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DOI :
10.3791/50101-v
December 26th, 2012
Chapters
0:05
Title
0:58
Fixing Yeast Communities
3:40
Sectioning Frozen Communities for Fluorescence Imaging
5:15
Results: Fluorescence Images of Vertical Cross-sections of Yeast Communities
6:44
Conclusion
우리는 형광 세포의 내부 패턴을 관찰 할 효모 커뮤니티를 동결 cryosectioning을위한 프로토콜을 제시한다. 방법은 메탄올 - 고정에 의존하고 지역 사회 내에서 형광 단백질의 운영을 중지시키지 않고 세포의 공간 분포를 유지하기 위해 월 퍼가기.
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