Name: antigens protected functional red blood cells by the membrane grafting of compact hyperbranched polyglycerols
Uploaded: 2013-01-02T12:00:00
Description: Watch this Scientific Journal Video about Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols at JoVE.com

资助这项研究是由加拿大血液服务（CBS）和加拿大研究所（CIHR）健康科学的研究合作基金。作者感谢的LMB高分子枢纽的UBC血液研究中心的研究设施的使用。基础设施的支持，加拿大创新基金会（CFI）和迈克尔史密斯健康研究基金会（MSFHR）。河Chapanian是一个收件人（CIHR / CBS）在输血科学博士后奖学金和收件人的MSFHR研究实习生博士后奖学金。 JN Kizhakkedathu是一个收件人的MSFHR生涯研究学者奖。

A.超支化的聚甘油改造（SS-HPG） <ol><li>地点冻干HPG 60 kDa的（0.5克，0.0083毫摩尔）在一个圆底烧瓶中，并真空下干燥过夜，在90℃下</li><li>冷藏烧瓶至室温，并溶解在无水吡啶（3ml）中的干燥的HPG。 </li><li>若要官能约8个羟基基团与羧基基团上HPG，添加催化量的二甲基氨基吡啶（5毫克/毫升的吡啶溶液一滴）的HPG溶液。到该混合物中，加入琥珀酸酐，（0.0067克，0.0664毫摩尔）溶解在0.5毫升吡啶在10?...

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万能供血者红细胞有很大的潜力，在提高血液的输血治疗的有效性和安全性。红细胞也被认为是有前途的药物运输车辆，由于其长循环和固有的生物相容性，14日，15。本文提出的实验评估丘脑-垂体-性腺修改红细胞在体外的特点。 体外性能和丘脑-垂体-性腺修改红细胞在体内循环，本集团已在最近8，11。 Dextran500K/PEG8K双水相体系中，补充盐和钠磷酸盐，红细胞...

<table border="1"><tbody><tr><td>环氧丙醇</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td>三羟甲基丙烷</td><td> Fluka公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td>甲醇钾</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td>无水吡啶</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td> 4 - 二甲氨基吡啶</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td>丁二酸酐</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td>丙酮</td><td> Fisher Scientific则</td><td></td><td> （ON，加拿大） </td></tr><tr><td>无水二甲基甲酰胺</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td> N-羟基琥珀酰亚胺</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td> N，N&#39;-二异丙基碳二亚胺</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td> MTS卡</td><td></td><td></td><td>高输入系统（MTS）卡（FL，USA） </td></tr><tr><td>右旋糖酐500 kDa的</td><td> Pharmacia精细化学公司</td><td></td><td> （瑞典） </td></tr><tr><td> PEG 8 kDa的</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td> FITC单克隆抗恒河猴D（RhD血型） </td><td>商Biodiagnostics </td><td></td><td> （宾夕法尼亚州，美国） </td></tr><tr><td> PE单克隆抗-CD47 </td><td> BD Biosciences公司</td><td></td><td> （新泽西州，美国） </td></tr><tr><td>德拉布坎试剂</td><td> Sigma Aldrich公司</td><td></td><td> （ON，加拿大） </td></tr><tr><td></td><td></td><td></td><td> 表化学品和试剂，用于嫁接丘脑-垂体-性腺聚合物RBC膜并对其进行分析。 </td></tr></tbody></table>

antigens protected functional red blood cells by the membrane grafting of compact hyperbranched polyglycerols

红细胞（RBC）输血是用于治疗急性和慢性的医疗问题，如重型地中海贫血和镰状细胞贫血1-3一些至关重要的。由于众多的RBC表面上的（〜308已知抗原4），在慢性输血治疗的患者发展由于丢失如同输血红细胞4，5的轻微上的抗原的同种抗体的抗原的存在下。接枝亲水性聚合物，如聚乙二醇（PEG）和超支化聚甘油（HPG）的形成，RBC膜排除层上防止表面抗原的抗体的相互作用，而不会影响通过小分子如氧，葡萄糖，和离子3。目前，没有一种方法是可用于普遍的红血供体细胞的产生部分是因为大量抗原（基于蛋白质和碳水化合物）的存在下对RBC表面和d提出严峻的挑战才有发展这种方法将大大提高输血的安全性，并显着提高红细胞的可用性和使用。在这份报告中，提出的实验，是用来开发抗原保护功能红细胞由HPG及其表征膜接枝。 HPGs高度生物相容性紧凑的聚合物6，7，并预期位于小区内的的糖萼围绕的脂质膜8，9和掩模RBC表面抗原10，11。

迷彩恒河猴D抗原和CD47 RBC表面蛋白的流式细胞仪定量，使用荧光标记的单克隆抗体，和具有代表性的结果在图1中给出。在HPG-接枝红细胞的情况下（灰色），该信号的强度降低（峰转移至左）相比，控制红细胞（红色和绿色），表示表示掩蔽的表面蛋白的细胞表面结合的抗体减少。 <img src="/files/ftp_upload/50075/50075fig1.jpg" alt="图1" fo:content-...

Watch this Scientific Journal Video about Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols at JoVE.com

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

细胞膜的变形例的红血细胞（红细胞），超支化聚甘油（HPG）。修改红细胞进行了表征双水相分割，渗透脆性和补体介导的溶解。使用流式细胞仪和微的打字系统（MTS）的血液分型卡的表面蛋白抗原的伪装进行了评估。

紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞

Red blood cell (RBC) transfusion is vital for the treatment of a number of acute and chronic medical problems such as thalassemia major and sickle cell ...

Research

JoVE Journal

Immunology and Infection

Depletion of Specific Cell Populations by Complement Depletion

补消耗的特定细胞群的枯竭

The purification of immune cell populations is often required in order to study their unique functions.  In particular, molecular approaches such as ...

科研

免疫与感染

Isolation of Mouse Peritoneal Cavity Cells

小鼠腹膜腔细胞的分离

The peritoneal cavity is a membrane-bound and fluid-filled abdominal cavity of mammals, which contains the liver, spleen, most of the gastro-intestinal ...

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

特定细胞群的净化荧光激活细胞分选（FACS）

Experimental and clinical studies often require highly purified cell populations.  FACS is a technique of choice to purify cell populations of known ...

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

人外周血NK细胞的扩展，纯化和功能评估

Natural killer (NK) cells play an important role in immune surveillance against a variety of infectious microorganisms and tumors. Limited availability of ...

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

定量检测抗体诱导补体激活的方法对红血细胞

Antibodies against red blood cells (RBCs) can lead to complement activation resulting in an accelerated clearance via complement receptors in the liver ...

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

在髓系细胞动态实时成像<em&gt;装甲运兵车<sup&gt;最小/ +</sup</em&gt;肠道肿瘤通过旋转盘共聚焦显微镜

Myeloid cells are the most abundant immune cells within tumors and have been shown to promote tumor progression. Modern intravital imaging techniques ...

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

与碘酸雪夫染色检测糖原的外周血单个核细胞

Periodic acid Schiff (PAS) staining is an immunohistochemical technique used on muscle biopsies and as a diagnostic tool for blood samples. ...

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

抗原驱动结肠炎模型的开发由抗原呈递细胞T细胞抗原研究的介绍

Inflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological ...

Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens

利用X射线晶体学，生物物理学，和功能分析，以确定管理机制T细胞癌抗原的受体识别

Human CD8+ cytotoxic T lymphocytes (CTLs) are known to play an important role in tumor control. In order to carry out this function, the cell ...

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率

The efficient transduction of specific genes into natural killer (NK) cells has been a major challenge. Successful transductions are critical to defining ...