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Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

34.9K Views

09:51 min

March 5th, 2013

March 5th, 2013

34,899 Views

0:05

Title

1:22

Generation of Cell Lines Stably Expressing TetR

4:51

pTER and pT-Rex DEST30: shRNA or cDNAs Expression

5:30

Stable Cell Lines with Tetracycline-inducible Expression

8:31

Results: Stable Human Tet-on Cell Lines

9:27

Conclusion

A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.

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