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61.1K Views
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07:50 min
September 6th, 2013
DOI :
10.3791/50154-v
Chapters
0:05
Title
5:29
Day 3: Culture of Neonatal Cardiomyocytes
6:01
Results: Culture of Neonatal Mouse Cardiomyocytes and Representative Immunofluorescent Images
7:03
Conclusion
1:33
Day 1: Isolation of the Cardiac Tissue from Neonatal Mice
2:53
Day 2: Enzymatic Tissue Digestion and Plating of Cells
Summary
原代小鼠心肌细胞培养物的肌原纤维组织和功能的研究的关键工具之一。以下协议描述了从新生小鼠心脏原发性心肌细胞的分离和培养。所得到的心肌细胞培养物随后可用于各种生物力学,生物化学和细胞生物学测定。
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