December 6th, 2012
•The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.
Tags
Related Videos
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
Robotic Mirror Therapy System for Functional Recovery of Hemiplegic Arms
Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved