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DOI :
10.3791/50113-v
•
13:34 min
September 29th, 2013
Chapters
0:05
Title
2:02
GBT-T1 Gene Trap Vector Design
6:37
Screening of the F0 Fish
10:00
Screening of the F1 Fish
11:28
Results: Gene Trapping with Gal4-VP16
13:02
Conclusion
3:15
Production of the F0 Generation by Microinjection of the Gene Trap
本协议描述的使用GAL4-VP16为主要记者和GFP / RFP在斑马鱼次要记者基因陷阱插入突变的方法。约于一体的高表达F0鱼产量的基因陷阱后代共同表达GFP和RFP十名。筛选过程可以容易地缩放,以适应执行插入诱变屏幕实验室的规模。
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