MCAO, bruger vi en teknik til at skabe en kraniel vindue gennem tindingebenet på den laterale del af rotte kraniet (figur 1). Cerebrale arterier kan visualiseres enten med dura intakte eller med dura indsnit og retraCTED. Mest almindeligt, forlader vi dura intakt under visualisering siden endothelin-1-induceret MCAO indebærer levering af karsammentrækkende peptid ind i hjernen parenkym. Dette omgår behovet for at incise dura mater direkte over de visualiserede skibe til lægemiddelafgivelse. Denne protokol vil beskrive, hvordan du opretter en kraniel vindue til at visualisere cerebrale arterier i en trinvis måde, samt hvordan man kan undgå mange af de potentielle faldgruber vedrørende denne metode.

Stillbilleder taget fra optagne video viser, at ændringen i cerebrale arterie diameter efter ET-1 injektion kan let forstås ved hjælp af den kraniale vindue teknik (fig. 2). Inden for få minutter af ET-1 injektion, vil fartøjet begynde at snøre. Til sidst skibene vil være vanskeligt at visualisere og hjernevæv bliver bleg. Efter ca 20 min virkningerne af ET-1 mindskes og skibene begynder at udvide sig, gradvist vendte tilbage til udgangsværdien diameter efter omkring 45 min. Ud over de mere ind...

Watch this Scientific Journal Video about Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion at JoVE.com

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

I denne artikel beskrives en metode til at visualisere rotte-cerebrale arterier gennem en kranial vindue ved hjælp tidsmæssige kraniektomi for at se proximale dele af den midterste cerebrale arterie (<strong&gt; Figur 1</strong&gt;). Denne alsidige fremgangsmåde kan kombineres med forskellige teknikker for lægemiddelafgivelse til at måle cerebral arterie reaktivitet<em&gt; In vivo</em&gt;.

Anvendelse af en Cranial vindue for at visualisere den midterste cerebrale arterie Under endothelin-1-induceret mellem-cerebral arterieokklusion

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

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