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09:21 min
December 6th, 2012
DOI :
10.3791/50081-v
Chapters
0:05
Title
2:01
Material Preparation and Sterilization
3:26
Cell Preparation
4:46
Hydrogel Fabrication and Cell Encapsulation
5:57
Chymotrypsin Mediated Gel Erosion and Spheroid Recovery
7:24
Results: Spheroid Encapsulation, Survival, Proliferation, and Recovery in Thiol-ene Hydrogels
8:31
Conclusion
Summary
下面的协议提供了用于封装在逐步增长的PEG-肽水凝胶形成的硫醇 - 烯照片点击反应胰腺β-细胞的技术。这种材料平台不仅提供了细胞相容性的微环境,细胞封装,但也允许用户控制的快速恢复细胞结构内形成的水凝胶。
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