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DOI :
10.3791/50195-v
August 8th, 2013
Chapters
0:05
Title
1:44
Metabolic Labeling with 4-thiouridine
3:05
Thiol-specific Biotinylation of Newly Transcribed RNA
7:00
Recovery of Newly Transcribed RNA
8:17
Results: Metabolic Labeling of Newly Transcribed RNA
10:25
Conclusion
5:09
Separation of Labeled and Unlabeled RNA Using Streptavidin-coated Magnetic Beads
细胞总RNA提供了一种用于研究的短期变化,以及在RNA合成和衰变动力学的RNA加工模板差。这里,我们描述遵循与4 - thiouridine的新转录的RNA的代谢标记通过硫醇特异性的生物素化和纯化的新转录的RNA,来克服这些限制。
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