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DOI :
10.3791/50070-v
•
9:29 min
February 1st, 2013
Chapters
0:05
Title
2:09
Isolation of Mononuclear Cells from PB and UCB
4:03
Electroporation of MNC
5:59
FACS Analysis of CAR Expression (Day 1)
6:32
Preparation of aAPC (Clone #4) on Day 1
7:01
aAPC-mediated Stimulation of CAR+ T Cells (Day 1)
7:33
Results: SB System Coupled with Co-culture on aAPC Generates CD19-specific CAR+ T Cells
8:44
Conclusion
3:04
Preparation of T Cells for Electroporation
CD19特異的キメラ抗原受容体(CAR)を発現するT細胞は、私たちのファースト·イン·ヒトの遺伝子治療の臨床試験におけるB細胞悪性腫瘍の治験治療として注入される。我々は、使用して、T細胞の遺伝子改変を記述眠れる森の美女(SB)のデザイナー、CD19 +人工抗原提示細胞上のCD19特異的CARと選択的伝播を紹介するシステム。
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