Name: measurement of tactile allodynia in a murine model of bacterial prostatitis
Uploaded: 2013-01-16T13:00:00
Description: Watch this Scientific Journal Video about Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis at JoVE.com

この研究は、NIH / NIDDKからの助成金1K01DK079019A2（PT）によってサポートされていました。

1。マウス接種のための細菌の準備以下は無菌条件下で行わなければなりません。 <ol><li>キャップで1 17×100mmのポリプロピレンチューブを取り、新鮮なルリア培地（LB）培地3 mlを加える。オートクレーブしたチップを使用して、ひずみCP1 2の一部凍結細菌のグリセロールストックを取り、LB培地を含むチューブに培養液の少量を転送します。酸素がまだ管を入力す...

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UPECによるマウス前立腺の感染における生体内細菌性前立腺炎の病態に関与している可能性のある事象のモデリング、CP / CPPSまたは慢性炎症における素因イベントなどが可能になります。方法は女性の尿路感染症7,14でUPECモデルに文学の大きな体によって細菌準備や点滴の引き分けに記載。モデルは、潜在的なワクチン候補と免疫調節のメカニズムをテストする、病因を研究す?...

<table border="1"><tbody><tr><td> 試薬の名称 </td><td> 会社 </td><td> カタログ番号 </td></tr><tr><td>培養管</td><td> BDファルコン</td><td> 352059 </td></tr><tr><td> LBブロスミラー</td><td> EMD </td><td> EM1.10285.0500 </td></tr><tr><td>ナルゲン遠心管</td><td>サーモ</td><td> 3118-0050 </td></tr><tr><td>イソフルレン</td><td>バトラーシャイン</td><td> NDC 11695-6776-1 </td></tr><tr><td>カテーテルニードル30G </td><td>ハミルトン</td><td> 91030 </td></tr><tr><td> PE10チューブ</td><td> BD intramedic </td><td> 427400 </td></tr><tr><td> von Freyフィラメント</td><td> Stoelting </td><td> 58025から31 </td></tr></tbody></table>

measurement of tactile allodynia in a murine model of bacterial prostatitis

尿路病原性大腸菌 （UPEC）は、尿路感染症や細菌性前立腺炎1に重要な役割を果たしている病原体である。我々は最近、UPECは、慢性骨盤痛2、慢性前立腺炎/慢性骨盤痛症候群（CP / CPPS）3,4の機能の開始に重要な役割を持っていることが示されている。臨床的に関連UPECによる前立腺の感染は、組織の損傷および自己免疫のメカニズム5の開始を含むことができるメカニズムを介して慢性痛を開始し、確立することができます。 前立腺内UPECの病因を理解するための課題は、操作に前立腺の相対的な到達不能です。我々は、それによって前立腺の上行性感染を確立雄マウスにUPECの臨床株を提供するために前述の尿道感染症法6を利用した 。ここでは、BAを標準化するための私達のプロトコルを記述cterial接種7と同様に細菌の点滴のために麻酔雄マウスをcatheterizingするための手順。 CP / CPPSは主に触覚アロディニア4の存在によって特徴付けられる。動作テストは呼ば内臓痛8-10起因する皮膚の痛覚過敏の概念に基づいていた。内臓組織における過敏焦点は通常非痛み刺激（アロディニア）に誇張された応答を可能にする皮膚の疼痛閾値を低減します。基盤となる内臓痛の強度に伴って増加する傾向がある異常反応で皮膚の結果に垂直力の応用。我々はUPEC細菌を持つ単一の感染に応答して時間をかけて接触性アロディニアを定量化するためのvon Frey繊維を利用したNOD / ShiLtJマウスでの方法論を記述する。

<html>我々は、細菌感染時に慢性的な痛みの有無をNOD / ShiLtjおよびC57BL/6J雄マウスを調べた。雄性マウス（5〜7週齢）尿道（ 図1）に生理食塩水や細菌を注入した。生理食塩水をたらしC57BL/6JマウスとUPEC感染C57BL/6Jマウスのvon Freyフィラメントと骨盤領域の機械的刺激は、実験の28日間コース（ 図1）の間に変更されなかった応答周波数が得られた。これとは対照的に、UPEC...

Watch this Scientific Journal Video about Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis at JoVE.com

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

前立腺の感染は、慢性前立腺炎における骨盤痛を媒介する要因かもしれません。我々は時間をかけて、マウスにおける接触性アロディニアを測定するための雄マウスと方法論の尿道への細菌の点滴、標準化された細菌の接種物の調製のための手順を記述している。

細菌性前立腺炎のマウスモデルにおける接触性アロディニアの測定

Uropathogenic Escherichia coli (UPEC) are pathogens that play an important role in urinary tract infections and bacterial prostatitis1. We have recently ...

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