Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA ExpressionMarta Gomez-Martinez 1, Debora Schmitz 2, Alexander Hergovich 1
1UCL Cancer Institute, 2Friedrich Miescher Institute for Biomedical Research
A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.
