Name: environmentally-controlled microtensile testing of mechanically-adaptive polymer nanocomposites for ex vivo characterization
Uploaded: 2013-08-20T08:55:55
Description: Watch this Scientific Journal Video about Environmentally-controlled Microtensile Testing of Mechanically-adaptive Polymer Nanocomposites for ex vivo Characterization at JoVE.com

Dette arbejde blev støttet af Institut for Biomedical Engineering ved Case Western Reserve University gennem både lab nystartede fonde (J. Capadona) og Medtronic Graduate Fellowship (K. Potter). Yderligere finansiering på denne forskning blev støttet delvist af NSF tilskud ECS-0621984 (C. Zorman) Case Alumni Association (C. Zorman), Department of Veterans Affairs gennem en Merit anmeldelse Award (B7122R), samt Advanced Platform Technology Center (C3819C).

1.. Prøveforberedelse <ol><li> Forbered PVAc-NC film af tykkelse i området fra 25 til 100 um med en opløsning støbning og komprimering teknik 10-12. </li><li> Klæbe filmen til en silicium wafer ved opvarmning på en varm plade til to minutter ved 70 ° C (over glasovergangstemperaturen) for at fremme intim kontakt mellem filmen og skiven. Dette trin sikrer, at det fremstillede film stadig flad og fast til Si wafer, der er nødvendig for plane micromachining processer. </li><li> Mønster filmen til fors...

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Udviklingen af ​​implanterbare biomedicinske microelectromechanical systemer (bioMEMS) for at interagere med biologiske systemer er motiverende udvikling af nye materialer med højt tilpassede egenskaber. Nogle af disse materialer er designet til at udvise en ændring i materialeegenskaber som reaktion på en stimulus findes i det fysiologiske miljø. Et nyligt udviklet klasse af materialer reagerer på tilstedeværelsen af hydrogen bindingsdannende væsker (fx vand) og forhøjede temperaturer for at reduce...

<table border="1">
	<tbody>
		<tr>
			<td>Name of Reagent/Material</td>
			<td>Company</td>
			<td>Catalogue Number</td>
			<td>Comments</td>
		</tr>
		<tr>
			<td>Silicon wafer</td>
			<td>University Wafer</td>
			<td>&nbsp;</td>
			<td>Mechanical grade</td>
		</tr>
		<tr>
			<td>Extruded acrylic sheet</td>
			<td>Professional Plastics</td>
			<td>SACR 062EF</td>
			<td>Thickness 0.062"</td>
		</tr>
		<tr>
			<td>Razor blade</td>
			<td>McMaster-Carr</td>
			<td>3962A3</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Tweezers</td>
			<td>McMaster-Carr</td>
			<td>8384A47</td>
			<td>#5 tip</td>
		</tr>
		<tr>
			<td>Super Glue Gel</td>
			<td>Loctite</td>
			<td>130380</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Air Brush</td>
			<td>Snap-on Industrial</td>
			<td>BF175TA</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Air Compressor</td>
			<td>Paasche</td>
			<td>B002YKN8YO</td>
			<td>D500</td>
		</tr>
		<tr>
			<td>Thermocouple</td>
			<td>Omega</td>
			<td>HH12A</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Hot plate</td>
			<td>Cimarec</td>
			<td>SP131325Q</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>CO2 direct-write laser</td>
			<td>VersaLaser</td>
			<td>3.5</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Dessicator</td>
			<td>Fisher Scientific</td>
			<td>08-595</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Lamp</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>custom-built</td>
		</tr>
		<tr>
			<td>Microtensile tester</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>custom-built</td>
		</tr>
	</tbody>
</table>

environmentally-controlled microtensile testing of mechanically-adaptive polymer nanocomposites for ex vivo characterization

Implanterbare microdevices vinder stor opmærksomhed i flere biomedicinske anvendelser 1-4. Sådanne enheder er blevet lavet af en række materialer, der hver tilbyder sine egne fordele og mangler 5,6. Mest fremtrædende grund mikroskala enhed dimensioner, er en højt modul nødvendig for at lette implantation i levende væv. Omvendt bør stivheden af indretningen matcher det omgivende væv for at minimere induceret lokal stamme 7-9. Derfor har vi for nylig udviklet en ny klasse af bio-inspirerede materialer til at opfylde disse krav ved at reagere på miljømæssige stimuli med en ændring i mekaniske egenskaber 10-14. Specifikt vores poly (vinylacetat)-baserede nanocomposite (PVAc-NC) viser en reduktion i stivhed, når de udsættes for vand og forhøjede temperaturer (f.eks kropstemperatur). Desværre par metoder eksisterer for at kvantificere stivhed af materialer in vivo 15 og mechnisk afprøvning uden for fysiologisk miljø kræver ofte store prøver uhensigtsmæssigt for implantation. Endvidere kan stimuli-responderende materialer hurtigt at genvinde deres oprindelige stivhed efter explantation. Derfor har vi udviklet en fremgangsmåde, ved hvilken de mekaniske egenskaber af implanterede microsamples kan måles ex vivo, med simulerede fysiologiske betingelser opretholdes ved hjælp fugt og temperaturstyring 13,16,17. Til dette formål blev en brugerdefineret microtensile tester designet til at rumme mikroskala prøver 13,17 med bredt varierende Youngs moduli (mellem 10 MPa til 5 GPa). Da vores interesser er i anvendelsen af ​​PVAc-NC som en biologisk-tilpasningsdygtige neural sonde substrat, et værktøj i stand til mekanisk karakterisering af prøver på mikroskala var nødvendig. Dette værktøj blev tilpasset for at give fugtighed og temperatur kontrol, hvilket minimerede prøve tørring og køling 17. Som et resultat, mekaniker denal kendetegn eksplanterede prøve nøje afspejler dem af prøven lige før eksplantation. Det overordnede mål med denne metode er at kvantitativt vurdere in vivo mekaniske egenskaber, specifikt Youngs modul, af stimuli-lydhør, mekanisk adaptive polymer-baserede materialer. Dette opnås ved først om de miljømæssige forhold, der minimerer en ændring i prøve mekaniske egenskaber efter eksplantation uden at bidrage til en reduktion i stivhed uafhængig af resultatet af implantation. Prøver fremstilles derefter til implantation, håndtering og testning (figur 1A). Hver prøve implanteres i hjernebarken hos rotter, der er repræsenteret her som en eksplanteret rottehjerne, i et bestemt tidsrum (figur 1B). På dette punkt er prøven eksplanteret og straks fyldt i microtensile testeren, og derefter underkastet trækprøvning (figur1C). Efterfølgende data analyse giver indsigt i den mekaniske opførsel af disse innovative materialer i miljøet af hjernebarken.

De mekaniske egenskaber af næsten alle polymere materialer, herunder vores PVAc-NC, afhænger udsættelse for miljømæssige betingelser. Mest bemærkelsesværdigt, disse omfatter udsættelse for varme og fugt. Når et materiale blødgøres grund af optagelse af fugt, eller undergår en termisk overgang, det viser en reduktion i Youngs modul. Ved udarbejdelsen af fugt-og temperatur-kontrolleret miljø for ex vivo prøve mekanisk karakterisering, er det vigtigt at sikre, at der er minimal ændring i vandindhold...

Watch this Scientific Journal Video about Environmentally-controlled Microtensile Testing of Mechanically-adaptive Polymer Nanocomposites for ex vivo Characterization at JoVE.com

Environmentally-controlled Microtensile Testing of Mechanically-adaptive Polymer Nanocomposites for ex vivo Characterization

En fremgangsmåde er beskrevet, hvorved<em&gt; In vivo</em&gt; Mekaniske opførsel af stimuli-responsive materialer overvåges som en funktion af tiden. Prøver testes<em&gt; Ex vivo</em&gt; Ved hjælp af en microtensile tester med miljøkontrol at simulere fysiologiske miljø. Dette arbejde yderligere fremmer forståelsen af<em&gt; In vivo</em&gt; Opførsel af vores materiale.

Miljømæssigt-kontrolleret Microtensile Afprøvning af Mekanisk adaptive Polymer Nanokompositter for<em&gt; Ex vivo</em&gt; Karakterisering

Implantable microdevices are gaining significant attention for several biomedical applications1-4. Such devices have been made from a range of materials, ...

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Environmentally-controlled Microtensile Testing of Mechanically-adaptive Polymer Nanocomposites for ex vivo Characterization

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