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DOI :
10.3791/50083-v
•
8:59 min
April 5th, 2013
Chapters
0:05
Title
1:46
Yeast Preparations
2:51
Plate\Slide Preparations
3:34
Microscope Preparations
4:58
Imaging
6:18
Results: Modeling Protein Misfolding with GFP-Ubc9ts
8:23
Conclusion
细胞存活率依赖于蛋白质的错误折叠的及时和有效地管理。在这里,我们描述了一种方法,可视化的错误折叠的蛋白质复性,退化,或夹杂物封存在不同的潜在的命运。我们演示了如何使用的折叠式传感器,UBC9 TS
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