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DOI :
10.3791/50066-v
December 17th, 2012
Chapters
0:05
Title
1:18
Preparation of Cells and Treatment with Translation Inhibitors
2:29
Digitonin Extraction and Cell Fixation
4:47
Fluorescent In situ Hybridization
6:21
Washing and Mounting the Stained Samples
8:08
Placental Alkaline Phosphatase and Cytochrome P450-8B1 mRNA ER-association
9:50
Conclusion
ここでは、哺乳類の組織培養細胞に小胞体関連mRNAを可視化する方法を説明します。この手法は、蛍光灯に続く細胞質の内容を削除するジギトニンと形質膜の選択的透過性上昇を伴うその場でハイブリダイゼーション。
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