Name: molecular beam mass spectrometry with tunable vacuum ultraviolet (vuv) synchrotron radiation
Uploaded: 2012-10-30T14:00:00
Description: Watch this Scientific Journal Video about Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation at JoVE.com

劳伦斯伯克利国家实验室的先进光源，进行了实验在化学动力学光束线和科学办公室基础能源科学办公室，美国能源部根据合同号DE-AC02-05CH11231支持，通过化学科学部。

1。样品装载<ol><li>删除后法兰，拆卸3/8“的不锈钢喷嘴管从设备（参见图1和图2），并确保它是干净和100毫米的孔是明确的（这可以通过寻找光源，通过它）。〜1毫升乙醇清洗，填充管，用棉花提示擦洗内。另外，在超声波浴中充满了肥皂和水或乙醇约20分钟，然后将喷嘴干燥的压缩空气。 </li><li>使用一个小的，干净的抹刀，地点约250mg样品在所述喷嘴的前部，靠近该?...

<ol>
	<li>Golan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ionization+of+dimethyluracil+dimers+leads+to+facile+proton+transfer+in+the+absence+of+hydrogen+bonds.">Ionization of dimethyluracil dimers leads to facile proton transfer in the absence of hydrogen bonds.</a> Nat. 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在超音速喷流产生的分子束的膨胀所产生的单体和二聚体。的DNA碱基的少量样品被放置在一个热蒸发源和加热以产生足够的蒸气压。氩气进行蒸气通过100微米口，通过2毫米的分离器，产生冷分子束14。可替换地，一个热情洋溢束源可以使用，其中样本被放置在与质谱仪的推斥极板（离子光学元件）连接到加热的烘箱。 我们使用真空紫外灯（7.4-25 eV）的，轻轻电离分子...

<table border="1"&gt;<tr&gt;<td<strong&gt;试剂/材料的名称</strong</td&gt;<td<strong&gt;公司</strong</td&gt;<td<strong&gt;产品目录号</strong</td&gt;<td<strong&gt;评论</strong</td&gt;</tr&gt;<tr&gt;<td&gt;尿嘧啶</td&gt;<td&gt; SIGMA</td&gt;<td&gt; U0750</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt; 1,3 - 二甲基脲嘧啶</td&gt;<td&gt;爱秩序</td&gt;<td&gt; 349801</td&gt;<td</td&gt;</tr&gt;</table

molecular beam mass spectrometry with tunable vacuum ultraviolet (vuv) synchrotron radiation

可调的软电离质谱耦合是一个功能强大的方法来研究孤立的分子复合物和集群及其光谱学和动力学1-4。生物分子的光电离过程的基础研究提供这些系统的电子结构有关的信息。此外，电离能和其他的生物分子在气相中的测定是不平凡的，这些实验提供了一个平台，产生这些数据。我们已经开发出一种汽化热技术加上超音速分子束，它提供一种温和的方式输送到气相中的这些物种。明智地的源气体和温度的组合允许用于形成二聚体和较高的DNA碱基簇。这个特定的工作的重点是， 即非共价相互作用，氢键，堆叠，和静电相互作用的影响，上的电离能和个体的生物分子，它们的配合，在微水1，5-9水化的质子转移。 我们已经进行了理论和实验的可视化特性的光电离动力学的气相尿嘧啶和1,3 -二甲基脲嘧啶二聚体，再加上分子束同步辐射化学动态学光束线的先进光源位于10和实验细节这里。这让我们观察1,3 -二甲基脲嘧啶二聚体内的质子传递，系统与π堆叠的几何形状和没有氢键1。分子束提供了一个非常方便和有效的方式来隔离环境扰动的回报可以准确的比较与电子结构计算，11，12样品的兴趣。通过调节光子的能量同步辐射光电离效率（PIE）曲线可以绘制告诉我们的阳离子电子态。然后这些值可以进行比较的理论模型和计算方法，反过来，详细解释了调查的种类1，3，电子结构和动力学。

图7示出了典型的质谱超音速膨胀的（A）的1,3 -二甲基尿嘧啶蒸气和PIE曲线的三个主要功能（在m / z 140的单体，在M / Z = 141的质子化的单体，和1的在M / Z 280）-1,3 - 二甲基脲嘧啶二聚体之间的真空紫外扫描摘录自8 eV和10 EV（B）。连续三次扫描，灰色的阴影是标准差。 <img alt="图1" fo:content-width="5in" fo:src="/files/ftp_upload/50164/50164fig1highres.jpg"...

Watch this Scientific Journal Video about Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation at JoVE.com

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation

加上可调谐的真空紫外同步辐射光电离质谱计在分子束提供了一个方便的工具来探索孤立的气相分子和簇的电子结构。 DNA碱基二聚体的质子转移机制，用这种方法鉴定。

可调式真空紫外（VUV）同步辐射分子束质谱分析

Tunable soft ionization coupled to mass spectroscopy is a powerful method to investigate isolated molecules, complexes and clusters and their spectroscopy ...

Research

JoVE Journal

Engineering

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Characterization of Electrode Materials for Lithium Ion and Sodium Ion Batteries Using Synchrotron Radiation Techniques

正极材料的同步辐射技术锂离子和钠离子电池表征

Intercalation compounds such as transition metal oxides or phosphates are the most commonly used electrode materials in Li-ion and Na-ion batteries. ...

科研

工程学

Fabrication of Gate-tunable Graphene Devices for Scanning Tunneling Microscopy Studies with Coulomb Impurities

对于扫描隧道显微学与库仑杂质制造门可调谐石墨烯器件

Owing to its relativistic low-energy charge carriers, the interaction between graphene and various impurities leads to a wealth of new physics and degrees ...

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

电池的使用同步为基础的硬X射线微断层故障分析

Imaging morphological changes that occur during the lifetime of rechargeable batteries is necessary to understand how these devices fail. Since the advent ...

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films

环境钚的薄膜弥散形态和生物利用度测量

The biological uptake of plutonium (Pu) in aquatic ecosystems is of particular concern since it is an alpha-particle emitter with long half-life which can ...

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

利用同步辐射微断层调查的多尺度三维微电子封装

Synchrotron radiation micro-tomography (SR&#181;T) is a non-destructive three-dimensional (3D) imaging technique that offers high flux for fast data ...

Plasma-assisted Molecular Beam Epitaxy of N-polar InAlN-barrier High-electron-mobility Transistors

N极性InAlN阻高电子迁移率晶体管的等离子体辅助分子束外延

Plasma-assisted molecular beam epitaxy is well suited for the epitaxial growth of III-nitride thin films and heterostructures with smooth, abrupt ...

Focused Ion Beam Fabrication of LiPON-based Solid-state Lithium-ion Nanobatteries for In Situ Testing

Focused Ion Beam Fabrication of LiPON-based Solid-state Lithium-ion Nanobatteries for In Situ Testing

基于 LiPON 的固态锂离子 Nanobatteries 的聚焦离子束制备方法在原位测试中的研究

Solid-state electrolytes are a promising replacement for current organic liquid electrolytes, enabling higher energy densities and improved safety of ...

Production and Characterization of Vacuum Deposited Organic Light Emitting Diodes

真空沉积有机发光二极管的生产与表征

A method for producing simple and efficient thermally-activated delayed fluorescence organic light-emitting diodes (OLEDs) based on guest-host or exciplex ...

Fabrication of Schottky Diodes on Zn-polar BeMgZnO/ZnO Heterostructure Grown by Plasma-assisted Molecular Beam Epitaxy

等离子体辅助分子束外延法制备的锌极性 BeMgZnO/氧化锌异质结构肖特基二极管的制备

Heterostructure field effect transistors (HFETs) utilizing a two dimensional electron gas (2DEG) channel have a great potential for high speed device ...

Quantitative Analysis by Thermogravimetry-Mass Spectrum Analysis for Reactions with Evolved Gases

热重分析-质谱分析对演化气体反应的定量分析

During energy conversion, material production, and metallurgy processes, reactions often have the features of unsteadiness, multistep, and ...