Name: multi-unit recording methods to characterize neural activity in the locust (schistocerca americana) olfactory circuits
Uploaded: 2013-01-25T12:00:00
Description: Watch this Scientific Journal Video about Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits at JoVE.com

Forfatterne vil gerne takke følgende for at finansiere dette værk: generøse opstart midler fra Institut for Biomedical Engineering i Washington University, en McDonnell Center for Systems Neuroscience tilskud, en Office of Naval Research tilskud (Grant #: N000141210089) til BR

1. Lugt Forberedelse og levering <ol><li> Fortynd lugt opløsninger i mineralolie i volumen for at opnå det ønskede koncentrationsniveau. Gemme en 20 ml blanding af mineralolie og lugtstoffet i en 60 ml glasflaske. Indsætte to injektionsnåle til en gummiprop (gauge 19), en fra bunden og den anden fra toppen for at tilvejebringe et indløb og et udløb linje. Tætne glasflaske med denne gummiprop og vedhæfte et designet aktivt kulfilter til indløbsledningen (figur 1A). </li><li> Kulfilter fremsti...

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L., Joseph, J., Stopfer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Encoding+a+temporally+structured+stimulus+with+a+temporally+structured+neural+representation.">Encoding a temporally structured stimulus with a temporally structured neural representation.</a> Nature Neuroscience. 8, 1568-1576 (2005).</li><li>MacLeod, K., Laurent, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinct+mechanism+for+synchronization+and+temporal+patterning+of+odor-encoding+neural+assemblies.">Distinct mechanism for synchronization and temporal patterning of odor-encoding neural assemblies.</a> Science. 274, 976-979 (1996).</li><li>Wehr, M., Laurent, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+afferent+and+central+temporal+patterns+in+the+locust+olfactory+system.">Relationship between afferent and central temporal patterns in the locust olfactory system.</a> The Journal of Neuroscience. 19, 381-390 (1999).</li><li>Moreaux, L., Laurent, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estimating+firing+rates+from+calcium+signals+in+locust+projection+neurons+in+vivo.">Estimating firing rates from calcium signals in locust projection neurons in vivo.</a> Frontiers in Neural Circuits. 1, 1-13 (2007).</li><li>Galizia, C. G., Joerges, J., Kuttner, A., Faber, T., Menzel, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+semi-in-vivo+preparation+for+optical+recording+of+the+insect+brain.">A semi-in-vivo preparation for optical recording of the insect brain.</a> Journal of Neuroscience Methods. 76, 61-69 (1997).</li><li>Galan, R. F., Sachse, S., Galizia, C. G., Hez, A. V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odor-driven+attractor+dynamics+in+the+antennal+lobe+allow+for+simple+and+rapid+olfactory+pattern+classification.">Odor-driven attractor dynamics in the antennal lobe allow for simple and rapid olfactory pattern classification.</a> Neural Computation. 16, 999-1012 (2004).</li><li>Kuebler, L. S., Schubert, M., Karpati, Z., Hansson, B. S., Olsson, S. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antennal+Lobe+Processing+Correlates+to+Moth+Olfactory+Behavior.">Antennal Lobe Processing Correlates to Moth Olfactory Behavior.</a> Journal of Neuroscience. 32, 5772-5782 (2012).</li><li>Silbering, A. F., Bell, R., Galizia, C. G., Benton, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calcium+Imaging+of+Odor-evoked+Responses+in+the+Drosophila+Antennal+Lobe.">Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe.</a> J. Vis. Exp. (61), e2976 (2012).</li><li>Skiri, H. T., Galizia, C. G., Mustaparta, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Representation+of+Primary+Plant+Odorants+in+the+Antennal+Lobe+of+the+Moth+Heliothis+virescens+Using+Calcium+Imaging.">Representation of Primary Plant Odorants in the Antennal Lobe of the Moth Heliothis virescens Using Calcium Imaging.</a> Chemical Senses. 29, 253-267 (2004).</li></ol>

Mest sensoriske stimuli fremkalder kombinatoriske reaktioner, der distribueres på tværs af ensembler af neuroner. Derfor samtidig overvågning af multi-neuron aktivitet er nødvendig for at forstå, hvordan stimulus-specifikke oplysninger er repræsenteret og bearbejdes af neurale kredsløb i hjernen. Her har vi vist ekstracellulære flere enheder optagelse teknikker til karakterisering lugt-fremkaldte reaktioner i de første tre behandlingscentre langs insekt olfaktoriske pathway. Vi bemærker, at de teknikker, der p...

<table border="1"><tbody><tr><td> Navn </td><td> Firma </td><td> Catalog Number </td><td> Kommentarer </td></tr><tr><td></td><td></td><td></td><td> Elektrofysiologi Udstyr </td></tr><tr><td> AC-forstærker </td><td> GRASS </td><td> Model P55 </td><td> for enlige sensillum optagelser </td></tr><tr><td> Audio monitor (model 3300) </td><td> AM Systems </td><td> 940.000 </td><td></td></tr><tr><td> Specialfremstillede 16-kanals forforstærker og forstærker </td><td> Cal. Tech. Biologi Electronics Shop </td><td></td><td> for AL og MB-optagelser </td></tr><tr><td> Dataindsamlingsenheden </td><td> National Instruments </td><td> BNC-2090 </td><td></td></tr><tr><td> Fiberoptisk lys </td><td> WPI </td><td> SI-72-8 </td><td> &lt;/ Td&gt; </tr><tr><td> Lyskilden 115 V </td><td> WPI </td><td> NOVA </td><td></td></tr><tr><td> Manuel mikromanipulator </td><td> WPI </td><td> M3301R </td><td> for johannesbrød hjernen optagelser </td></tr><tr><td> Stereomicroscope1 om boom stativ </td><td> Leica </td><td> M80 </td><td> for johannesbrød hjernen optagelser </td></tr><tr><td> Stereomicroscope2 </td><td> Leica </td><td> M205C </td><td> for enlige sensillum optagelser </td></tr><tr><td> Vibration-isolation bord </td><td> TMC </td><td> 63-500 serien </td><td></td></tr><tr><td> Motoriseret mikromanipulator </td><td> Sutter Instruments </td><td> MP285 / T </td><td></td></tr><tr><td> Oscilloscope </td><td> Tektronix </td><td> TD2014B </td><td></td></tr><tr><td></td><td></td><td></td><td> Elektroder / Byggeri Værktøjer &lt;/ Td&gt; </tr><tr><td> 16-kanals elektrode </td><td> NeuroNexus </td><td> A2x2-tet-3mm-150-121 </td><td> for antennal lap optagelser </td></tr><tr><td> Borsilikat kapillarrør med glødetråd, ID 0,69 mm </td><td> Sutter Instruments </td><td> BF120-69-10 </td><td> til fremstilling af glaselektroder </td></tr><tr><td> Mikropipette aftrækker </td><td> Sutter Instruments </td><td> P-1000 </td><td></td></tr><tr><td> Funktionsgenerator </td><td> Multimeter Warehouse </td><td> SG1639A </td><td> for overregulering elektroder </td></tr><tr><td> Guldbelægning opløsning (non cyanid) </td><td> SIFCO Industries </td><td> NC SPS 5355 </td><td></td></tr><tr><td> Impedans tester </td><td> BAK Electronics Inc. </td><td> IMP-2 </td><td> for overregulering elektroder </td></tr><tr><td> Skift roterende </td><td> Electroswitch </td><td> C7D0123N </td><td> for guld-plstilstande elektroder </td></tr><tr><td> Impulsisolator </td><td> WPI </td><td> A365 </td><td> for overregulering elektroder </td></tr><tr><td> Q-serien elektrodeholder </td><td> Warner Instruments </td><td> 64-1091 </td><td></td></tr><tr><td> Sølvtråd 0,010 &quot;diameter </td><td> AM Systems </td><td> 782.500 </td><td> jordforbindelseselektrode </td></tr><tr><td> 8 pin DIP IC-sokkel </td><td> Digikey </td><td> ED90032-ND </td><td></td></tr><tr><td> Borsilikat kapillarrør med glødetråd, ID 0,58 mm </td><td> Warner Instruments </td><td> 64-0787 </td><td> snoet wire tetrode konstruktion </td></tr><tr><td> Varmepistol </td><td> Weller </td><td> 6966C </td><td></td></tr><tr><td> Rediohm-800 wire </td><td> Kanthal Precision Technologies </td><td> PF002005 </td><td></td></tr><tr><td> Titer pladeryster </td><td> ThermoVidenskabelig </td><td> 4625Q </td><td> snoede ledninger </td></tr><tr><td> Carbide saks, 4,5 &quot; </td><td> Biomedical Research Instr </td><td> 25-1000 </td><td> til skæring af snoede tråde tetrode </td></tr><tr><td> Fin spids pincet </td><td> HECO </td><td> 91-EF5-SA </td><td> for drilleri tetrode ledninger fra hinanden </td></tr><tr><td></td><td></td><td></td><td> Lugt Levering </td></tr><tr><td> 6 ml sprøjte </td><td> Kendall </td><td> 1180600777 </td><td> for specialdesignet aktivt kulfilter </td></tr><tr><td> Brown lugt flasker </td><td> Fisher </td><td> 08-912-165 </td><td></td></tr><tr><td> Kul </td><td> <a href="http://BuyActivatedCharcoal.com" target="_blank">BuyActivatedCharcoal.com</a> </td><td> GAC-48C </td><td></td></tr><tr><td> Desiccant </td><td> Drierite </td><td> 23.005 </td><td></td></tr><tr><td> Drierite gastørring krukke </td><td> Fischer Scientific </td><td> 09-204 </td><td></td></tr><tr><td> Varmekrymperør </td><td> 3M </td><td> EPS-200 </td><td> lugtfilter forberedelse </td></tr><tr><td> Kanyle aluminium hub, gauge 19 </td><td> Kendall </td><td> 8881-200136 </td><td> til tilvejebringelse af indgangs-og udgangslinier for lugt-flasker </td></tr><tr><td> Mineralolie </td><td> Mallinckrodt Chemicals </td><td> 6357-04 </td><td> for lugt fortynding </td></tr><tr><td> Nalgene plastslange, 890 FEP </td><td> Thermo Scientific </td><td> 8050-0310 </td><td> for bæregas levering </td></tr><tr><td> Pneumatisk picopump </td><td> WPI </td><td> sys-pv820 </td><td> for lugt levering </td></tr><tr><td> Polyethylenrør ID 0,86 mm </td><td> Intramedic </td><td> 427.421 </td><td> for lugt flaske udløb connections og saltvand overflod slanger </td></tr><tr><td> Propper </td><td> Lab Pure </td><td> 97.041 </td><td> til tætning lugt flasker </td></tr><tr><td> Time tape </td><td> PDC </td><td> T-534-RP </td><td></td></tr><tr><td> Tubing luer </td><td> Cole-Parmer </td><td> 30.600-66 </td><td></td></tr><tr><td> Vakuum rør </td><td> McMaster-Carr </td><td> 5488K66 </td><td></td></tr><tr><td></td><td></td><td></td><td> Forberedelse / Dissection </td></tr><tr><td> 100 x 15 mm petriskål </td><td> VWR International </td><td> 89000-304 </td><td></td></tr><tr><td> 18 AWG kobber snoet tråd </td><td> Lapp Kabel </td><td> 4510013 </td><td> ledningsisolering anvendes som gummipakninger </td></tr><tr><td> 22 AWG snoet hookup ledning </td><td> Alphawire </td><td> 1551 </td><td> hjerneplatform </td></tr><tr><td> Batik voks </td><td> Jacquard </td><td> 7946000 </td><td></td></tr><tr><td> Dental periferi Wax </td><td> Henry-Schein Dental </td><td> 6652151 </td><td></td></tr><tr><td> Electrowaxer </td><td> Almore International </td><td> 66.000 </td><td></td></tr><tr><td> Epoxy, 5 min </td><td> Permatex </td><td> 84.101 </td><td></td></tr><tr><td> Injektionsnål aluminiumskapper </td><td> Kendall </td><td> 8881-200136 </td><td></td></tr><tr><td> Protease fra Streptomyces griseus </td><td> Sigma-Aldrich </td><td> P5147 </td><td> for desheathing locust hjerne </td></tr><tr><td> Suturtråd usterile </td><td> Fisher </td><td> NC9087024 </td><td> for at binde maven efter tarmen fjernelse </td></tr><tr><td> Vetbond </td><td> 3M </td><td> 1469SB </td><td> til tætning amputation sites </td></tr><tr><td> Dumont # 1 pincet (grov) </td><td> WPI </td><td> 500.335 </td><td></td></tr><tr><td> Dumont # 5 titanium tang (fin) </td><td> WPI </td><td> 14.096 </td><td></td></tr><tr><td> Dumont # 5SF pincet (super-fin) </td><td> WPI </td><td> 500.085 </td><td> desheathing locust hjerne </td></tr><tr><td> 10 cm dissekere saks </td><td> WPI </td><td> 14.393 </td><td> til fjernelse af ben og vinger </td></tr><tr><td> Vannas saks (fin) </td><td> WPI </td><td> 500.086 </td><td> til fjernelse kutikula, skære foregut </td></tr><tr><td></td><td></td><td></td><td> Saline Profusion </td></tr><tr><td> Extension sæt med rate flowregulatoren </td><td> Moore Medical </td><td> 69.136 </td><td> til regulering saltvand flow </td></tr><tr><td> IV indgivelsessættet medY injektionssted </td><td> Moore Medical </td><td> 73.190 </td><td> til regulering saltvand flow </td></tr></tbody></table>

multi-unit recording methods to characterize neural activity in the locust (schistocerca americana) olfactory circuits

Påvisning og fortolkning af olfaktoriske cues er afgørende for overlevelsen af ​​mange organismer. Bemærkelsesværdigt er arter på tværs phyla slående ens olfaktoriske systemer, tyder på, at den biologiske tilgang til kemisk sensing er blevet optimeret over evolutionær tid 1. I det insekt olfaktoriske system, er lugtstoffer transduceret af olfaktoriske receptor neuroner (ORN) i antennen, som konverterer kemiske stimuli i tog af aktionspotentialer. Sensorisk input fra ORNs derpå videresendes til antennal lap (AL, en struktur analog til hvirveldyret lugtekolben). I AL, tager neurale repræsentationer for lugte i form af Spatiotemporal fyring mønstre fordelt på ensembler af de vigtigste neuroner (PNS, også kaldet projektion neuroner) 2,3. AL-output forarbejdes derefter af Kenyon celler (KCS) i den nedstrøms svamp organ (MB), en struktur forbundet med olfaktoriske hukommelse og indlæring 4,5. Hendese, præsenterer vi elektrofysiologiske optagelse teknikker til overvågning lugt-evoked neurale reaktioner i disse olfaktoriske kredsløb. Først præsenterer vi en enkelt sensillum optagelse metode til at studere lugt-evoked reaktioner på niveau med bestande af ORNs 6,7. Vi diskuterer brugen af ​​saltvand fyldt skærpet glaspipetter som elektroder til ekstracellulært overvåge Örn svar. Dernæst præsenterer vi en metode til ekstracellulært overvåge PN svar ved hjælp af en kommerciel 16-kanals elektrode 3. En lignende tilgang med en skræddersyet 8-kanals snoet wire tetrode påvises for Kenyon celle optagelser 8. Vi leverer oplysninger om vores eksperimentel opstilling og nuværende repræsentative optagelse spor for hver af disse teknikker.

Lugt-fremkaldte reaktioner af en enkelt ORN til to forskellige alkoholer er vist i figur 3D. Afhængigt af optagestedet (sensilla type, placering af elektroden) med flere enheder optagelser kan opnås. En rå ekstracellulære bølgeform fra en AL-optagelse er vist i figur 6A. Action potentialer eller pigge af varierende amplituder fra forskellige PNS kan observeres i denne spænding spor. Selvom locust antennal lap har excitatoriske projektion neuroner og h?...

Watch this Scientific Journal Video about Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits at JoVE.com

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

Vi viser variationer af den ekstracellulære flere enheder optagelsesteknik at karakterisere lugt-fremkaldte reaktioner i de tre første faser af hvirvelløse olfaktoriske pathway. Disse teknikker kan let tilpasses til at undersøge ensemble aktivitet i andre neurale systemer.

Multi-unit Optagelse metoder til karakterisering af neurale aktivitet i Locust (<em&gt; Schistocerca Americana</em&gt;) Lugtesansen Circuits

Detection and interpretation of olfactory cues are critical for the survival  of many organisms. Remarkably, species across phyla have strikingly similar ...

Research

JoVE Journal

Neuroscience

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Surgical Implantation of Chronic Neural Electrodes for Recording Single Unit Activity and Electrocorticographic Signals

Kirurgisk implantation af Kroniske Neurale elektroder til Optagelse Single Unit Aktivitet og Electrocorticographic signaler

The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery.  Here we provide useful information for ...

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Optisk registrering af suprathreshold neurale aktivitet med enkelt-celle-og single-spike opløsning

Signaling of information in the vertebrate central nervous system is often carried by populations of neurons rather than individual neurons. Also ...

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings GCMR in the Insect Antennal Lobe

Identifikation af olfaktoriske Flygtige stoffer ved hjælp af gaskromatografi-Multi-unit Recordings (GCMR) i Insect Antennal Lobe

All  organisms inhabit a world full of sensory stimuli that determine their  behavioral and physiological response to their environment. Olfaction is ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

Optogenetic forstyrrelse af neurale aktivitet med laserbelysning i Semi-intakt<em&gt; Drosophila</em&gt; Larver in Motion

Drosophila larval locomotion is a splendid model system in developmental and physiological neuroscience, by virtue of the genetic accessibility of the ...

Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals

Brug Single Sensillum Optagelse til Detect olfaktoriske Neuron Responses af seng Bugs til Semiochemicals

The insect olfactory system plays an important role in detecting semiochemicals in the environment. In particular, the antennal sensilla which house ...

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

Ekstracellulær Registrering af neuronal aktivitet Kombineret med Microiontophoretic Anvendelse af Neuroaktive stoffer i Awake mus

Differences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and ...

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Optagelse Temperatur-induceret neuronal aktivitet gennem Overvågning Calcium Ændringer i det olfaktoriske pære af<em&gt; Xenopus laevis</em

The olfactory system, specialized in the detection, integration and processing of chemical molecules is likely the most thoroughly studied sensory system. ...

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Quadruple Immunostaining af Olfactory Bulb til Visualisering af Olfactory Sensory Axon Molecular Identity Codes

The mouse olfactory system is often used to study mechanisms of neural circuit formation&#12288;because of its simple anatomical structure. An Olfactory ...

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

Spids<em&gt; In vivo</em&gt; Elektrofysiologiske optagelser af lokale feltpotentialer og multi-enhed aktivitet fra Hyperdirect-banen i bedøvede rotter

Converging evidence shows that many neuropsychiatric diseases should be understood as disorders of large-scale neuronal networks. To better understand the ...