Name: cardiac stress test induced by dobutamine and monitored by cardiac catheterization in mice
Uploaded: 2013-02-10T14:00:00
Description: Watch this Scientific Journal Video about Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice at JoVE.com

我们感谢的Helio公司萨尔加多博士，雷纳塔Lataro和毛罗奥利维拉，医学院的里贝朗普雷图，巴西圣保罗大学和马斯特里赫特大学，心血管研究所马斯特里赫特，本詹森博士，在设置过程中的慷慨援助。 这项工作是支持的FONDECYT授出N°11090114 SDC

协议的Facultad的MEDICINA CLINICA ALEMANA省大学与发展的伦理委员会批准。 一，准备多巴酚丁胺输注<ol><li>将10毫克多巴酚丁胺溶解在20毫升无菌馏分水，为了获得500微克/毫升多巴酚丁胺的储备液。分装并储存于-20°C。这种解决方案可以使用至少3个月。 </li><li>解冻多巴酚丁胺储备溶液的等分试样，在室温下。 </li><li>稀释多巴酚丁胺原液在无菌的0.9％NaCl溶液中，以获得的多巴酚丁?...

<ol>
	<li>Hart, C. Y., Burnett, J. C., Redfield, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+avertin+versus+xylazine-ketamine+anesthesia+on+cardiac+function+in+normal+mice.">Effects of avertin versus xylazine-ketamine anesthesia on cardiac function in normal mice.</a> Am. J. Physiol. Heart Circ. Physiol. 281, H1938-H1945 (2001).</li><li>Janssen, B. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+anesthetics+on+systemic+hemodynamics+in+mice.">Effects of anesthetics on systemic hemodynamics in mice.</a> Am. J. Physiol. Heart Circ. Physiol. 287, H1618-H1624 (2004).</li><li>Lorenz, J. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+practical+guide+to+evaluating+cardiovascular,+renal,+and+pulmonary+function+in+mice.">A practical guide to evaluating cardiovascular, renal, and pulmonary function in mice.</a> Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, R1565-R1582 (2002).</li><li>Lorenz, J. N., Robbins, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+of+intraventricular+pressure+and+cardiac+performance+in+the+intact+closed-chest+anesthetized+mouse.">Measurement of intraventricular pressure and cardiac performance in the intact closed-chest anesthetized mouse.</a> Am. J. Physiol. 272, H1137-H1146 (1997).</li><li>Nemoto, S., DeFreitas, G., Carabello, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+catheterization+technique+in+a+closed-chest+murine+model.">Cardiac catheterization technique in a closed-chest murine model.</a> Contemp. Top. Lab Anim. Sci. 42, 34-38 (2003).</li><li>Daniels, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impaired+cardiac+functional+reserve+in+type+2+diabetic+db/db+mice+is+associated+with+metabolic,+but+not+structural,+remodelling.">Impaired cardiac functional reserve in type 2 diabetic db/db mice is associated with metabolic, but not structural, remodelling.</a> Acta Physiol. (Oxf). 200, 11-22 (2010).</li><li>De Celle, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+structural+and+functional+consequences+of+cardiac+ischaemia-reperfusion+injury+in+vivo+in+mice.">Long-term structural and functional consequences of cardiac ischaemia-reperfusion injury in vivo in mice.</a> Exp. Physiol. 89, 605-615 (2004).</li><li>Huntgeburth, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transforming+growth+factor+beta+oppositely+regulates+the+hypertrophic+and+contractile+response+to+beta-adrenergic+stimulation+in+the+heart.">Transforming growth factor beta oppositely regulates the hypertrophic and contractile response to beta-adrenergic stimulation in the heart.</a> PLoS One. 6, e26628 (2011).</li><li>Reddy, A. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+function+in+young+and+old+Little+mice.">Cardiac function in young and old Little mice.</a> J. Gerontol. A. Biol. Sci. Med. Sci. 62, 1319-1325 (2007).</li><li>Christoffersen, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+lipid+accumulation+associated+with+diastolic+dysfunction+in+obese+mice.">Cardiac lipid accumulation associated with diastolic dysfunction in obese mice.</a> Endocrinology. 144, 3483-3490 (2003).</li></ol>

多巴酚丁胺诱导的心脏压力测试通过心导管检查和监控是艰苦的。尽管如此，这里的协议描述的训练用短的时间内，在一个实验中，最后约一小时，它是可能的，以评估六个血流动力学参数。 这里提出的协议的关键步骤是血管插管。关于颈动脉插管，执行切口应足够深，以打破三个组织层的动脉，和足够大，以允许通过的导管。关于颈静脉插管，而出血的危险性是低的，静?...

<table border="1"><tbody><tr><td></td><td></td><td></td><td> 试剂 </td></tr><tr><td> PE-50/10 </td><td>华纳仪器</td><td> 64-0752 </td><td></td></tr><tr><td>丝线6/0 HR17 </td><td>塔古姆</td><td> SN0713K </td><td></td></tr><tr><td> xylacin 20毫克/毫升</td><td>化验所Centrovet </td><td></td><td></td></tr><tr><td>氯胺酮100毫克/毫升</td><td>拖动制药</td><td></td><td></td></tr><tr><td> 0.9％氯化钠</td><td>实验室桑德森SA </td><td></td><td></td></tr><tr><td>盐酸多巴酚丁胺</td><td> Sigma-Aldrich公司</td><td> D0676 </td><td></td></tr><tr><td>注射器U-100胰岛素29G所述½“ </td><td>泰尔茂医疗器械有限公司</td><td></td><td></td></tr><tr><td>钳解剖微型11.5厘米风格7 </td><td>劳顿MEDIZINTECHNIK </td><td> 09-0959 </td><td></td></tr><tr><td>格雷夫钳CVD0.7毫米7厘米</td><td>劳顿M​​edizintechnik的</td><td> 62-0263 </td><td></td></tr><tr><td>夹Dieffenbach斗牛犬氯STR 38毫米</td><td>劳顿M​​edizintechnik的</td><td> 60-010 </td><td></td></tr><tr><td> Vannas剪刀8厘米STR FH </td><td>劳顿M​​edizintechnik的</td><td> 63-1400 </td><td></td></tr><tr><td></td><td></td><td></td><td> 设备 </td></tr><tr><td> SPR-671 MIKRO笔尖压力导管</td><td>米勒仪器</td><td> 840-6719 </td><td></td></tr><tr><td>压力控制单元PCU-2000 </td><td>米勒仪器</td><td> 880-0129 </td><td></td></tr><tr><td> PowerLab系统4/30 </td><td>的ADInstruments Pty有限公司</td><td> ML866 </td><td></td></tr><tr><td> LabChartPro 7 </td><td>的ADInstruments Pty有限公司</td><td> MLU260 / 7 </td><td></td></tr><tr><td> Legato200输液泵</td><td> KdScientific </td><td> KD-KDS210P </td><td></td></tr><tr><td> TCAT-2LV温控器，恒温加热板</td><td> PhysiTemp仪器公司</td><td></td><td></td></tr><tr><td>医用氧气供应</td><td> Indura </td><td></td><td></td></tr><tr><td>直肠探头</td><td>的ADInstruments Pty有限公司</td><td> MLT1404 </td><td></td></tr><tr><td>三目显微镜，同轴照明</td><td> LW科学</td><td> Z2B-TRI-ETNE，ILP-1502-LTS1，ILP-1502-DGGF </td><td></td></tr></tbody></table>

cardiac stress test induced by dobutamine and monitored by cardiac catheterization in mice

多巴酚丁胺是更高的亲和在心脏（β1）比在动脉（β2）受体表达的受体表达的β-肾上腺素能受体激动剂。当全身给药时，它增加心脏的需求。因此中，多巴酚丁胺不能控制异常的节奏潜在风险的梗死或缺血区。 无论是ecocardiography或心导管检查，心脏负荷试验过程中可以进行监测心脏功能。后者是一种侵入性，但更精确和信息，前者的技术。 多巴酚丁胺诱导的心脏压力测试和监控完成这里所描述的心导管检查允许的话，在一个单一的实验，测量血流动力学参数如下：心脏心率（HR），收缩压，舒张压，舒张末压，最大正压力（DP / dt（下标max））和最大的负面新闻：URE发展（dP / dt的分钟 ），在基准条件下增加剂量多巴酚丁胺。 正如预期的那样，在正常小鼠中，我们观察到的多巴酚丁胺的剂量相关的增加，HR，dp / dt 最大值和dP / dt 分钟 。此外，在最高剂量测试（12毫微克/克/分钟），高脂肪的饮食诱导的肥胖小鼠的心功能失代偿被揭穿。

动脉压信号被定义的收缩压和舒张压。当该压力传感器是左心室内，其压力（LVP）波形的特征在于由下降到零的舒张压和心室收缩（ 图1）的左心房收缩前的外观。在基准条件下，氯胺酮-甲苯噻嗪麻醉的正常小鼠HR为280±24，P 最大为107±8，P 5 分钟 ±1，EDP 14±2，dP / dt的最大值为6081±365和dP / dt 分钟 5230±526。 在图2中可?...

Watch this Scientific Journal Video about Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice at JoVE.com

Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice

我们描述的协议来执行的心脏压力测试在正常小鼠的心脏导管插入术多巴酚丁胺诱导和监控。此外，我们揭露其应用到亚临床心脏疾病，高脂饮食诱导的肥胖小鼠。

多巴酚丁胺诱导的心脏压力测试和监控通过在小鼠心脏导管插入术

Dobutamine is a &beta;-adrenergic agonist with an affinity higher for receptor expressed in the heart (&beta;1) than for receptors expressed in the ...

Research

JoVE Journal

Medicine

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

右心室结构和功能的肺动脉缩窄经胸超声小鼠模型的评估

Emerging clinical data support the notion that RV dysfunction is critical to the pathogenesis of cardiovascular disease and heart failure1-3. Moreover, ...

科研

医学

Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse

心脏异常的实验性自身免疫性心肌炎的无创性评估磁共振成像显微镜在鼠标

Myocarditis is an inflammation of the myocardium, but only ~10% of those affected show clinical manifestations of the disease. To study the immune events ...

Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats

大鼠使用超声技术药物引起的肾血流动力学障碍的早期检测

The kidney normally functions to maintain hemodynamic homeostasis and is a major site of damage caused by drug toxicity. Drug-induced nephrotoxicity is ...

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect

心导​​管在小鼠测量压力体积的关系：调查的影响鲍迪奇

Animal models that mimic human cardiac disorders have been created to test potential therapeutic strategies. A key component to evaluating these ...

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

心肌梗死新生小鼠，心肌再生的模型

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and ...

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Circumlimbal 缝合法诱导大鼠和小鼠青光眼模型的建立

The circumlimbal suture is a technique for inducing experimental glaucoma in rodents by chronically elevating intraocular pressure (IOP), a well-known ...

Isolation of Atrial Myocytes from Adult Mice

从成年小鼠分离心膜肌细胞

The electrophysiological properties of atrial myocytes importantly affect overall cardiac function. Alterations in the underlying ionic currents ...

Biventricular Assessment of Cardiac Function and Pressure-Volume Loops by Closed-Chest Catheterization in Mice

小鼠闭胸导管插入术对心功能和压力-容积环的双心室评估

Assessment of cardiac function is essential to conduct cardiovascular and pulmonary-vascular preclinical research. Pressure-volume loops (PV loops) ...

Cardiac Response to &#946;-Adrenergic Stimulation Determined by Pressure-Volume Loop Analysis

Cardiac Response to ÃƒÅ½Ã‚Â²-Adrenergic Stimulation Determined by Pressure-Volume Loop Analysis

通过压力-体积环路分析确定心脏对β肾上腺素能刺激的反应

Determination of the cardiac function is a robust endpoint analysis in animal models of cardiovascular diseases in order to characterize effects of ...

A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion

临时双性肾动脉闭塞的急性肾损伤大型动物模型

Acute kidney injury (AKI) is associated with higher risk for morbidity and mortality post-operatively. Ischemia-reperfusion injury (IRI) is the most ...