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Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

DOI :

10.3791/50081-v

December 6th, 2012

December 6th, 2012

13,912 Views

1Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis

The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.

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