Name: multi-analyte biochip (mab) based on all-solid-state ion-selective electrodes (assise) for physiological research
Uploaded: 2013-04-18T08:00:00
Description: Watch this Scientific Journal Video about Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research at JoVE.com

我们想感谢美国宇航局天体生物学科学和技术仪器发展（ASTID）计划的资金支持（授权号码103498和103692），大风洛克伍德的大学Birck Nantechnology的中心在美国普渡大学人与生物圈设备的引线键合，和亨俊园CAD图纸的流动池室。

1。聚（3,4 -乙撑二氧噻吩）：聚（4 -苯乙烯磺酸钠）（PEDOT：PSS）的电化学解的H +和CO 3 2 -离子的制备<ol><li>加入70毫克聚（4 -苯乙烯磺酸钠）（Na +的PSS - ），直到完全分散（大约10秒）〜10毫升去离子（DI）水和涡。 </li><li> 10.7微升3,4-ethlyenedioxythiophene二氧噻吩（EDOT）加入到该溶液中，在1.1和旋涡，直到溶液完全混合。 </li></ol> 2。制备聚?...

<ol>
	<li>Migdalski, J., Bas, B., Blaz, T., Golimowski, J., Lewenstam, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Miniaturized+and+Integrated+Galvanic+Cell+for+the+Potentiometric+Measurement+of+Ions+in+Biological+Liquids.">A Miniaturized and Integrated Galvanic Cell for the Potentiometric Measurement of Ions in Biological Liquids.</a> J. Solid State Electrochem. 13, 149-155 (2009).</li><li>Buehler, M. G., Kounaves, S. P., Martin, D. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Designing+a+Water-quality+Monitor+with+Ion-selective-electrodes.">Designing a Water-quality Monitor with Ion-selective-electrodes.</a> 1, 331-338 (2001).</li><li>Adamchuk, V. I., Lund, E. D., Sethuramasamyraja, B., Morgan, M. T., Doberman, A., Marx, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+Measurement+of+Soil+Chemical+Properties+on-the-go+using+Ion-selective-electrodes.">Direct Measurement of Soil Chemical Properties on-the-go using Ion-selective-electrodes.</a> Journal Computers and Electronics in Agriculture. 48 (3), 272-294 (2005).</li><li>Oel&#946;ner, W., Hermann, S., Kaden, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemical+Sensors+and+Sensor+Module+for+Studying+Biological+Systems+in+Space+Vehicles.">Electrochemical Sensors and Sensor Module for Studying Biological Systems in Space Vehicles.</a> Aerospace Science and Technology. 1, 291-296 (1997).</li><li>Bobacka, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conducting+Polymer-based+Solid-state+Ion-selective+Electrodes.">Conducting Polymer-based Solid-state Ion-selective Electrodes.</a> Electroanalysis. 18 (1), 7-18 (2006).</li><li>Buck, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Ion Selective Electrodes in Analytical Chemistry. , (1980).</li><li>Nam, H., Cha, G. S., Yang, V. C., Ngo, T. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chapter+18.">Chapter 18.</a> Biosensors and their Applications. , (2000).</li><li>Anatova-Ivanova, S., Mattinen, U., Radu, A., Bobacka, J., Lewenstem, A., Migdalski, J., Danielewski, M., Diamond, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+Miniature+All-solid-state+Potentiometric+Sensing+System.">Development of Miniature All-solid-state Potentiometric Sensing System.</a> Sensors and Actuators B. 146, 199-205 (2010).</li><li>Michalska, A., Galuszkiewicz, A., Ogonowska, M., Ocypa, M., Maksymiuk, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PEDOT+Films:+Multifunctional+Membranes+for+Electrochemical+Ion+sensing.">PEDOT Films: Multifunctional Membranes for Electrochemical Ion sensing.</a> J. Solid State Electrochem. 8, 381-389 (2004).</li><li>Bard, A. J., Faulkner, L. R., ed, 2. n. d. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Electrochemical Methods: Fundamentals and Applications. , (2000).</li><li>Claussen, J. C., Artiles, M. S., McLamore, E. S., Mohanty, S., Shi, J., Rickus, J., Fisher, T. S., Porterfield, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemical+Glutamate+Biosensing+with+Naanocube+and+Nanosphere+Augmented+Single-walled+Carbon+Nanotube+Networks:+A+Comparative+Study.">Electrochemical Glutamate Biosensing with Naanocube and Nanosphere Augmented Single-walled Carbon Nanotube Networks: A Comparative Study.</a> J. Mater. 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MAB生物芯片由的ASSISEs顶上基于PEDOT-CP的共轭转导层上的Pt电极的组合，其中一个可测量的电信号传导的离子浓度还从ISM构造。甲稳定的电极电位是指由CP层和ISM层。这两个层也决定MAB和质量的测量的电信号（噪声，漂移）的工作寿命。 PEDOT作为传导层由于其离子和电子的属性（在它的氧化形式）是特别有吸引力的。 PEDOT具有高氧化还原电容的能力，以尽量减少导电电极极化效...

<table border="1">
		<tbody>
		 <tr>
			<td>Name of the items</td>
			<td>Company</td>
			<td>Catalog number</td>
			<td>Comments </td>
		 </tr>
		 <tr>
			<td>3,4-Ethylenedioxythiophene</td>
			<td>Sigma-Aldrich</td>
			<td>483028</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Poly(sodium 4-styrenesulfonate)</td>
			<td>Sigma-Aldrich</td>
			<td>243051</td>
			<td></td>
		 </tr>
		 <tr>
			<td>EC epsilon galvanostat/potentiostat</td>
			<td>Bioanalytical Systems Inc.</td>
			<td>e2P</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Saturated Ag/AgCl reference electrode</td>
			<td>Bioanalytical Systems Inc.</td>
			<td>MF-2052</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Pt gauze</td>
			<td>Alfa Aesar</td>
			<td>10283</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium ferricyanide</td>
			<td>Sigma-Aldrich</td>
			<td>P-8131</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium nitrate</td>
			<td>J.T. Baker</td>
			<td>3190-01</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium bicarbonate</td>
			<td>Mallinckrodt/ Macron</td>
			<td>7412-12</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sodium carbonate</td>
			<td>Sigma-Aldrich</td>
			<td>S-7127</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Calcium chloride</td>
			<td>J.T. Baker</td>
			<td>1311-01</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Potassium chloride</td>
			<td>Sigma-Aldrich</td>
			<td>P9541</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Calcium sulphate</td>
			<td>Sigma-Aldrich</td>
			<td>237132</td>
			<td></td>
		 </tr>
		 <tr>
			<td>C3 cell stand</td>
			<td>Bioanalytical Systems Inc.</td>
			<td>EF-1085</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Flow-cell chip holder</td>
			<td></td>
			<td></td>
			<td>Custom, courtesy of NASA Ames</td>
		 </tr>
		 <tr>
			<td>Flow-cell electrical fixture</td>
			<td></td>
			<td></td>
			<td>Custom, courtesy of NASA Ames </td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>Table 2. Specific reagents and equipment.</td>
		 </tr>
		</tbody>
 </table>

multi-analyte biochip (mab) based on all-solid-state ion-selective electrodes (assise) for physiological research

一个芯片上实验室（LOC）应用在环保，生物医药，农业，生物，航天科研需要的离子选择性电极（ISE），可以承受长时间储存在复杂的生物介质1-4。一种全固态离子选择性电极（ASSISE）是上述的应用特别有吸引力的。电极应具有以下良好的特性：施工方便，维护成本低，和（潜在的）的小型化，可以进行批量处理。甲微加工ASSISE的用于量化的Ca 2 +，H +，CO 3 2 -离子的构建。它由一个贵金属电极层（ 例如铂），转导层和一个离子选择膜（ISM）的层。的转导层的功能的转导的浓度依赖型的离子选择性膜的化学势成可测量的电信号。 Ť他一生的ASSISE被发现依赖于保持电位的导电性层/膜界面5-7。要延长工作寿命ASSISE，在界面层，从而保持稳定的潜力，我们利用银/氯化银（Ag / AgCl电极的导电聚合物（CP），聚（3,4 -亚乙基二氧噻吩）（PEDOT）7-9）作为振子层。我们构建了ASSISE在一个芯片上的实验室的格式，我们称之为多分析物的生物芯片（MAB）（ 图1）。 校准测试解决方案表明，人与生物圈计划可监测pH值（业务范围pH值4-9），CO 3 2 - （测量范围0.01毫米- 1毫米），和Ca 2 +（对数线性范围为0.01毫米至1毫米）。人与生物圈计划海藻介质，存储在近一个月后，pH值，提供了近能斯特斜率响应。的碳酸酯的生物芯片显示类似于常规的离子选择性电极的电位的配置文件。生理学一例出现的测量值来监视系统的模型，生物活性小球藻 。 人与生物圈计划传达尺寸，多功能的优势，复分析物的感应能力，使得它适用于许多密闭监控的情况下，地球上或空间。 生物芯片的设计与实验方法生物芯片是10 x 11毫米的尺寸和有9 ASSISEs指定为工作电极（WES）和Ag / AgCl参考电极（RES）。每个工作电极（WE）的直径为240微米，平均间距为1.4毫米从RE的，直径为480微米的。这些电极连接到电接触垫的尺寸为0.5毫米×0.5毫米。的示意图如图2所示。 循环伏安法（CV）和恒电流沉积方法用于electropolymerize使用生物分析的PEDOT膜人系统公司（BASI）C3细胞支架（ 图3）。 PEDOT薄膜的反离子量身定制，以适应待测离子的利益。甲PEDOT与聚（苯乙烯磺酸）抗衡离子（PEDOT / PSS）是利用H +和CO 3 2 - ，，而硫酸钠（加入到该溶液中的CaSO 4）的是，利用的Ca 2 +。 PEDOT涂层的电化学性能，我们的分析中使用CVS氧化还原活性的解决方案（ 即 2毫米铁氰化钾（K 3的Fe（CN）6））。简历简介基于，兰德尔斯Sevcik分析被用来确定的有效表面积10。旋涂在1,500 rpm〜2微米厚的离子选择性膜（ISMS）的人与生物圈计划工作电极​​（WES）用于铸造。 人与生物圈被包含在一个微流体的流动池室充满了150微升体积的海藻介质接触垫电连接到的BASI系统（ 图如图4）。 小球藻光合活性的监测环境光线和黑暗条件下。

<html>的循环伏安图（CV）的PEDOT：PSS和其对应的还原峰电流（I P）与扫描速度（V 1/2）的一个例子示于图5a和5b分别。 PEDOT： 硫酸钙在不同的扫描率和它的还原峰电流将不会显示。使用兰德尔斯-Sevcik分析如图10所示，有效的表面区域的固体接触的PEDOT：PSS和PEDOT：离子选择性膜的CaSO 4没有被发现，分别为4.4×10 -11?...

Watch this Scientific Journal Video about Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research at JoVE.com

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

全固态离子选择性电极（ASSISEs）的建造由导电聚合物（CP）传感器提供几个月的功能寿命在液体介质中。在这里，我们描述了在实验室上的一个芯片格式ASSISEs的制造和校准过程。证明ASSISE长时间储存​​后，在复杂的生物媒体一直保持着近能斯特斜率曲线。

多分析物生物芯片（MAB），基于全固态离子选择性电极（ASSISE）的生理研究

Lab-on-a-chip (LOC) applications in environmental, biomedical, agricultural, biological, and spaceflight research require an ion-selective electrode (ISE) ...

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Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

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